Phylogeography and Genetic Structure of Artemisia halodendron Based on Single Copy Nuclear Genes

doi:10.11733/j.issn.1007-0435.2024.10.003

Acta Agrestia Sinica ›› 2024, Vol. 32 ›› Issue (10): 3034-3042.DOI: 10.11733/j.issn.1007-0435.2024.10.003

Phylogeography and Genetic Structure of Artemisia halodendron Based on Single Copy Nuclear Genes

LIU Meng-yao, WANG Tie-juan, XIU Fang, TANG Si-ru

College of Life Science and Technology, Inner Mongolia Normal University, Key Laboratory of Biodiversity Conservation and Sustainable Utilization in Mongolian Plateau for College and University of Inner Mongolia Autonomous Region, Hohhot, Inner Mongolia 010022, China

Received:2024-01-26 Revised:2024-03-24 Published:2024-11-04

基于单拷贝核基因的差不嘎蒿谱系地理与遗传结构研究

刘梦瑶, 王铁娟, 秀芳, 唐思如

内蒙古师范大学生命科学与技术学院, 内蒙古自治区高等学校蒙古高原生物多样性保护与可持续利用重点实验室, 内蒙古呼和浩特 010022

通讯作者: 王铁娟,E-mail:wtj105@163.com
作者简介:刘梦瑶(1996-)，女，汉族，内蒙古鄂尔多斯人，硕士研究生，主要从事植物生态学研究，Email:1143343811@qq.com
基金资助:
内蒙古自治区自然科学基金项目(2019MS03041);内蒙古师范大学基本科研业务费专项资金(2022 JBTD010)资助

Abstract

Abstract: To reveal the population genetic structure and historical dynamics of Artemisia halodendron,an important sand-fixing plant in northeast China,phylogeographic analysis was conducted on 154 Artemisia halodendron individuals from eight populations in the Hulunbuir Sandy Land and Horqin Sandy Land using two co-dominant nuclear genes in this study. The results showed that 38 and 47 haplotypes were detected by the two nuclear genes c7847 and c9002,respectively. The haplotype diversity(H_d) was 0.9474 and 0.9158,respectively,and all populations were above 0.8,which indicated had a high genetic diversity of A. halodendron. Analysis of molecular variance (AMOVA) showed that 96.524% and 96.985% of genetic variation existed within the populations of A. halodendron based on c9002 and c7847,respectively,with limited differentiation between populations and between the two sandy lands. The population genetic differentiation coefficients N_st were all higher than G_st,but the difference was not significant (P>0.05),indicating no obvious phylogeographic structure in A. halodendron. The neutral test showed that Tajima’s D values of the two nuclear genes were not significantly different (P>0.05),while Fu’s Fs values had significant differences (P<0.05),and combined with the mismatch distribution analysis,A. halodendron experienced limited population expansion under the neutral mode. Specifically,the New Barag Left Banner (DQ) and Hailar (HLR) populations coming from the Hulunbuir Sandy Land in the northern part of the distribution area,were most pronounced.

Key words: Artemisia halodendron, Single-copy nuclear genes, Genetic structure, Phylogeography

摘要： 为揭示我国东北部重要固沙植物差不嘎蒿(Artemisia halodendron)的群体遗传结构与种群历史动态，本研究利用2个直系同源单拷贝核基因对来自呼伦贝尔沙地和科尔沁沙地的8个种群154个个体进行分析。结果表明，2个核基因c7847和c9002分别检测到38和47个单倍型，单倍型多样性(H_d)分别为0.9474和0.9158，各种群均在0.8以上，反映出差不嘎蒿有着高的遗传多样性。2个基因的分子变异分析(AMOVA) 显示，差不嘎蒿96.524%和96.985%的遗传变异来自种群内，种群间和2个沙地间分化很小；种群遗传分化系数N_st均大于G_st，但差异不显著，表明差不嘎蒿没有明显的谱系地理结构。中性检验显示，2个核基因的Tajima’s D值均不显著，而Fu’s Fs值显著(P<0.05)，结合歧点分布分析，该种在中性模式下经历有限的种群扩张，其中分布区北部的呼伦贝尔沙地的新巴尔虎左旗(DQ)和海拉尔(HLR)种群最为明显。

关键词: 差不嘎蒿, 单拷贝核基因, 遗传结构, 谱系地理

CLC Number:

Q948.2

LIU Meng-yao, WANG Tie-juan, XIU Fang, TANG Si-ru. Phylogeography and Genetic Structure of Artemisia halodendron Based on Single Copy Nuclear Genes[J]. Acta Agrestia Sinica, 2024, 32(10): 3034-3042.

刘梦瑶, 王铁娟, 秀芳, 唐思如. 基于单拷贝核基因的差不嘎蒿谱系地理与遗传结构研究[J]. 草地学报, 2024, 32(10): 3034-3042.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2024.10.003

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2024/V32/I10/3034

References

[1] AVISE J C,ARNOLD J,BALL R M,et al. Intraspecific phylogeography:the mitochondrial DNA bridge between population genetics and systematics [J]. Annual Review of Ecology and Systematics,1987,18(1):489-522
[2] AVISE J C. Phylogeography:the history and formation of species[M]. Cambridge:Harvard University Press,2000:3-9
[3] QIU Y X,FU C X,COMES H P. Plant molecular phylogeography in China and adjacent regions:Tracing the genetic imprints of Quaternary climate and environmental change in the world’s most diverse temperate flora [J]. Molecular Phylogenetics and Evolution,2011,59 (1):225-244
[4] MENG H H,GAO X Y,HUANG J F,et al. Plant phylogeography in arid Northwest China:Retrospectives and perspectives[J]. Journal of Systematics and Evolution,2015,53(1):33-46
[5] SHI X J,ZHANG M L. Phylogeographical structure inferred from cpDNA sequence variation of Zygophyllum xanthoxylon across north-west China[J]. Journal of Plant Research,2015,128(2):269-282
[6] YIN H X，WANG L R,SHI Y,et al. The East Asian Winter Monsoon acts as a major selective factor in the intraspecific differentiation of drought-tolerant Nitraria tangutorum in Northwest China [J]. Plants,2020,9(9):1100
[7] 唐思如,王铁娟,秀芳,等. 基于单拷贝核基因的褐沙蒿遗传结构和谱系地理学分析[J]. 植物研究,2022,42(6):956-963
[8] 林有润. 论世界蒿属植物区系[J]. 植物研究,1995,15(1):1-37
[9] 林有润. 论蒿属的演化系统兼论蒿属与邻近属的亲缘关系[J]. 植物研究,1982,2(2):1-60
[10] 刘梦瑶,李伟琼,王铁娟. 差不嘎蒿茎叶解剖特征及其与气候因子的关系[J]. 草地学报,2021,29(5):1118-1124
[11] 王铁娟,杨持,吕桂芬,等. 中国北部六种沙蒿的地理替代规律及其主导生态因子[J]. 生态学报,2005,25(5):1012-1018
[12] 李进. 差不嘎蒿的分布及其在天然植被演替中的地位[J]. 中国沙漠,1991,11(2):55-60
[13] 王铁娟,李伟琼,张淑艳,等. 差不嘎蒿自然种群的遗传多样性与遗传分化[J]. 林业科学,2010,46(12):171-175
[14] 王铁娟,韩国栋. 中国蒿属沙生地理替代种亲缘关系的RAPD分析[J]. 植物研究,2007,27(4):449-454
[15] HUANG W D,ZHAO X Y,ZHAO X,et al. Relationship between the genetic diversity of Artemisia halodendron and climatic factors[J]. Acta Oecologica,2014,55:97-103
[16] 黄文达,赵学勇,左小安,等. 不同生境梯度下差不嘎蒿(Artemisia halodendron)种群ITS基因的序列分析[J]. 中国沙漠,2013,33(2):554-559
[17] HUANG W D,ZHAO X Y,ZHAO X,et al. Genetic diversity in Artemisia halodendron (Asteraceae) based on chloroplast DNA psbA-trnH region from different hydrothermal conditions in Horqin sandy land,northern China[J]. Plant Systematics and Evolution,2013,299(1):107-113
[18] 张天琪,杨光,刘峰,等. 呼伦贝尔沙地2000-2020年土地利用变化及生态服务价值[J]. 水土保持通报,2021,41(4):331-338,349
[19] 陈银萍,李晓辉,罗永清,等. 科尔沁不同沙地类型植被动态特征及其与凋落物的关系研究[J]. 草地学报,2021,29(1):115-120
[20] MISENER S,KRAWETZ S A. Methods in molecular biology[M].Totowa:Humana Press,2000:71-91
[21] KUMAR S,STECHER G,TAMURA K. MEGA7:Molecular evolutionary genetics analysis version 7.0 for bigger datasets[J]. Molecular Biology and Evolution,2016,33(7):1870-1874
[22] LIBRADO P,ROZAS J. DnaSP v5:A software for comprehensive analysis of DNA polymorphism data[J]. Bioinformatics,2009,25(11):1451-1452
[23] EXCOFFIER L,LISCHER H. Arlequin suite ver 3.5:a new series of programs to perform population genetics analyses under Linux and Windows[J]. Molecular Ecology Resources,2010,10(3):564-567
[24] PONS O,PETIT R J. Measuring and testing genetic differentiation with ordered versus unordered alleles[J]. Genetics,1996,144(3):1237-1245
[25] LEIGH J W,BRYANT D. POPART:full-feature software for haplotype network construction[J]. Methods in Ecology and Evolution,2015,6(9):1110-1116
[26] GRANT W S,BOWEN B W. Shallow population histories in deep evolutionary lineages of marine fishes:Insights from sardines and anchovies and lessons for conservation [J]. Journal of Heredity,1998,89(5):415-426
[27] WRIGHT S. The interpretation of population structure by F-statistics with special regard to systems of mating[J].Evolution,1965,19(3):395-420
[28] LIU Z F,ZHAO X X,WANG C S,et al. Magnetostratigraphy of Tertiary sediments from the Hoh Xil Basin:implications for the Cenozoic tectonic history of the Tibetan Plateau [J]. Geophysical Journal International,2003,154(2):233-252
[29] WANG W M. On the origin and development of Artemisia (Asteraceae) in the geological past [J]. Botanical Journal of the Linnean Society,2004,145(3):331-336
[30] 杨湘奎,杜绍敏,张烽龙. 呼伦贝尔高原晚更新世以来的古气候演变[J]. 自然灾害学报,2006,15(2):157-159
[31] 裘善文. 试论科尔沁沙地的形成与演变[J]. 地理科学,1989,9(4):317-328
[32] LI Z H,CHEN J,ZHAO G F,et al. Response of a desert shrub to past geological and climatic change:A phylogeographic study of Reaumuria soongarica (Tamaricaceae) in western China[J]. Journal of Systematics and Evolution,2012,50 (4):351-361
[33] XU Z,ZHANG M L. The effect of past climatic oscillations on spatial genetic structure of Atraphaxis manshurica (Polygonoideae) in the Horqin sand lands,northern China[J]. Biochemical Systematics and Ecology,2015,60:88-94(责任编辑闵芝智)第32卷第10期 Vol.32 No. 10草地学报 ACTAAGRESTIASINICA 2024年 10月

Phylogeography and Genetic Structure of Artemisia halodendron Based on Single Copy Nuclear Genes

基于单拷贝核基因的差不嘎蒿谱系地理与遗传结构研究

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 8

Recommended Articles

Metrics

Comments

[1]	CHUN Feng, ZHANG Feng, WU Yong-sheng, ZHAO Meng-li. Effects of Different Grazing Intensities on Biodiversity in the Stipa breviflora Desert Steppe [J]. Acta Agrestia Sinica, 2024, 32(3): 848-858.
[2]	WANG Jun-wei, MING Sheng-ping, XU Min, LA Qiong. Diversity Pattern and Phylogenetic Structure of Plant Communities in Alpine Ecological Key Zone [J]. Acta Agrestia Sinica, 2023, 31(9): 2777-2786.
[3]	GOU Tian-tian, MA Yan-na, JI Yi-nan, ZANG Guo-zhang, ZHENG Yi-qi. Study on Fine-scale Clonal Diversity and Spatial Genetic Structure of Bermudagrass [J]. Acta Agrestia Sinica, 2023, 31(1): 81-88.
[4]	ZHANG Xu, SU Shi-ping, SHI Wei-ning, Alizet Didi Dom, MA Qiang. Genetic Diversity Analysis of Reaumuria soongorica Populations [J]. Acta Agrestia Sinica, 2022, 30(9): 2336-2344.
[5]	LIU Meng-yao, LI Wei-qiong, WANG Tie-juan. Leaf and Stem Anatomical Structures of Artemisia halodendron and Their Relationship to Climatic Factors [J]. Acta Agrestia Sinica, 2021, 29(5): 1118-1124.
[6]	LIU Ya-ling, GENG Ya-ping, XIE Xiao-dong, WANG Fang, ZHANG Peng-fei. Genetic Diversity and Genetic Structure Analysis of Astragalus Based on SSR Molecular Marker [J]. Acta Agrestia Sinica, 2019, 27(5): 1154-1162.
[7]	XU Zhen-peng, WAN Tao, CAI Ping, ZHANG Xiao-ming, YI Wei-dong, WAN Yi-chao. Study on the Relevance between Genetic Diversity of Gymnocarpos przewalskii Populations and Geographic Climatic Factors [J]. Acta Agrestia Sinica, 2018, 26(1): 70-76.
[8]	YU Jing, JING Zhao-bin, CHENG Ji-min. Genetic Diversity of Stipa bungeana Trin. Populations in Shaanxi Analyzed by ISSR Markers [J]. Acta Agrestia Sinica, 2012, 20(3): 512-517.