Effect of PEG Priming on Oxidation Resistance of Aged Oat Seeds

doi:10.11733/j.issn.1007-0435.2016.05.001

Acta Agrestia Sinica ›› 2016, Vol. 24 ›› Issue (5): 933-938.DOI: 10.11733/j.issn.1007-0435.2016.05.001

Effect of PEG Priming on Oxidation Resistance of Aged Oat Seeds

XIA Fang-shan^1,2, MAO Pei-sheng², WANG Ming-ya², YAN Hui-fang², HOU Long-yu², CHEN Ling-ling², ZHU Yan-qiao², WANG Hui²

1. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China;
2. College of Animal Science and Technology, China Agricultural University, Beijing Key Laboratory of Grassland Science, Beijing 100193, China

Received:2015-10-12 Revised:2016-03-16 Online:2016-10-15 Published:2017-01-13

PEG引发对老化燕麦种子抗氧化性能的影响

夏方山^1,2, 毛培胜², 王明亚², 闫慧芳², 侯龙鱼², 陈玲玲², 朱艳乔², 汪辉²

1. 山西农业大学动物科技学院, 山西太谷 030801;
2. 中国农业大学动物科技学院草业科学北京市重点实验室, 北京 100193

通讯作者: 毛培胜
作者简介:夏方山(1983-),男,山东安丘人,博士,研究方向为草类植物育种与种子科学,E-mail:dqlxfs8583@163.com
基金资助:
博士点基金项目（20110008110003）；国家“十二五”科技支撑课题“优质多抗牧草新品种选育与良种繁育关键技术研究与示范（2011BAD17B01-02）”资助

Abstract

Abstract:

This study was designed to analyze the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and malondialdehyde (MDA) content in ultra-dried (4% moisture content) oat (Avena sativa) seeds. The seeds were deteriorated at 45℃ for 48 d, and then primed with different concentrations of PEG-6000 (0, -0.3, -0.6, -0.9 and -1.2 Mpa) for 0 (CK), 3 and 6 h. This study was to explore the effect of PEG priming on physiological responses of aged ultra-dry oat seeds. The results showed that the activities of SOD, CAT and POD decreased in aged oat seeds primed with PEG at the concentration of 0 and -0.3 Mpa, leading to an increase in MDA content and decrease in germination percentage. However, the opposite trend was observed after priming with high concentration of PEG (-0.9 and -1.2 Mpa). This indicated that PEG priming repaired the damage of ageing and prevented the imbibition damage in ultra-dried oat seeds by improving antioxidant capacity.

Key words: Oat, Priming, Polyethylene glycol, Seed ageing, Ultra-dry

摘要：

试验以45℃老化48 d的超干（含水量4%）燕麦（Avena sativa）种子为材料，通过不同浓度PEG-6000（0，-0.3，-0.6，-0.9和-1.2 Mpa）引发0（CK），3和6 h后，分析其超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、过氧化物酶（POD）活性及丙二醛（MDA）含量的变化，以探讨PEG引发对老化的超干燕麦种子抗氧化性能的影响。结果表明：浓度为0和-0.3 Mpa的PEG引发会降低SOD，CAT和POD的活性，导致MDA含量增加，从而降低老化燕麦种子发芽率；而高浓度（-0.9和-1.2 Mpa）的PEG引发则相反。说明适当浓度的PEG引发主要通过提高超干燕麦种子的抗氧化能力来修复其老化损伤，并避免其吸胀损伤。

关键词: 燕麦, 引发, 聚乙二醇, 种子老化, 超干

CLC Number:

S512.6

XIA Fang-shan, MAO Pei-sheng, WANG Ming-ya, YAN Hui-fang, HOU Long-yu, CHEN Ling-ling, ZHU Yan-qiao, WANG Hui. Effect of PEG Priming on Oxidation Resistance of Aged Oat Seeds[J]. Acta Agrestia Sinica, 2016, 24(5): 933-938.

夏方山, 毛培胜, 王明亚, 闫慧芳, 侯龙鱼, 陈玲玲, 朱艳乔, 汪辉. PEG引发对老化燕麦种子抗氧化性能的影响[J]. 草地学报, 2016, 24(5): 933-938.

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Effect of PEG Priming on Oxidation Resistance of Aged Oat Seeds

PEG引发对老化燕麦种子抗氧化性能的影响

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