Isolation and Identification of Lactic Acid Bacteria from Silage and Filtering of Excellent Strains

doi:10.11733/j.issn.1007-0435.2011.01.024

›› 2011, Vol. 19 ›› Issue (1): 137-141.DOI: 10.11733/j.issn.1007-0435.2011.01.024

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Isolation and Identification of Lactic Acid Bacteria from Silage and Filtering of Excellent Strains

ZHANG Hui-jie¹, YU Zhu², WANG Lin¹, CAI Yi-min³, LI Feng⁴, TAO Ya⁴, SUN Qi-zhong⁴

1. Graduate School, Chinese Academy of Agricultural Science, Beijing 100081, China;
2. China Agriculture University, Beijing 100094, China;
3. National Institute of Livestock and Grassland Science, Nasushiobara 329-2793, Japan;
4. Grassland Research Institute, CAAS, Hohhot, Inner Mongolia 010010, China

Received:2010-11-10 Revised:2010-12-01 Online:2011-02-15 Published:2011-02-15

青贮饲料中乳酸菌的分离鉴定及优良菌株筛选

张慧杰¹, 玉柱², 王林¹, 蔡义民³, 李峰⁴, 陶雅⁴, 孙启忠⁴

1. 中国农业科学院研究生院, 北京, 100081;
2. 中国农业大学草地研究所, 北京, 100094;
3. 日本畜产草地研究所, 枥木县那须郡, 日本, 329-2793;
4. 中国农业科学院草原研究所, 内蒙古, 呼和浩特, 010010

通讯作者: 孙启忠,E-mail:sunqz@126.com
作者简介:张慧杰(1986- ),女,内蒙古锡林郭勒盟人,硕士研究生,研究方向为牧草加工利用,E-mail:zhj439389793@yahoo.com.cn
基金资助:
现代农业产业技术体系建设专项资金;中央级公益性科研院所基本科研业务费专项资金;公益性行业(农业)科研专项经费项目(nyhyzx07-022);“948”项目(2010-G15);内蒙古自治区自然科学基金项目(2010MS0402)资助

Abstract

Abstract: To study the taxonomic,morphologic,physiological and biochemical characteristics of lactic acid bacteria(LAB) in silage,five strains of LAB were isolated from alfalfa(Medicago sativa L.)and maize(Zea mays L.)silage.The 16S rRNA sequence analysis indicate that two strains are Pediococcus pentosaceus(GI7,GI24),and the other three are Pediococcus acidilactici,Lactobacillus curvatus and Lactobacillus plantarum(GI11,GI44,GI62).Each is homofermentative lactic acid bacteria and can grow well or weakly at 5,10,40,45℃,and with pH3.0~8.0,3.0% and 6.5% NaCl except that GI44 does not grow at pH3.0,4.0,8.0.Through measuring the rate of lactic acid production and the growth curve,GI62 was selected as eximious strain used for preparing lactic acid bacteria additive due to its rapid acid-production and growth speed.

Key words: Silage, Lactic acid bacteria, Eximious strain

摘要： 为研究青贮饲料中乳酸菌的分类及形态学与生理生化特性,从苜蓿(Medicago sativa L.)及玉米(Zea maysL.)青贮饲料中分离得到5株乳酸菌。经16S rRNA序列分析,2株(GI7,GI24)为乳酸片球菌(Pediococcus acidilactici),其余3株(GI11,GI44,GI62)分别为戊糖片球菌(Pediococcus pentosaceus)、弯曲乳杆菌(Lactobacillus curvatus)和植物乳杆菌(Lactobacillus plantarum);5株菌均为同型发酵乳酸菌,除弯曲乳杆菌GI44在pH3.0,4.0,8.0等条件下不能生长外,其余菌株均可在5,10,40,45℃等不同温度条件下、pH3.0~8.0等环境下以及3.0%和6.5%NaCl溶液中良好生长或微弱生长。同时,通过产酸速率和生长曲线等指标筛选出产酸较快、生长迅速的GI62可作为制备乳酸菌青贮饲料添加剂的优良菌株。

关键词: 青贮, 乳酸菌, 优良菌株

CLC Number:

S816.53

ZHANG Hui-jie, YU Zhu, WANG Lin, CAI Yi-min, LI Feng, TAO Ya, SUN Qi-zhong. Isolation and Identification of Lactic Acid Bacteria from Silage and Filtering of Excellent Strains[J]. , 2011, 19(1): 137-141.

张慧杰, 玉柱, 王林, 蔡义民, 李峰, 陶雅, 孙启忠. 青贮饲料中乳酸菌的分离鉴定及优良菌株筛选[J]. , 2011, 19(1): 137-141.

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Isolation and Identification of Lactic Acid Bacteria from Silage and Filtering of Excellent Strains

青贮饲料中乳酸菌的分离鉴定及优良菌株筛选

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