SRAP and RAPD Analysis of Elymus nutans Griseb.Germplasm from the Qinghai-Tibetan Plateau and Xinjiang

doi:10.11733/j.issn.1007-0435.2011.02.023

›› 2011, Vol. 19 ›› Issue (2): 306-316.DOI: 10.11733/j.issn.1007-0435.2011.02.023

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SRAP and RAPD Analysis of Elymus nutans Griseb.Germplasm from the Qinghai-Tibetan Plateau and Xinjiang

MIAO Jia-min¹, ZHANG Xin-quan¹, CHEN Zhi-hua², ZHONG Jin-cheng², CHEN Shi-yong¹, MA Xiao¹, BAI Shi-qie³

1. Department of grassland science, Sichuan Agricultural University, Ya’an, Sichuan Province 625014, China;
2. College of life science and technology, Southwest University for Nationalities, Chengdu, Sichuan Province 610041, China;
3. Academy of Grassland Science, Chengdu, Sichuan Province 611731, China

Received:2010-10-12 Revised:2011-03-08 Online:2011-04-15 Published:2011-04-15

青藏高原和新疆地区垂穗披碱草种质的SRAP及RAPD分析

苗佳敏¹, 张新全¹, 陈智华², 钟金城², 陈仕勇¹, 马啸¹, 白史且³

1. 四川农业大学草业科学系, 四川, 雅安, 625014;
2. 西南民族大学生命科学与技术学院, 四川, 成都, 610041;
3. 四川省草原科学研究院, 四川, 成都, 611731

通讯作者: 张新全,E-mail:zhangxq@sicau.edu.cn
作者简介:苗佳敏(1982- ),女,河南新乡人,博士,研究方向为牧草育种与种质资源研究,E-mail:mjmgrass@163.com
基金资助:
国家重点基础研究发展计划973项目(2007CB108907);国家自然科学基金项目(NSFC31072077)资助

Abstract

Abstract: The diversity and relationships among 64 E.nutans accessions were analyzed using sequence-related amplified polymorphism(SRAP) and random amplified polymorphic DNA(RAPD) molecular markers to measure the genetic variability and diversity.Results show high genetic diversity in E.nutans(PPB=85.86%,90.39%) by using both molecular markers.Cluster analysis indicates the accessions from similar origins frequently clustered into one group.The analysis of molecular variance(AMOVA) by using both molecular markers shows that the proportion of variance either within or among geographical groups is 48.23%,39.87% and 51.77%,60.13%,respectively.Additionally,analysis of genetic variation using SRAP markers is more efficient than using RAPD markers for E.nutans.Distinct genetic differentiation accession is revealed between Qinghai-Tibetan Plateau and Xinjiang.Eco-geographical conditions such as climate and mountain ranges as well as mating system may be key factors of genetic variations.These results provide management considerations for breeding programs and germplasm collections as well as for the overall conservation of E.nutans.

Key words: Elymus nutans, SRAP, RAPD, Genetic variation, AMOVA

摘要： 使用SRAP和RAPD标记对采集自我国青藏高原和新疆地区的64份垂穗披碱草(Elymus nutans Griseb.)进行遗传多样性和亲缘关系分析,并测其遗传变异和各地理类群的遗传多样性水平。结果表明:2种标记都显示供试材料具有较高的遗传多样性水平(PPB=85.86%,90.39%),且新疆地区材料的遗传多样性水平高于青藏高原地区,它们分别得到相似但并不完全相同的聚类图,相似生态地理环境的材料可以聚为一类;2种标记的分子方差分析(AMOVA)揭示了地理类群内部和地理类群间分别有48.23%,39.87%和51.77%,60.13%的变异;通过2种标记的比较分析,SRAP能更高效地对垂穗披碱草种质进行遗传变异分析;青藏高原和新疆地区的材料存在明显的遗传分化,气候和山脉等生态地理条件以及繁育系统等可能是使材料发生遗传变异的重要因素。这些结果将为垂穗披碱草种质的育种以及种质资源的收集和保存提供基础依据。

关键词: 垂穗披碱草, SRAP, RAPD, 遗传变异, 分子方差分析

CLC Number:

Q346

MIAO Jia-min, ZHANG Xin-quan, CHEN Zhi-hua, ZHONG Jin-cheng, CHEN Shi-yong, MA Xiao, BAI Shi-qie. SRAP and RAPD Analysis of Elymus nutans Griseb.Germplasm from the Qinghai-Tibetan Plateau and Xinjiang[J]. , 2011, 19(2): 306-316.

苗佳敏, 张新全, 陈智华, 钟金城, 陈仕勇, 马啸, 白史且. 青藏高原和新疆地区垂穗披碱草种质的SRAP及RAPD分析[J]. , 2011, 19(2): 306-316.

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SRAP and RAPD Analysis of Elymus nutans Griseb.Germplasm from the Qinghai-Tibetan Plateau and Xinjiang

青藏高原和新疆地区垂穗披碱草种质的SRAP及RAPD分析

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