Isolation and Identification of Pathogens of Maize Fusarium Stalk Rot in Ningxia

doi:10.11733/j.issn.1007-0435.2021.04.022

Acta Agrestia Sinica ›› 2021, Vol. 29 ›› Issue (4): 805-812.DOI: 10.11733/j.issn.1007-0435.2021.04.022

Previous Articles Next Articles

Isolation and Identification of Pathogens of Maize Fusarium Stalk Rot in Ningxia

ZHANG Xiao-jie¹, WANG Chun-ming², ZHOU Tian-wang², LIU Hao³, SHEN Rui-qing³, LI Min-quan^1*, GUO Cheng^1,2*

1. College of Plant Protection, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China;
2. Institute of Plant Protection, Gansu Academy of Agricultural Sciences, Lanzhou, Gansu Province 730070, China;
3 Plant Protection Institute, Ningxia Agriculture and Forestry Academy, Yinchuan, Ningxia 750002, China

Received:2020-12-01 Revised:2020-12-23 Published:2021-06-02
Contact: * E-mail:lmq@gsau.edu.cn; E-mail:gsguoch@126.com

宁夏地区玉米镰孢茎腐病病原菌的分离与鉴定

张小杰¹, 王春明², 周天旺², 刘浩³, 沈瑞清³, 李敏权^1*, 郭成^1,2*

1.甘肃农业大学植物保护学院, 甘肃兰州 730070;
2.甘肃省农业科学院植物保护研究所, 甘肃兰州 730070;
3.宁夏农林科学院植物保护研究所, 宁夏银川 750002

通讯作者: * E-mail:lmq@gsau.edu.cn; E-mail:gsguoch@126.com
作者简介:张小杰(1996-),女,甘肃文县人,硕士研究生,主要从事植物病理学研究,E-mail:zhangxj6262@126.com
基金资助:
宁夏农林科学院对外科技合作专项(DW-X-2018019);宁夏回族自治区重点研发项目(2018BBF02018-15)资助

Abstract

Abstract: In order to clarify the species diversity and dominant species of Fusarium causing maize stalk rot in Ningxia,in 2020,36 samples of maize stalk rot in Ningxia were collected. After tissue isolation,purification,and single-spore isolation,the obtained isolates were morphologically identified following Nelson and other classification systems,and molecularly determined according to their EF-1α sequences. The results showed that a total of 209 strains of Fusarium were obtained,including Fusarium graminearum species complex, F. verticillioides, F. equiseti, F. proliferatum, F. subglutinans, F. incarnatum,F. acuminatum,F. culmorum and F.avenaceum,whose isolation frequencies were 28.2%,23.9%,20.1%,8.1%,7.2%,6.7%,2.9%,1.9% and 1.0% respectively. Among them, F. graminearum species complex was distributed in all the five cities of Ningxia,and its pathogenicity was strong,so it was the dominant pathogen of maize stalk rot in Ningxia.

Key words: Ningxia, Maize Stalk Rot, Fusarium, TEF sequence identification

摘要： 为明确宁夏地区玉米镰孢茎腐病病原菌的种类及优势病原菌,本研究于2020年采集宁夏地区玉米(Zea mays L.)茎腐病样品36份,对所采集样品进行组织学分离、纯化和单孢分离,参照Nelson等分类系统进行形态学鉴定,并结合镰孢菌特异性引物EF-1α进行序列分析。本研究共获得209株镰孢菌,包括禾谷镰孢复合种(Fusarium graminearum species complex)、拟轮枝镰孢(F. verticillioides)、木贼镰孢(F. equiseti)、层出镰孢(F. proliferatum)、胶孢镰孢(F. subglutinans)、半裸镰孢(F. incarnatum)、锐顶镰孢(F.acuminatum.)、黄色镰孢(F.culmorum)和燕麦镰孢(F.avenaceum),其分离频率依次为28.2%,23.9%,20.1%,8.1%,7.2%,6.7%,2.9%,1.9%和1.0%。其中,禾谷镰孢复合种在宁夏5市均有分布,且致病性强,为宁夏地区玉米镰孢茎腐病优势致病菌。

关键词: 宁夏, 玉米茎腐病, 镰孢菌, TEF序列鉴定

CLC Number:

S435.131

ZHANG Xiao-jie, WANG Chun-ming, ZHOU Tian-wang, LIU Hao, SHEN Rui-qing, LI Min-quan, GUO Cheng. Isolation and Identification of Pathogens of Maize Fusarium Stalk Rot in Ningxia[J]. Acta Agrestia Sinica, 2021, 29(4): 805-812.

张小杰, 王春明, 周天旺, 刘浩, 沈瑞清, 李敏权, 郭成. 宁夏地区玉米镰孢茎腐病病原菌的分离与鉴定[J]. 草地学报, 2021, 29(4): 805-812.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2021.04.022

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2021/V29/I4/805

References

[1] 王春明,郭成,周天旺,等. 629份国内外玉米种质及杂交种对丝黑穗病的抗性评价[J]. 草地学报,2019,27(4):1075-1082
[2] 张治华. 宁夏粮食生产力布局结构演变及调整对策研究[J]. 粮食问题研究,2018,217(1):17-24
[3] 佘奎军,刘永进,贺奇,等. 2001-2015年宁夏玉米病虫害发生特征与损失分析[J]. 环境昆虫学报,2017,39(5):1071-1080
[4] 张超冲. 玉米镰刀菌茎腐病发生规律及防治试验[J]. 植物保护学报,1990,17(3):257-261
[5] 李新,许志斌,佘奎军,等. 宁夏玉米产业的现状和发展[J]. 种子,2009,28(9):104-106
[6] 石明亮,黄小兰,陆虎华,等. 玉米抗茎腐病研究进展及其鉴定与育种方法探讨[J]. 江苏农业科学,2017,45(4):1-4
[7] 马传禹,姚丽姗,杜腓利,等. 玉米抗茎腐病研究进展[J]. 玉米科学,2018,26(2):131-137
[8] Toman J,White D G. Inheritance of resistance to anthrancnose stalk rot of corn [J]. Phytopathology,1993,83(9):981-986
[9] 宋嘉琦. 辽宁省部分玉米品种茎腐病抗性及防治研究[D]. 沈阳:沈阳农业大学,2016:9
[10] 刘春来. 中国玉米茎腐病研究进展[J]. 中国农学通报,2017,33(30):130-134
[11] 陈楠,潘晓静,姚远,等. 东北地区玉米茎腐病镰孢菌EF-1α基因序列分析鉴定[J]. 玉米科学,2015,23(4):143-148
[12] 郭成. 甘肃玉米镰孢菌茎腐病病原菌多样性及抗性基因挖掘[D].兰州:甘肃农业大学,2019:26-27
[13] 刘树森,马红霞,郭宁,等. 黄淮海夏玉米主产区茎腐病主要病原菌及优势种分析[J]. 中国农业科学,2019,52(2):262-272
[14] 贺娟,何鹏飞,赵正龙,等. 云南省玉米茎基腐病病原镰孢菌的种群结构研究[J]. 玉米科学,2017,25(4):135-143
[15] 张超冲,李锦茂. 广西玉米青枯病调查和研究初报[J]. 广西农学院学报,1983(1):53-62
[16] 高卫东. 玉米茎腐病研究初报[J]. 山西农业大学学报,1983,3(2):88-91
[17] 单柳颖. 引起玉米茎腐病的镰孢菌的分离鉴定与多样性分析[D]. 北京:中国农业科学院,2018:3-7
[18] 赵志慧. 中国禾本科作物上镰孢菌属真菌分类的研究[D]. 沈阳:沈阳农业大学,2008:17-18
[19] Burgess L W,Summerell B A,Bullock S,et al. Laboratory manual for Fusarium research [M]. 3rd edition. Sydney:University of Sydney,1994:13-14
[20] Nelson P E,Toussoun T A,Marasas W F O. Fusarium species:An Illustrated Manual for Identification [M]. London:Pennsylvania State University Press,1983:1-193
[21] Leslie J F,Summerell B A. The Fusarium Laboratory Mannual[M]. USA:Blackwell Publishing,2006:1-388
[22] 张亚朵,刘佳,黄文坤,等. 河北廊坊大豆枯萎病病原镰刀菌的分子鉴定[J]. 植物病理学报,2018,48(6):738-747
[23] 宋佐衡,陈捷,刘伟成,等. 关于玉米茎腐病接种方法的探讨[J]. 辽宁农业科学,1992(1):21-24
[24] O’Donnell K,Ward T J,Geiser D M,et al. Genealogical concordance between the mating type locus and seven other nuclear genes supports formal recognition of nine phylogenetically distinct species within the Fusarium graminearum clade [J]. Fungal Genetics and Biology,2004,41(6):600-623
[25] 马秉元,李亚玲,段双科. 陕西省关中地区玉米青枯病病原菌及其致病性的研究[J]. 植物病理学报,1985,15(3):150-152
[26] Burgess L W,Summerell B A. Mycogeography of Fusarium:survey of Fusarium species in subtropical and semiarid grassland soils from Queensland,Australia. Mycological Research,1992,96(9):780-784
[27] 马甜,王俊波,张治华,等. 宁夏中部干旱带天然草地气候生产潜力研究[J]. 草地学报,2013,21(2):236-242

Isolation and Identification of Pathogens of Maize Fusarium Stalk Rot in Ningxia

宁夏地区玉米镰孢茎腐病病原菌的分离与鉴定

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 12

Recommended Articles

Metrics

Comments

[1]	NIE Ming-he, SHEN Yan, LU Ying, WANG Ke-xin, ZHANG Xiao-ju. Ecostoichiometric Characteristics of Dominant Plant Leaves-soil Ecology in Different Communities of Desert Steppe in Yanchi County,Ningxia [J]. Acta Agrestia Sinica, 2021, 29(1): 131-140.
[2]	BAO Wei-bin, WANG You-qi, Liu Peng, XIA Zi-shu, BAI Yi-ru, YANG Fan, ZHONG Yan-xia. Characteristics of Soil Desiccation and Soil Water Distribution in Different Land Types in Mountainous Area of Southern Ningxia [J]. Acta Agrestia Sinica, 2020, 28(3): 775-783.
[3]	FU Bing-zhe, CHANG Wei, LI Ze-ya, GAO Xue-qin. Comparative experiment of forage sorghum varieties in the Ningxia irrigation area [J]. Acta Agrestia Sinica, 2019, 27(6): 1751-1758.
[4]	YU Hai-long, MA Kai-bo, KANG Yang-mei, DU Ya-xian, HUANG Ju-ying. Analysis of Grassland Climate-soil Potential Productivity in the Middle Arid Region of Ningxia [J]. Acta Agrestia Sinica, 2018, 26(3): 625-630.
[5]	AO Hai-we, XIE Ying-zhong, LI Yong-hong, MA Jian-le. Adaptability of APSIM Model in Simulating Lucerne-Wheat-Millet Crop Rotation System in Haiyuan Region of Ningxia [J]. Acta Agrestia Sinica, 2016, 24(1): 146-155.
[6]	AO Hai-wei, XIE Ying-zhong, LI Yong-hong, SONG Nai-ping, MA Jian-le, MA Ming-de. Adaptability of APSIM Lucerne Model in Semiarid Area of Ningxia [J]. Acta Agrestia Sinica, 2014, 22(3): 535-541.
[7]	ZHANG Xiang-ru, CHENG Man, ZHU Fei-hua, AN Shao-shan. Carbon Mineralization Characteristics of the Typical Plant Litters of Semiarid Grassland in the Mountain Area of Southern Ningxia [J]. , 2014, 22(2): 277-282.
[8]	MA Tian, WANG Jun-bo, ZHANG Zhi-hua, XU Xiu-mei. Potential Climatic Productivity of Natural Grassland in the Middle Arid Zone of Ningxia [J]. , 2013, 21(2): 236-242.
[9]	ZHU Ren-bin, CHENG Ji-min, LIU Yong-jin, LI Wei-jun, WEI Lin. Floristic Study of Spermatophyte in Yunwu Mountain Natural Reserves of China [J]. Acta Agrestia Sinica, 2012, 20(3): 439-443.
[10]	ZHOU Hong-you, YANG He-tong, TANG Wen-hua. Occurrence of Fusarium Root Rot in Astragalus adsurgens and Identification of the Pathogens [J]. , 2004, 12(4): 285-288,297.
[11]	LI Min-quan, YANG Bao-sheng, HOU Jun, ZHANG Zi-he. Toxicity Measurement of Carbendazim on Fusarium spp. in Alfalfa [J]. , 2002, 10(4): 270-273.
[12]	CHENG Hong, DU Feng, ZHAO Ke-xue, MU Xing-min. Alfalfa Production Status and Way of Improvement in the Hilly South of NingXia Autonomous Region [J]. , 2002, 10(3): 231-236.