Comprehensive Evaluation of Salt Tolerance of Oat （Avena sativa L.） Germplasm during the Germination Stage

doi:10.11733/j.issn.1007-0435.2026.05.014

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (5): 1717-1736.DOI: 10.11733/j.issn.1007-0435.2026.05.014

Comprehensive Evaluation of Salt Tolerance of Oat （Avena sativa L.） Germplasm during the Germination Stage

CHEN Jia-min^1,2, XU Shi-rui^1,4, JIA Bei-bei², CUI Shuo-zhen², WEI Guang-zhi², ZHANG Wei^2,3,4, ZHANG Mei-jun^2,3,4, JIA Ju-qing^2,3,4, LIU Long-long^1,4

1. Center for Agricultural Genetic, Resources Research, Shanxi Agricultural University, Taiyuan, Shanxi Province 030031, China;
2. College of Agriculture, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China;
3. Houji Laboratory in Shanxi Province, Taiyuan, Shanxi Province 030031, China;
4. Key Laboratory of Crop Germplasm Innovation and Molecular Breeding (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Taiyuan, Shanxi Province 030031, China

Received:2025-06-03 Revised:2025-08-09 Published:2026-05-08

燕麦萌发期耐盐性综合评价

陈佳敏^1,2, 徐世锐^1,4, 贾蓓蓓², 崔硕祯², 魏广智², 张威^2,3,4, 张美俊^2,3,4, 贾举庆^2,3,4, 刘龙龙^1,4

1. 山西农业大学农业基因资源研究中心, 山西太原 030031;
2. 山西农业大学农学院, 山西太谷 030801;
3. 山西省后稷实验室, 山西太原 030031;
4. 农业农村部种质创新与分子育种重点实验室(部省共建), 山西太原 030031

通讯作者: 刘龙龙，E-mail:lllong781211@sina.com;贾举庆，E-mail:jiajuqing@126.com
作者简介:陈佳敏（1999-），女，汉族，山西吕梁人，硕士研究生，主要从事作物遗传育种研究，E-mail:19834542925@163.com；
基金资助:
国家重点研发计划（2023YFD1202703）；财政部和农业农村部国家现代农业产业技术体系建设专项（CARS-07-A）；国家自然科学基金（32572378）；山西省重点研发计划项目（2022ZDYF110）；山西省后稷实验室自主立项课题（202304010930003-11）资助

Abstract

Abstract: The purpose of this study was to establish an efficient method for identifying the salt tolerance of oats （Avena sativa L.） during the germination stage， and to screen our excellent parents for salt-tolerant breeding. Using the petri dish paper germination method， 332 oat germplasms were subjected to a salt stress treatment with 0.80% （136.89 mmol·L^-1） NaCl solution. Eight indicators such as germination potential were measured. The salt tolerance was comprehensively evaluated and classified by combining the weighted membership function method with cluster analysis. Salt stress significantly inhibited all eight measured indicators. Correlation analysis revealed that highly significant positive correlations among these indicators. The 332 germplasms were classified into five salt tolerance grades： 34 highly salt-tolerant， 83 salt-tolerant， 116 moderately salt-tolerant， 51 salt-sensitive， and 48 highly salt-sensitive accessions. Regression analysis identified the salt tolerance index of root length （STI-RL） and the salt tolerance index of bud fresh weight （STI-BFW） as key indicators for evaluating salt tolerance during germination. Based on two-year evaluations， six materials demonstrated stable salt tolerance and can be utilized as parents in salt-tolerant breeding programs. Comparative analysis indicated that a higher proportion of Avena sativa L. accessions exhibited stronger salt tolerance compared to Avena nuda L. accessions. The 34 highly salt-tolerant germplasms provided excellent resources for salt-tolerant breeding and gene mining.

Key words: Oats, Germination stage, Weighted membership function analysis, Salt tolerance index

摘要： 本研究旨在建立高效的燕麦（Avena sativa L.）萌发期耐盐性鉴定方法，并为耐盐育种筛选优良亲本。采用培养皿纸上发芽法，以0.80%（136.89 mmol·L-1）NaCl溶液对332份燕麦种质进行盐胁迫处理，测定发芽势等8项指标，结合加权隶属函数法与聚类分析综合评价其耐盐性并划分等级。盐胁迫对8项指标均产生抑制；相关性分析显示各指标间存在极显著正相关；332份种质被划分为5个等级，包括34份高耐盐、83份耐盐、116份中等耐盐、51份盐敏感及48份高盐敏感种质；通过回归分析确定根长耐盐指数、芽鲜重耐盐指数为萌发期耐盐性评价的关键指标；根据两年评价结果，6份材料的耐盐性稳定，可作为耐盐育种亲本；比较分析显示，耐盐性皮燕麦的比率高于裸燕麦。34份高耐盐种质为耐盐育种与基因挖掘提供了优异资源。

关键词: 燕麦, 萌发期, 加权隶属函数分析, 耐盐指数

CLC Number:

S512.6

CHEN Jia-min, XU Shi-rui, JIA Bei-bei, CUI Shuo-zhen, WEI Guang-zhi, ZHANG Wei, ZHANG Mei-jun, JIA Ju-qing, LIU Long-long. Comprehensive Evaluation of Salt Tolerance of Oat （Avena sativa L.） Germplasm during the Germination Stage[J]. Acta Agrestia Sinica, 2026, 34(5): 1717-1736.

陈佳敏, 徐世锐, 贾蓓蓓, 崔硕祯, 魏广智, 张威, 张美俊, 贾举庆, 刘龙龙. 燕麦萌发期耐盐性综合评价[J]. 草地学报, 2026, 34(5): 1717-1736.

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Comprehensive Evaluation of Salt Tolerance of Oat （Avena sativa L.） Germplasm during the Germination Stage

燕麦萌发期耐盐性综合评价

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