Effects of PEG Pretreatment on EMS-induced Mutagenesis in Forage Oat（Avena sativa L.）Seeds

doi:10.11733/j.issn.1007-0435.2026.08.010

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (8): 2857-2865.DOI: 10.11733/j.issn.1007-0435.2026.08.010

Effects of PEG Pretreatment on EMS-induced Mutagenesis in Forage Oat（Avena sativa L.）Seeds

ZHONG Zhi-hua¹, LU Mao-jin¹, YANG Guo-dong¹, LIU Chao¹, WANG Rong¹, YAN Ming-ke¹, GUO Huan¹, MA Xiang², WANG Ya-fang¹

1. College of Grassland Agriculture, Northwest A&F University, Yangling, Shaanxi Province 712100, China;
2. Academy of Animal and Veterinary Science, Qinghai University, Xining, Qinghai Province 810016, China

Received:2026-02-23 Revised:2026-04-19 Published:2026-08-04

PEG前处理对饲用燕麦种子EMS诱变效应的影响

钟志花¹, 卢茂金¹, 杨国栋¹, 刘朝¹, 王蓉¹, 闫明科¹, 郭欢¹, 马祥², 王亚芳¹

1. 西北农林科技大学草业与草原学院, 陕西杨凌 712100;
2. 青海大学畜牧兽医科学院, 青海西宁 810016

通讯作者: 王亚芳,E-mail:yafang.wang@nwafu.edu.cn
作者简介:钟志花(2001-),女,汉族,青海海东人,硕士研究生,主要从事牧草种质资源与遗传育种研究,E-mail:zhihua@nwafu.edu.cn

Abstract

Abstract: To optimize the EMS-induced mutagenesis technology of forage oat （Avena sativa L.） and improve mutagenic efficiency， seeds of Oat cultivar ‘Qinghai 444’ and ‘Zhongtian No. 4’ were used as materials in this study. PEG osmotic priming was combined with EMS chemical mutagenesis to screen for optimum compound mutagenesis conditions for forage oat. Based on the germination characteristics and seedling traits of the M₁ generation in the two oat cultivars， the pretreatment conditions of 20% PEG for 3 h and 10% PEG for 6 h were found to promote seedling growth. The optimal EMS treatment conditions were 1% EMS for 16 h for ‘Zhongtian No.4’ and 0.75% EMS for 16 h for ‘Qinghai 444’. Through a comprehensive analysis of field agronomic traits at the milk stage in the M₂ generation after combined PEG and EMS treatments， it was found that 30% PEG for 3 h combined with the optimal EMS treatment produced the best results for developing germplasm with high-yield potential in both cultivars. Under these conditions， the mutagenesis efficiencies of the M₂ population were 2.40% for ‘Zhongtian No. 4’ and 1.26% for ‘Qinghai 444’， respectively， and a total of 22 germplasm lines with high-yield potential were obtained. These results provide a rich material basis for subsequent oat breeding.

Key words: Forage Oat, Mutagenesis, EMS, PEG, High yield, Germplasm

摘要： 为优化饲用燕麦（Avena sativa L.）诱变技术，提高饲用燕麦的诱变效率，本研究以‘青海444’和‘中天4号’种子为材料，采用PEG渗透调节前处理协同EMS化学诱变处理，筛选高效的饲用燕麦复合诱变条件。综合两个燕麦品种M₁代种子发芽特征及种苗特性，发现20% PEG 3 h和10% PEG 6 h 前处理条件能够促进幼苗生长；两个燕麦品种的最适EMS处理条件分别为1% EMS 16 h（‘中天4号’）和0.75% EMS 16 h（‘青海444’）。通过对PEG和EMS复合处理后M₂代乳熟期田间农艺性状综合分析，发现两个品种在30% PEG处理3 h协同最适EMS处理条件，在创制高产潜力种质方面效果最佳，其M₂代诱变效率分别为2.40%（‘中天4号’）和1.26%（‘青海444’），共获得具有高产潜力的种质材料22份，为后续燕麦育种提供了变异丰富的材料基础。

关键词: 饲用燕麦, 诱变, EMS, PEG, 高产, 种质

CLC Number:

S324

ZHONG Zhi-hua, LU Mao-jin, YANG Guo-dong, LIU Chao, WANG Rong, YAN Ming-ke, GUO Huan, MA Xiang, WANG Ya-fang. Effects of PEG Pretreatment on EMS-induced Mutagenesis in Forage Oat（Avena sativa L.）Seeds[J]. Acta Agrestia Sinica, 2026, 34(8): 2857-2865.

钟志花, 卢茂金, 杨国栋, 刘朝, 王蓉, 闫明科, 郭欢, 马祥, 王亚芳. PEG前处理对饲用燕麦种子EMS诱变效应的影响[J]. 草地学报, 2026, 34(8): 2857-2865.

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Effects of PEG Pretreatment on EMS-induced Mutagenesis in Forage Oat（Avena sativa L.）Seeds

PEG前处理对饲用燕麦种子EMS诱变效应的影响

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