Study on the Ice Crystal Modification of Rhizome Protein in Elytrigia repens(L.) Desv.

doi:10.11733/j.issn.1007-0435.2009.03.013

›› 2009, Vol. 17 ›› Issue (3): 333-336.DOI: 10.11733/j.issn.1007-0435.2009.03.013

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Study on the Ice Crystal Modification of Rhizome Protein in Elytrigia repens(L.) Desv.

GONG Shu-fang¹, YANG Tao¹, LI Yan², CHE Dai-di¹

1. College of Horticulture, Northeast Agricultural University, Harbin, Heilongjiang Province 150030, China;
2. Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang Province 150086, China

Received:2008-03-27 Revised:2009-03-03 Online:2009-06-15 Published:2009-06-15

偃麦草根茎蛋白质修饰冰晶的研究

龚束芳¹, 杨涛¹, 李岩², 车代弟¹

1. 东北农业大学园艺学院, 哈尔滨, 150030;
2. 黑龙江省农科院, 哈尔滨, 150086

通讯作者: 车代弟,E-mail:daidiche@yahoo.com.cn
作者简介:龚束芳(1971- ),女,博士,副教授,研究方向为草坪逆境生理与品种选育,E-mail:shufanggong@yahoo.com.cn
基金资助:
黑龙江省教育厅项目(11511037)

Abstract

Abstract: The kinds of the rhizome soluble protein and the conformation difference of single ice crystal of wild Elytrigia repens(L.) Desv.from Harbin areas were comparatively studied after chilled treatment taking the plants growing in the 10℃ as the CK.The result show that the rhizome protein had a deletion of two protein bands with molecular weight of 33 and 13 kda and an addition of two protein bands with molecular weight of 53 and 35 kda after chilled treatment.The protein of 53 and 35 kda appeared spindly or conoid ice crystal indicating a high antifreeze activity.

Key words: Elytrigia repens(L.) Desv., Antifreeze activity, Protein, Ice crystal

摘要： 以哈尔滨地区野生偃麦草(Elytrzgia repens(L.)Desv.)为研究对象,以10℃下生长的植株为对照,比较冷冻处理后,偃麦草根茎可溶性蛋白质种类及修饰单体冰晶形态的差异。结果显示:冷冻处理后缺失两条蛋白质带,分子量分别为33和13 Kda,同时,增加两条新带,分子量分别约为53和35 Kda,而且均有纺锤状或锥状的单体冰晶出现,表明其具有极强抗冻活性。

关键词: 偃麦草, 抗冻性, 蛋白质, 冰晶

CLC Number:

S812

GONG Shu-fang, YANG Tao, LI Yan, CHE Dai-di. Study on the Ice Crystal Modification of Rhizome Protein in Elytrigia repens(L.) Desv.[J]. , 2009, 17(3): 333-336.

龚束芳, 杨涛, 李岩, 车代弟. 偃麦草根茎蛋白质修饰冰晶的研究[J]. , 2009, 17(3): 333-336.

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Study on the Ice Crystal Modification of Rhizome Protein in Elytrigia repens(L.) Desv.

偃麦草根茎蛋白质修饰冰晶的研究

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