Physiological and Biochemical Responses of Turf-type Tall Fescue Seedlings to Substrate Contaminated by Lead

doi:10.11733/j.issn.1007-0435.2015.01.015

Acta Agrestia Sinica ›› 2015, Vol. 23 ›› Issue (1): 95-100.DOI: 10.11733/j.issn.1007-0435.2015.01.015

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Physiological and Biochemical Responses of Turf-type Tall Fescue Seedlings to Substrate Contaminated by Lead

LIU Hui-qin^1,2, SHEN Gao-feng³, LIU Yi², LIU Hui-ping¹, HAN Ju-cai¹

1. Agronomy College, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China;
2. College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300384, China;
3. Department of agronomy, Tianjin Agricultural University, Tianjin 300384, China

Received:2014-03-17 Revised:2014-05-23 Online:2015-02-15 Published:2015-01-31

草坪型高羊茅幼苗对基质铅污染的生理生化响应

刘慧芹^1,2, 沈高峰³, 刘峄², 刘慧平¹, 韩巨才¹

1. 山西农业大学农学院, 山西太谷 030801;
2. 天津农学院园艺园林学院, 天津 300384;
3. 天津农学院农学系, 天津 300384

通讯作者: 韩巨才
作者简介:刘慧芹(1973-),女,山西太原人,副教授,博士研究生,主要从事逆境与植物生理学及农药污染研究,E-mail:wjxlhq@126.com
基金资助:
山西省财政支持农业科技成果转化基金项目(SCZZNCGZH201304)(CZZNCGZH201403);山西省科技基础条件平台建设项目(2014091003-0102)

Abstract

Abstract:

In order to explore phytoremediation and physiological response of plant to heavy metal, physiological and biochemical changes of tall fescue seedlings were comparatively analyzed when they were contaminated by lead at different times and concentrations. The results showed that superoxide dismutase (SOD) and peroxidase (POD) activities in tall fescue increased initially and then decreased overall with the extension of stress time at three tested concentrations. Change of POD activity is slower. It indicated that lead can rapidly induce tall fescue protecting enzyme activity at low concentrations or in a short period. Malondialdehyde (MDA) contents were positively correlated with stress time and stress concentrations, which indicated that the peroxidation of membrane lipid was strengthened with the increases of stress concentration and time. Changes of chlorophyll total concentration increased first and then decreased at low concentrations (500 and 1000 mg·L^-1), and continued to decline at high concentration. It indicated that tall fescue could adapt low lead stress by increasing chlorophyll, and might lose the regulation at high concentrations. Proline content decreased initially and then increased with the extension of stress time at 500 and 1000 mg·L^-1, however, it was opposite at 1500 mg·L^-1. The proline contents of tall fescue at all treatments were higher than controls, indicating that osmotic adjustment ability was enhanced after lead stress. Changes of soluble sugar content were "down-up-down" with the extension of stress times at three stress concentrations, and the soluble sugar contents of tall fescue at all treatments were significantly lower than controls in the end. The changes of physiological characteristics in the process of lead stress indicate that tall fescue is active adaptation and resistance to the lead stress of low concentration, endurance and injury under the stress of high concentration.

Key words: Lead contamination, Festuca arundinacea, Substrate, Physiology and biochemistry effects

摘要：

为探索植物对重金属的修复作用以及自身对重金属污染的生理效应,以基质盆栽高羊茅(Festuca arundinacea)幼苗为试验材料,比较分析了不同铅胁迫下,高羊茅的生理生化效应.结果表明:随着胁迫时间的延长,不同铅浓度下的高羊茅叶片中超氧化物歧化酶(SOD)和过氧化物酶(POD)活性整体变化为先升后降,POD变化趋势较为缓慢,表明低浓度或短时间内铅可快速诱导高羊茅保护酶活性.叶绿素总量变化为低浓度(500,1000 mg·L^-1)下先增加后下降,高浓度下则为持续下降,表明高羊茅可能通过增加叶绿素来适应低铅胁迫,而高浓度下则失去了这种调节能力.3个铅浓度下的丙二醛(MDA)含量与胁迫时间及胁迫浓度均为正相关,表明随着胁迫浓度和时间的增加,细胞膜脂过氧化作用加强.脯氨酸含量在500,1000 mg·L^-1铅浓度下随时间增加为先降后升,1500 mg·L^-1浓度下则相反,但均高于对照,表明经铅胁迫后细胞的渗透调节能力增强.3个浓度下的可溶性糖含量变化均随时间表现出降-升-降,最终显著降低,整个变化过程低于对照.因此在铅胁迫过程中,这些生理特性的变化表明在低铅胁迫时高羊茅表现为积极适应和抵抗,而在高铅胁迫下则表现为忍耐和受损.

关键词: 铅污染, 高羊茅, 基质, 生理生化效应

CLC Number:

QS688
X171

LIU Hui-qin, SHEN Gao-feng, LIU Yi, LIU Hui-ping, HAN Ju-cai. Physiological and Biochemical Responses of Turf-type Tall Fescue Seedlings to Substrate Contaminated by Lead[J]. Acta Agrestia Sinica, 2015, 23(1): 95-100.

刘慧芹, 沈高峰, 刘峄, 刘慧平, 韩巨才. 草坪型高羊茅幼苗对基质铅污染的生理生化响应[J]. 草地学报, 2015, 23(1): 95-100.

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Physiological and Biochemical Responses of Turf-type Tall Fescue Seedlings to Substrate Contaminated by Lead

草坪型高羊茅幼苗对基质铅污染的生理生化响应

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