Plant Regeneration of Leymus chinensis Via in Vitro Culture

doi:10.11733/j.issn.1007-0435.2002.03.009

›› 2002, Vol. 10 ›› Issue (3): 198-202.DOI: 10.11733/j.issn.1007-0435.2002.03.009

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Plant Regeneration of Leymus chinensis Via in Vitro Culture

LIU Gong-she, WANG En-hua, LIU Jie, QI Dong-mei, LI Fang-fang

Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China

Received:2002-01-31 Revised:2002-03-28 Online:2002-08-15 Published:2002-08-15

羊草幼穗离体培养诱导植株再生的研究

刘公社, 汪恩华, 刘杰, 齐冬梅, 李芳芳

中国科学院植物研究所, 北京, 100093

作者简介:刘公社(1958- ),男,陕西人,1986年在法国获生物学博士学位.1986-1988年回国从事博士后研究工作,现为中国科学院植物研究所首席研究员,研究方向为生物工程及其在持续农业上的应用.发表学术论文72篇,编著3部.
基金资助:
Supported by Key Project of the Chinese Academy of Science (KSCX1-08-03) and State Key Project of the Ministry of Science and Technology (2001BA707B02)

Abstract

Abstract: The regenerated plants were obtained from Leymus chinensis for the first time through tissue culture.Immature-base explants were cultured on Murashige and Skoog’s basal medium(MS) supplemented with 2 mg/L 2,4-D after surface sterilized with 0.1% HgCl₂ to induce callus at 25℃ constant temperature.After moved to MS medium containing 1mg/L 2,4-D for two times,the compact calluses were selected and transferred to MS basal medium containing 1.0mg/L Kt and 0.5mg/L NAA,which differentiated into shoot-buds. The shoot-buds appeared and regenerated new roots,finally developed plantlets on 2,4-D-free basal medium. Regenerated plants growth well when transferred into greenhouse.A comparative study of different explants,in this study,revealed that only could the callus from immature stachys differentiate into regenerated plants. The differentiate potential of regenerated plants has close related to the genotype and external hormone.

Key words: Leymus chinensis, Immature stachys, Explant, Plant regeneration

摘要： 采用离体培养方法首次获得羊草体细胞再生植株。其方法要点是:取羊草幼穗为外植体,经0.1%HgCl₂溶液表面消毒后,接种到含2mg/L2,4-D的MS培养基上,置于恒温25℃条件下诱导愈伤组织。在加有1mg/L2,4-DMS培养基上继代2次后,转移到含1.0mg/LKT和0.5mg/LNAA的MS培养基上分化培养得到再生芽。在无激素的基本培养基上获得了生根的试管苗,试管苗移栽到温室后生长正常。研究结果还表明,尽管从羊草叶片、幼穗和成熟胚在同样培养条件下均能诱导出愈伤组织,但只有幼穗愈伤组织能够继续分化出再生植株。羊草试管苗的分化因基因型和外源激素的不同而异。

关键词: 羊草(Leymus chinensis), 幼穗, 外殖体, 再生植株

CLC Number:

Q813.1

LIU Gong-she, WANG En-hua, LIU Jie, QI Dong-mei, LI Fang-fang. Plant Regeneration of Leymus chinensis Via in Vitro Culture[J]. , 2002, 10(3): 198-202.

刘公社, 汪恩华, 刘杰, 齐冬梅, 李芳芳. 羊草幼穗离体培养诱导植株再生的研究[J]. , 2002, 10(3): 198-202.

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Plant Regeneration of Leymus chinensis Via in Vitro Culture

羊草幼穗离体培养诱导植株再生的研究

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