Effects of Monoculture and Mixed Sowing on Soil Fungal Community Characteristics in Degraded Grasslands

doi:10.11733/j.issn.1007-0435.2025.10.008

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (10): 3205-3214.DOI: 10.11733/j.issn.1007-0435.2025.10.008

Effects of Monoculture and Mixed Sowing on Soil Fungal Community Characteristics in Degraded Grasslands

GAO Han-sen¹, YIN Ya-li^1,2,3, ZHAO Wen¹, LIU Jing-jing¹, LI Shi-xiong^1,2,3

1. Qinghai University, Xining, Qinghai Province 810016, China;
2. Qinghai Provincial Key Laboratory of Adaptive Management on Alpine Grassland, Xining, Qinghai Province 810016, China;
3. Key Laboratory of Alpine Grassland Ecosystem in the Three-River-Source, Ministry of Education, Xining, Qinghai Province 810016, China

Received:2024-12-04 Revised:2025-03-22 Published:2025-10-17

单播和混播对退化草地土壤真菌群落特征的影响

高汉森¹, 尹亚丽^1,2,3, 赵文¹, 刘晶晶¹, 李世雄^1,2,3

1. 青海大学, 青海西宁 810016;
2. 青海省高寒草地适应性管理重点实验室, 青海西宁 810016;
3. 三江源区高寒草地生态教育部重点实验室, 青海西宁 810016

通讯作者: 李世雄,E-mail:shixionglee@hotmail.com
作者简介:李世雄,E-mail:shixionglee@hotmail.com
基金资助:
青海省自然基金（编号：2023-ZJ-925M）；国家自然基金（编号：32260357）资助

Abstract

Abstract: Monoculture and mixed sowing are common ways to rehabilitate degraded grasslands in alpine meadows， but the effects of both on fungal community characteristics have not been fully clarified， and this study aimed to clarify the response and driving factors of soil fungal communities to monoculture and mixed sowing. this study took the extremely degraded grassland as a control， and took monoculture and mixed sowing grasslands established in the extremely degraded grasslands for 5 years as the objects to study the changes in the soil fungal community characteristics and soil physicochemical properties. The results showed that compared with the extremely degraded grassland， both monoculture and mixed sowing significantly changed theα-diversity indexes （P<0.05）， and there was significant difference in the β-diversity between them and the extremely degraded grassland （P<0.05）. Compared to extremely degraded grassland， monoculture significantly reduced （P<0.05） the abundance of Ascomycota and increased （P<0.05） the abundance of Mortierellomycota. Mixed sowing showed higher ecological network complexity and stability. Mixed sowing was more effective for soil nutrient restoration， and total nitrogen was the most important factor influencing fungi community structure and functional type. In summary， some of theα-diversity indicators and ecological network stability were higher in the mixed sowing treatment， and the soil nutrient restoration effect was better. Hence， the mixed sowing method should be applied more in the future black soil beach-type extreme degraded grassland management.

Key words: Monoculture, Mix sowing, Extremely-degraded grasslands, Soil fungal communities, Soil physical and chemical properties

摘要： 单播和混播是高寒草甸退化草地修复的常见方式，但是两者对真菌群落特征的影响尚未完全明确。为阐明土壤真菌群落对单播和混播的响应及其环境驱动因素，本研究以黑土滩极度退化草地为对照，以在黑土滩极度退化草地上建植5年的单播草地和混播草地为研究对象，探究土壤真菌群落特征及土壤理化性质变化。结果表明：相比于极度退化草地，单播与混播都显著改变了部分α指标（P<0.05），β多样性中两者都和极度退化草地之间存在显著差异（P<0.05）；相比于极度退化草地，单播显著降低了子囊菌门（Ascomycota）丰度（P<0.05），增加了被孢霉菌门（Mortierellomycota）丰度（P<0.05）；相比于极度退化草地，混播表现出更高的生态网络复杂性和稳定性；混播对于土壤养分的恢复效果更好，全氮是影响真菌群落结构和功能型的最主要因素。综上所述，混播处理中部分α多样性指标和生态网络稳定性更高，且对土壤养分的恢复效果更好，在往后的黑土滩治理中应该更侧重混播方式。

关键词: 单播, 混播, 极度退化草地, 土壤真菌群落, 土壤理化性质

CLC Number:

S812.2

GAO Han-sen, YIN Ya-li, ZHAO Wen, LIU Jing-jing, LI Shi-xiong. Effects of Monoculture and Mixed Sowing on Soil Fungal Community Characteristics in Degraded Grasslands[J]. Acta Agrestia Sinica, 2025, 33(10): 3205-3214.

高汉森, 尹亚丽, 赵文, 刘晶晶, 李世雄. 单播和混播对退化草地土壤真菌群落特征的影响[J]. 草地学报, 2025, 33(10): 3205-3214.

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Effects of Monoculture and Mixed Sowing on Soil Fungal Community Characteristics in Degraded Grasslands

单播和混播对退化草地土壤真菌群落特征的影响

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