Cloning and Function Identification of MsMYB58 in Alfalfa under Drought Stress

doi:10.11733/j.issn.1007-0435.2023.12.005

Acta Agrestia Sinica ›› 2023, Vol. 31 ›› Issue (12): 3608-3616.DOI: 10.11733/j.issn.1007-0435.2023.12.005

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Cloning and Function Identification of MsMYB58 in Alfalfa under Drought Stress

LIU Jia, WANG Shao-peng, SHI Kun, ZHOU Le, WANG Zan

College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China

Received:2023-08-16 Revised:2023-10-06 Online:2023-12-15 Published:2024-01-03

紫花苜蓿MsMYB58基因克隆及抗旱功能鉴定

刘佳, 王少鹏, 史昆, 周仂, 王赞

中国农业大学草业科学与技术学院, 北京 100193

通讯作者: 王赞,E-mail:zanwang@cau.edu.cn
作者简介:刘佳(1993-)，女，汉族，内蒙古赤峰人，博士研究生，主要从事牧草基因资源挖掘与利用的相关研究，E-mail:liujia199301@163.com
基金资助:
北京市自然科学基金(6212015)；国家林业和草原种质资源库(2005DKA21003)资助

Abstract

Abstract: Drought is one of the important factors that limit the growth of alfalfa. MYB transcription factor widely involves in the regulation of plant growth and development and abiotic stress. In this study,cloning and bioinformatics analysis of alfalfa MsMYB58 gene were performed to analyze the function of MsMYB58 in response to drought stress. The results showed that the length of MsMYB58 open reading frame was 1 002 bp,encoding a protein containing 333 amino acids,and the molecular weight was 37.80 kDa,which was a representative member of the R2R3-MYB family,localizing in the nucleus,cell wall,and membrane. The tissue-specific expression of MsMYB58 was mainly in stems and the least in roots. The expression levels of MsMYB58 showed different trends in abscisic acid (ABA),salt (NaCl) and natural drought treatment. Under drought stress,overexpressing MsMYB58 in tobacco showed a significant increase in catalase,peroxidase and superoxide dismutase activities and proline content,a significant decrease in malondialdehyde content,a significant increase in chlorophyll fluorescence parameters Phi2 and F_v/F_m,and a significant decrease in NPQt. In this study,the function of alfalfa MsMYB58 in response to drought stress was investigated and provided a theoretical basis for further excavation of drought-responsive functional genes in alfalfa.

Key words: Alfalfa, Drought resistance, MsMYB58, Gene cloning

摘要： 干旱是限制紫花苜蓿(Medicago sativa)生长的重要环境限制因子，MYB转录因子广泛参与调控植物的生长发育和非生物胁迫。本研究对紫花苜蓿MsMYB58基因进行克隆及生物信息学分析，初步解析紫花苜蓿MsMYB58在干旱胁迫应答过程中的功能。结果显示，MsMYB58开放阅读框全长1 002 bp，编码蛋白含有333个氨基酸，分子量为37.80 kDa，属于典型的R2R3-MYB家族成员，定位在细胞核、细胞壁和细胞膜中。MsMYB58的组织特异性表达主要在茎中，在根中的表达最少；在脱落酸、盐和自然干旱处理中，MsMYB58的表达水平呈现不同的变化趋势。干旱胁迫下，过表达MsMYB58的转基因烟草的过氧化氢酶、过氧化物酶、超氧化物歧化酶的活性和脯氨酸含量显著增加，而丙二醛的含量则显著下降，叶绿素荧光参数PSⅡ利用效率和PSⅡ最大光化学量子产量显著升高，非光化学猝灭显著下降。本研究初步探究了紫花苜蓿MsMYB58在干旱胁迫应答过程中的功能，为进一步挖掘紫花苜蓿干旱响应功能基因提供理论基础。

关键词: 紫花苜蓿, 抗旱性, MsMYB58, 基因克隆

CLC Number:

S541

LIU Jia, WANG Shao-peng, SHI Kun, ZHOU Le, WANG Zan. Cloning and Function Identification of MsMYB58 in Alfalfa under Drought Stress[J]. Acta Agrestia Sinica, 2023, 31(12): 3608-3616.

刘佳, 王少鹏, 史昆, 周仂, 王赞. 紫花苜蓿MsMYB58基因克隆及抗旱功能鉴定[J]. 草地学报, 2023, 31(12): 3608-3616.

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Cloning and Function Identification of MsMYB58 in Alfalfa under Drought Stress

紫花苜蓿MsMYB58基因克隆及抗旱功能鉴定

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