Abstract: The potted seedlings of 3-year old Lycium ruthenicum from Minqin and Yongjing provenances were used as the experimental materials. Using wind tunnel simulation test,the seedlings were stressed by wind-sand flow under the wind speed of 9 m·s^-1 for 1 h per day and lasting 4 days. The morphological changes of leaves during the stress were recorded and the physiological indexes of leaves on the 4^th and 11^th days were measured to explore the physiological response mechanism of L. ruthenicum to continuous wind-sand flow stress. The results showed that the leaves of L. ruthenicum were damaged,appeared brown patches,and wilted after continuous wind-sand flow stress,and the membrane permeability increased on the 11^th day. Compared with the control,the activities of catalase (CAT),superoxide dismutase (SOD) and proline (Pro) content increased;the malonaldehyde (MDA) content decreased significantly on the 11^th day in Minqin leaves. The activities of CAT,peroxidase (POD) and MDA content increased,and the maximum photochemical efficiency of PSⅡ (F_v/F_m) decreased significantly on the 4^th day in Yongjing leaves. The other fluorescence parameters of the two were different. The results provided a theoretical basis for the cultivation and breeding of L. ruthenicum in sandy area.

Key words: Lycium ruthenicum, Wind-sand flow stress, Chlorophyll fluorescence, Antioxidase, Osmotic adjustment

摘要： 以民勤和永靖种源3龄黑果枸杞（Lycium ruthenicum）盆栽实生苗为试验材料，采用风洞模拟试验，在9 m·s^-1风速下每天1 h，持续4 d进行风沙流胁迫，记录胁迫过程中叶片形态变化，测定第4 d和第11 d叶片生理指标，以期探究民勤、永靖黑果枸杞对持续风沙流胁迫的生理响应机制。结果表明：持续风沙流胁迫后黑果枸杞叶片受损，出现褐色斑块，萎蔫，第11 d膜透性增大；与对照相比，胁迫后民勤叶片过氧化氢酶（Catalase，CAT）、超氧化物歧化酶（Superoxide dismutase，SOD）活性及脯氨酸（Proline，Pro）含量增加，丙二醛（Malonaldehyde，MDA）含量在第11 d显著下降；永靖叶片CAT、过氧化物酶（Peroxidase，POD）活性，MDA含量增加，PSⅡ最大光化学效率（F_v/F_m）在第4 d显著下降。二者其余荧光参数变化不一。研究结果可为风沙地区黑果枸杞种植选育提供一定的理论依据。

关键词: 黑果枸杞, 风沙流胁迫, 叶绿素荧光, 抗氧化酶, 渗透调节