Effects of Inoculating Feruloyl Esterase-Producing Lactobacillus reuteri on the Fermentation， Antioxidant and Digestibility of Alfalfa Baled Silage

doi:10.11733/j.issn.1007-0435.2026.08.035

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (8): 3131-3140.DOI: 10.11733/j.issn.1007-0435.2026.08.035

Effects of Inoculating Feruloyl Esterase-Producing Lactobacillus reuteri on the Fermentation， Antioxidant and Digestibility of Alfalfa Baled Silage

ZHANG Yan-wei¹, MAO Yu-qing¹, WANG Peng¹, TAO You-yan¹, GUO Xu-sheng², LI Fu-hou¹

1. College of Animal Sciences, Jilin University, Changchun, Jilin Province 130062, China;
2. College of Life Sciences, Lanzhou University, Lanzhou, Gansu Province 730000, China

Received:2025-11-17 Revised:2025-12-16 Published:2026-08-04

接种产阿魏酸酯酶罗伊氏乳杆菌对苜蓿裹包青贮发酵、抗氧化及消化率的影响

张艳威¹, 毛玉青¹, 王鹏¹, 陶有艳¹, 郭旭生², 李福厚¹

1. 吉林大学动物科学学院, 吉林长春 130062;
2. 兰州大学生命科学学院, 甘肃兰州 730000

通讯作者: 李福厚,E-mail:lifh@jlu.edu.cn
作者简介:张艳威(2002-),男,汉族,山东聊城人,硕士研究生,主要从事饲草生产加工与反刍动物健康研究,E-mail:19861208291@163.com
基金资助:
国家自然科学基金项目（32301493）资助

Abstract

Abstract: This study investigated the effects of inoculation with feruloyl esterase-producing lactic acid bacteria Lactobacillus reuteri A4-2 on the fermentation quality， antioxidant status， and in vitro digestibility of baled alfalfa silage. Chopped fresh alfalfa （Medicago sativa L.） at the early to full-blossom flowering stage was randomly divided into three groups： CON （without inoculant）， YM13 （inoculated with non-feruloyl esterase-producing L. reuteri YM13）， and A4-2 （inoculated with L. reuteri A4-2）. Samples were collected after 20， 40， and 60 d of ensiling to determine fermentation characteristics， free ferulic acid content， antioxidant enzyme activities， chemical composition， and in vitro dry matter digestibility. Compared with the CON and YM13， inoculation with L. reuteri A4-2 significantly reduced pH but increased lactic and acetic acid contents （P<0.05）. Both inoculating L. reuteri YM13 and L. reuteri A4-2 in silage showed a higher dry matter content and lower ammonia nitrogen/total nitrogen than CON （P<0.05）. Inoculation with L. reuteri A4-2 in alfalfa silage markedly enhanced the levels of free ferulic acid， superoxide dismutase （SOD）， catalase （CAT）， glutathione peroxidase （GPX） activities， and total antioxidant capacity （T-AOC）（P<0.05） relative to CON and YM13 during ensiling. In addition， inoculation with L. reuteri A4-2 effectively decreased neutral detergent fiber （NDF） and acid detergent fiber （ADF） contents， thereby improving in vitro DM digestibility compared with the other treatments （P<0.05）. Overall， inoculation with L. reuteri A4-2 not only improved the fermentation quality of silage， but also enhanced the antioxidant capacity， reduced its fiber content， and improved its in vitro DM digestibility compared with the CON and YM13 groups， demonstrating significant application advantages.

Key words: Alfalfa, Silage, Ferulic Acid, Antioxidant, Digestibility

摘要： 为探讨接种产阿魏酸酯酶的罗伊氏乳杆菌A4-2（Lactobacillus reuteri A4-2）对裹包苜蓿青贮饲料发酵品质、抗氧化性能及体外消化率的影响，本试验以切碎的初花期至盛花期紫花苜蓿（Medicago sativa L.）为原料，随机分为3组进行裹包青贮：CON组（不接种乳酸菌）、YM13组（接种不产阿魏酸酯酶的L. reuteri YM13）和A4-2组（接种产阿魏酸酯酶的L.reuteri A4-2）。青贮至20，40和60 d时测定其发酵特性、游离阿魏酸含量、抗氧化酶活性、化学成分及体外干物质消化率。结果表明：与CON和YM13组相比，接种L. reuteri A4-2能显著降低青贮饲料的pH值并提高乳酸和乙酸含量（P<0.05）。接种L. reuteri YM13和L. reuteri A4-2均较CON提高了青贮饲料中干物质含量，并降低了氨态氮/总氮（P<0.05）。A4-2处理组的游离阿魏酸含量、超氧化物歧化酶（Superoxide dismutase，SOD）、过氧化氢酶（Catalase，CAT）、谷胱甘肽过氧化物酶（Glutathione peroxidase，GPX）活性及总抗氧化能力（Total antioxidant capacity，T-AOC）均显著高于其他两组（P<0.05）。此外，接种L. reuteri A4-2显著降低了青贮饲料的中性洗涤纤维（Neutral detergent fiber，NDF）和酸性洗涤纤维（Acid detergent fiber，ADF）含量，并最终使其体外干物质消化率显著高于其他两组（P<0.05）。综上，接种L. reuteri A4-2不仅可改善裹包苜蓿青贮的发酵品质，而且相较于CON和YM13处理组，还能增强青贮饲料的抗氧化能力，降低纤维含量并提高消化率，具有显著的应用优势。

关键词: 苜蓿, 青贮, 阿魏酸, 抗氧化, 消化率

CLC Number:

S816.53

ZHANG Yan-wei, MAO Yu-qing, WANG Peng, TAO You-yan, GUO Xu-sheng, LI Fu-hou. Effects of Inoculating Feruloyl Esterase-Producing Lactobacillus reuteri on the Fermentation， Antioxidant and Digestibility of Alfalfa Baled Silage[J]. Acta Agrestia Sinica, 2026, 34(8): 3131-3140.

张艳威, 毛玉青, 王鹏, 陶有艳, 郭旭生, 李福厚. 接种产阿魏酸酯酶罗伊氏乳杆菌对苜蓿裹包青贮发酵、抗氧化及消化率的影响[J]. 草地学报, 2026, 34(8): 3131-3140.

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Effects of Inoculating Feruloyl Esterase-Producing Lactobacillus reuteri on the Fermentation， Antioxidant and Digestibility of Alfalfa Baled Silage

接种产阿魏酸酯酶罗伊氏乳杆菌对苜蓿裹包青贮发酵、抗氧化及消化率的影响

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