Study on the Salt Tolerance of Transgenic Tall Fescue

doi:10.11733/j.issn.1007-0435.2013.06.017

›› 2013, Vol. 21 ›› Issue (6): 1144-1147.DOI: 10.11733/j.issn.1007-0435.2013.06.017

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Study on the Salt Tolerance of Transgenic Tall Fescue

MA Dong-mei¹, JIN Feng-xia¹, XU Xing², LI Hui-wen², WEI Rui², GUO Ling-na²

1. Agricultural College, Ningxia University, Yinchuan, Ningxia 750021, China;
2. College of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China

Received:2013-05-07 Revised:2013-09-26 Online:2013-12-15 Published:2013-11-27

高羊茅耐盐转基因抗性苗的耐盐性研究

麻冬梅¹, 金凤霞¹, 许兴², 李会文², 魏睿², 郭伶娜²

1. 宁夏大学农学院, 宁夏银川 750021;
2. 宁夏大学生命科学学院, 宁夏银川 750021

作者简介:麻冬梅（1978-），女，宁夏银川人，博士，讲师，主要从事牧草育种与植物抗逆分子生物学研究，E-mail：mdm319@tom.com
基金资助:
国家973项目“鉴定由调控网络重要节点构成的调控单元，并进行耐盐碱作物分子育种设计课题”（2012CB114204）；“十一五”国家科技支撑计划资助项目“荒漠草原区农牧复合生态系统构建与可持续利用技术集成试验与示范”（2011BAC07B03）；宁夏农发办项目（NTKJ-2013-09（1））资助

Abstract

Abstract: Transgenic ‘Arid 3’ plants (Festuca arundinacea) under fully controlled conditions treated with the different concentrations of NaCl for 14 days were used as test materials in this study. The physiological indexes related to salt tolerance, such as proline (Pro) and malondialdehyde (MDA) contents, electrical conductivity rate, superoxide dismutase (SOD) and catalase (POD) activities were measured. Results showed that Pro contents, SOD and POD activities of transgenic seedling were significant higher than that of control(P<0.05), whereas the relative conductivity and MDA content of transgenic seedling were lower than that of control when NaCl concentration was above 1.5%(P<0.05). These results suggest that the salt tolerance of the transgenic plant is obviously enhanced.

Key words: Transgene, Tall fescue, Salt tolerance

摘要： 以转基因高羊茅爱瑞三号（Festuca arundinacea ‘Arid 3’）植株为材料，采用不同梯度的NaCl进行盐胁迫处理14 d后，通过对其脯氨酸（Pro）含量、丙二醛（MDA）含量、电导率及超氧化物歧化酶（SOD）活性和过氧化物酶（POD）活性等抗旱耐盐性生理生化指标的测定，进行了耐盐性鉴定研究。结果表明：当NaCl浓度大于1.5%，在同一盐浓度下转基因植株的Pro含量、SOD和POD活性均显著高于对照植株（P<0.05），而相对电导率和MDA含量低于对照植株（P<0.05）。这表明转基因植株的耐盐性得到了显著增强。

关键词: 转基因, 高羊茅, 耐盐性

CLC Number:

MA Dong-mei, JIN Feng-xia, XU Xing, LI Hui-wen, WEI Rui, GUO Ling-na. Study on the Salt Tolerance of Transgenic Tall Fescue[J]. , 2013, 21(6): 1144-1147.

麻冬梅, 金凤霞, 许兴, 李会文, 魏睿, 郭伶娜. 高羊茅耐盐转基因抗性苗的耐盐性研究[J]. , 2013, 21(6): 1144-1147.

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References

[1] 石元春. 盐碱土改良-诊断、管理、改良[M]. 北京:农业出版社,1986:122
[2] 汤巧香,蒋桂香.高羊茅草坪草的耐盐性鉴定研究[J].山东林业科技,2009(3):37-38
[3] Van H,Hutchinson R J,McDonald G,et al. The role of biotechnology in perennial grass improvement for temperate pastures[J]. New Zealand Journal of Agricultural Research,1994,37(3):427-438
[4] Guenzi A C,Mornhinweg D W,Johnson B, et al. Genetic analysis of a grass dwarf mutation induced by wheat callus culture[J]. Theoretical and Applied Genetics,1992,84(7):952-957
[5] Belanger F C, Laramore C, Bonos S, et al. Development of improved turfgrass with herbicide resistance and enhanced disease resistance through transformation[C]//Fate of Turfgrass Chemicals and Pest Management Approaches. ACS Book,2000:325-329
[6] 王艳,李建龙,潘永年,等. 草坪草转基因育种研究进展[J].草原与草坪,2007(4):13-17
[7] 吴錡,胡鸢雷,倪挺,等. 转基因草坪草的研究概况与展望[J].草业科学,2004,21(1):29-34
[8] 邹琦,翟伟菁.植物生理学实验指导[M].北京:中国农业出版社,2000:72-75
[9] 李合生. 植物生理生化实验原理和技术[M].北京:高等教育出版社,2006:134-137
[10] 刘友良,毛才良,王良驹,等. 植物耐盐性研究进展[J]. 植物生理学通讯,1987(4):1-7
[11] 朱诚,曾广文. 4PU23对水稻叶片衰老与活性氧代谢的影响[J]. 浙江大学学报:农业与生命科学版,2000,26(5):483-488
[12] 韩瑞宏,毛凯,干友民,等. 干旱对草坪草的影响[J].草原与草坪,2003(1):8-10
[13] 杨富裕,周禾. 草坪草抗盐性研究进展[J].草原与草坪,2001(1):10-13
[14] 彭志红,彭克勤,胡家金,等. 渗透胁迫下植物脯氨酸积累的研究进展[J].中国农学通报,2002,18(4):80-83
[15] 刘德立,禹邦超,余世明,等. 超氧物歧化酶(SOD)与植物抗逆性的关系[J]. 华中师范大学学报:自然科学版,1993,27(1):83-85

Study on the Salt Tolerance of Transgenic Tall Fescue

高羊茅耐盐转基因抗性苗的耐盐性研究

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