Interaction Effect of Soil Drought and Strong Light on Photosynthetic Performance of Kobresia Pygmaea in Qinghai-Tibetan Plateau

doi:10.11733/j.issn.1007-0435.2017.04.006

Acta Agrestia Sinica ›› 2017, Vol. 25 ›› Issue (4): 724-731,748.DOI: 10.11733/j.issn.1007-0435.2017.04.006

Interaction Effect of Soil Drought and Strong Light on Photosynthetic Performance of Kobresia Pygmaea in Qinghai-Tibetan Plateau

SHI Sheng-bo^1,3, LI Tian-cai¹, WANG Wei², DE Ke-jia², XU Xian-ying³, WANG Qi³, MA Jian-ping³, LI Ai-de³, KANG Cai-zhou³

1. Key Laboratory of Adaptation and Evolution of Plateau Biology/Key Laboratory of Crop Molecular Breeding of Qinghai Province/Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai Province 810001, China;
2. Qinghai Academy of Animal Science and Veterinary Medicine/Qinghai University, Xining, Qinghai Province 810016, China;
3. Research Center of Engineer Technique for Desert Plants in Gansu/Gansu Desert Control Research Institute, Lanzhou, Gansu Province 730070, China

Received:2016-06-20 Revised:2017-06-15 Online:2017-08-15 Published:2017-11-01

土壤干旱和强光交互作用对青藏高原高山嵩草光合功能的影响

师生波^1,3, 李天才¹, 王伟², 德科加², 徐先英³, 王祺³, 马剑平³, 李爱德³, 康才周³

1. 中国科学院西北高原生物研究所/高原生物适应与进化重点实验室/青海省作物分子育种重点实验室, 青海西宁 810001;
2. 青海大学/畜牧兽医科学院, 青海西宁 810016;
3. 甘肃省治沙研究所/甘肃省沙生植物工程技术研究中心, 甘肃兰州 730070

作者简介:师生波(1963-),男,青海西宁人,博士,研究员,主要从事高山植物生理生态适应性研究,E-mail:sbshi@nwipb.cas.cn
基金资助:
青海省重大科技专项“耗牛提质增效技术集成与产业化示范”（2016-NK-A7）；国家自然科学基金项目（31660237）；甘肃省基础研究创新群体项目（1506RJIA155）；青海省自然科学基金面上项目（2016-ZJ-906）；青海省创新平台建设专项项目-青海省作物分子育种重点实验室（2017-ZJ-Y14）资助

Abstract

Abstract:

Plant response to a single environmental factor is often modified by another factor. Kobresia pygmaea was collected from the Alpine Grassland Ecosystem Research Station in the region of Three-River-Source. The chlorophyll fluorescence imaging of leaves was used to determine the interaction effects between soil drought and strong light besides measurement of photosynthetic pigments. The results showed that there was no difference in total chlorophyll contents (Chl a+b), and that chlorophyll a contents (Chl a) and the ratio of chlorophyll a to b (Chl a/b) decreased in soil drought treatment. The PSⅡ maximum and operating efficiency (F_v'/F_m' and F_q'/F_m') and the PSⅡ efficiency factor (F_q'/F_v') were significantly influenced by actinic light intensity, and the decrease of F_v'/F_m' and F_q'/F_m' were intensified by the treatment of soil drought. Non-photochemical quenching coefficient (NPQ) and relative limitation of PSⅡ photochemical efficiency (L_(PFD)) increased with the enhancement of actinic light intensity, and these trends were aggravated in soil drought treatment. GLM univariate analysis indicated that light intensity was the main factor, and excepting F_q'/F_m' and L_(PFD), the fluorescence parameters of NPQ and F_v'/F_m' exhibited interaction effect among actinic light and soil drought treatments. The results demonstrated that K. pygmaea could quickly respond to environmental light variations and had a strong tolerance to soil drought stress. The improvement of dissipation of non-photochemical energy was one of the important strategies for K. pygmaea to maintain effective performance of photosynthetic apparatus in alpine environments.

Key words: Chlorophyll fluorescence, Qinghai-Tibetan Plateau, Interaction effect, Kobresia pygmaea, Soil drought stress

摘要：

植物对单一环境因子的响应常会因另一因子存在而被修饰。本文主要采用叶绿素荧光图像分析技术，研究了土壤干旱对高山嵩草（Kobresia pygmaea）叶片光合功能的影响及与强光的交互效应。结果表明：土壤干旱胁迫可引起叶绿素a（Chl a）含量和Chl a/b比值的降低，对总叶绿素（Chl a+b）含量无影响。高光强可导致PSⅡ最大效率（F_v'/F_m'）、PSⅡ运行效率（F_q'/F_m'）和PSⅡ效率因子（F_q'/F_v'）的显著降低，且F_v'/F_m'和F_q'/F_m'的降低为土壤干旱所加剧；非光化学猝灭系数（NPQ）和光化学效率的相对限制（L_（PFD））随光强增大显著增加，也为干旱胁迫所加剧；PSⅡ反应中心开放比率（q_L）随光强的增大而降低，土壤干旱时降低趋势较小。方差分析表明，光强是影响光合机构光能利用和耗散的主要因素；PSⅡ非光化学能量耗散存在着两种胁迫因子的交互效应，但F_q'/F_m'和L_（PFD）则无。证实，高山嵩草能快速响应环境光强的变化，并对土壤干旱胁迫具有较强的耐受性，非光化学能量耗散的加强是光合功能有效维系的保护策略。

关键词: 叶绿素荧光, 青藏高原, 交互效应, 高山嵩草, 土壤干旱胁迫

CLC Number:

Q945.11

SHI Sheng-bo, LI Tian-cai, WANG Wei, DE Ke-jia, XU Xian-ying, WANG Qi, MA Jian-ping, LI Ai-de, KANG Cai-zhou. Interaction Effect of Soil Drought and Strong Light on Photosynthetic Performance of Kobresia Pygmaea in Qinghai-Tibetan Plateau[J]. Acta Agrestia Sinica, 2017, 25(4): 724-731,748.

师生波, 李天才, 王伟, 德科加, 徐先英, 王祺, 马剑平, 李爱德, 康才周. 土壤干旱和强光交互作用对青藏高原高山嵩草光合功能的影响[J]. 草地学报, 2017, 25(4): 724-731,748.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2017.04.006

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2017/V25/I4/724

References

[1] 周兴民,赵新全,曹广民,等. 中国嵩草草甸[M]. 北京:北京科学出版社,2001:62-68
[2] 李以康,林丽,张法伟,等. 小嵩草群落-高寒草甸地带性植被放牧压力下的偏途顶极群落[J]. 山地学报,2010,28(3):257-265
[3] 苗彦军,徐雅梅,呼天明,等. 高山嵩草种质资源评价研究[J].草业与畜牧,2008,8(11):10-13
[4] 王启基,李世雄,王文颖,等. 江河源区高山嵩草(Kobresia pygmaea)草甸植物和土壤碳、氮储量对覆被变化的响应[J]. 生态学报,2008,28(3):885-894
[5] 孙步功,龙瑞军,王长庭. 青藏高原冷龙岭南麓高寒小嵩草草甸植物种群物候学研究[J]. 草业科学,2007,24(8):16-20
[6] 王文颖,王启基,邓自发. 青海海北地区高山嵩草草甸植物群落的结构特征及其分布格局[J]. 植物生态学报,1998,22(4):336-343
[7] 王长庭,龙瑞军,丁路明. 青藏高原高寒嵩草草甸基本特征的研究[J]. 草业科学,2004,21(8):16-19
[8] 李希来,杨元武,张静,等. 不同退化程度"黑土滩"高山嵩草克隆生长特性[J]. 草业学报,2003,12(3):51-56
[9] 高新中,李希来,马桂花,等. 不同退化高寒草甸矮嵩草和高山嵩草无性系繁殖规律[J]. 草业与畜牧,2008,1:7-11
[10] 张守仁. 叶绿素荧光动力学参数的意义及讨论[J]. 植物学通报,1999,16:444-448
[11] Tikkanen M, Mekala N R, Aro E M. Photosystem Ⅱ photoinhibition-repair cycle protects Photosystem I from irreversible damage[J]. Biochimica et Biophysica Acta,2013,1837:210-215
[12] Yamori W, Hikosaka K, Way D A. Temperature response of photosynthesis in C₃,C₄,and CAM plants:temperature acclimation and temperature adaptation[J]. Photosynthesis Research,2014,119:101-117
[13] Lima Neto M C, Lobo A K M, Martins M O, et al. Dissipation of excess photosynthetic energy contributes to salinity tolerance:A comparative study of salt-tolerant Ricinus communis and salt-sensitive Jatropha curcas[J]. Journal of Plant Physiology,2014,171:23-30
[14] Baker N R. Chlorophyll fluorescence:A probe of photosynthesis in vivo[J]. Annual Review of Plant Biology,2008,59:89-113
[15] Baker N R,Rosenqvist E. Application of chlorophyll fluorescence and improve crop production strategies:an examination of future possibilities[J]. Journal of Experimental Botany,2004,55:1607-1621
[16] Oxborough K, Baker N R. Resolving chlorophyll a fluorescence images of photosynthetic efficiency into photochemical and non-photochemical components:Calculation of q_P and F_v'/F_m' without measuring F_o'[J]. Photosynthesis Research,1997,54:135-142
[17] Genty B, Briantais J M, Baker N R. The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence[J]. Biochimica et Biophysica Acta,1989,990:87-92
[18] Bilger W, Björkman O. Role of the xanthophyll cycle photoprotection elucidated by measurements of light-induced absorbance changes,fluorescence and photosynthesis in leaves of Hedera canariensis[J]. Photosynthesis Research,1990,25:173-185
[19] 许大全. 光合作用效率[M]. 上海:上海科学技术出版社,2002:163-170
[20] Shi S B, Shi R, Li M. Changes in photosynthesis of alpine plant Saussurea superba during leaf expansion[J]. Acta Physiol Plant,2015,37:235. DOI 10.1007/s11738-015-1987-4
[21] 朱广廉,钟诲文,张爱琴. 植物生理学实验[M]. 北京:北京大学出版社,1990:51-54
[22] 师生波,尚艳霞,朱鹏锦,等. 增补UV-B辐射对高山植物美丽风毛菊叶片PSⅡ光化学效率的影响[J]. 草地学报,2011,19(4):539-545
[23] Demmig-Adams B,Cohu CM,Muller O,et al. Modulation of photosynthetic energy conversion efficiency in nature:from seconds to seasons[J]. Photosynthesis Research,2012,113:75-88
[24] Osório M L, Osório J, Romano A. Photosynthesis,energy partitioning,and metabolic adjustments of the endangered Cistaceae species Tuberaria major under high temperature and drought[J]. Photosynthetica,2013,51:75-84
[25] Kramer D M, Johnson G, Kiirats O, et al. New fluorescence parameters for the determination of Q_A redox state and excitation energy fluxes[J]. Photosynthesis Research,2004,79:209-218
[26] Maxwell K, Johnson G N. Chlorophyll fluorescence-a practical guide[J]. Journal of Experimental Botany,2000,51:659-668
[27] Murchie E H, Niyogi K K. Manipulation of photoprotection to improve plant photosynthesis[J]. Plant Physiology,2011,155:86-92
[28] Niyogi K K, Truong T B. Evolution of flexible non-photochemical quenching mechanisms that regulate light harvesting in oxygenic photosynthesis[J]. Current Opinion in Plant Biology,2013,16:307-314
[29] 刘玉萍,周勇辉,吕婷,等. 青藏高原3种野生豌豆光合生理特性比较[J]. 草地学报,2017,25(1):122-129
[30] Murali N S, Teramura A H. Effectiveness of the solar UV-B radiation on the growth and physiology of field-grown soybean modified by water stress[J]. Photochemistry and Photobiology,1986,44:215-219
[31] Sullivan J H, Teramura A H. Field study of the interaction between solar ultraviolet-B radiation and drought on photosynthesis and growth in soybean[J]. Plant Physiology,1990,92:141-146
[32] 郑洁,郭延平,胡美君. 光温交互作用对柑橘植株叶绿素荧光和D1蛋白的影响[J]. 浙江大学学报(农业与生命科学版),2008,34(6):629-634
[33] 李金鹏,罗丹娜,马彦,等. 光照和氮交互作用对3种彩叶玉簪光合作用及荧光特性的影响[J]. 东北林业大学学报,2012,40(7):56-59,63
[34] 张翠萍,孟平,李建中,等. 磷元素和土壤酸化交互作用对核桃幼苗光合特性的影响[J]. 植物生态学报,2014,38(12):1345-1355
[35] 姜罡丞,贾晓红. 不同海拔高度上的嵩草属三个种营养器官显微结构的比较[J]. 河南大学学报(自然科学版),1999,29:63-68
[36] Ingrisch J, Biermann T, Seeber E, et al. Carbon pools and fluxes in a Tibetan alpine Kobresia pygmaea pasture partitioned by coupled eddy-covariance measurements and ¹³CO₂ pulse labeling[J]. Science of the Total Environment,2015,505:1213-1224
[37] Dorji T, Totland R, Moe S, et al. Plant functional traits mediate reproductive phenology and success in response to experimental warming and snow addition in Tibet[J]. Global Change Biology,2013,19:459-472

Interaction Effect of Soil Drought and Strong Light on Photosynthetic Performance of Kobresia Pygmaea in Qinghai-Tibetan Plateau

土壤干旱和强光交互作用对青藏高原高山嵩草光合功能的影响

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHANG Zhong-hua, MA Li, ZHOU Bing-rong, SONG Ming-hua, XU Wen-hua, DENG Yan-fang, WANG Fang, SHE Yan-di, ZHANG Qian, YAO Bu-qing, MA Zhen, ZHOU Hua-kun. Esponse of the Functional Diversity of Dominant Plants to Warming and Simulated Grazing in Alpine Meadow [J]. Acta Agrestia Sinica, 2021, 29(S1): 225-232.
[2]	WANG Tan-guo-yan, MA Zhi-yuan, LI Pei-yang, GUO Jun-hong, ZHANG Jia-yi, JIANG Sheng-jing. Effects of Short-Term Warming on Arbuscular Mycorrhizal Fungi in the Rhizosphere of Different Plant Species in Alpine Meadows on the Qinghai-Tibetan Plateau [J]. Acta Agrestia Sinica, 2021, 29(9): 1959-1966.
[3]	SUN Hua-fang, LI Xi-lai, LI Cheng-yi, ZHANG Jing, LIN Chun-ying, JIN Li-qun, YANG Xin-guang. Changes of CO₂ Fluxes and Chlorophyll Fluorescence Parameters of Biological Soil Crusts in a 19-year Artificial Grassland in the Source Zone of the Yellow River [J]. Acta Agrestia Sinica, 2021, 29(5): 894-903.
[4]	QIN Jin-ping, LIU Ying, MA Yu-shou, WANG Yan-long, LI Shi-xiong. Effects of Rest Grazing on Vegetation Characteristics and Photosynthetic Physiology of Dominant Species in Degraded Alpine Grasslands During Greenup Period [J]. Acta Agrestia Sinica, 2021, 29(5): 1034-1042.
[5]	WANG Jian-qiu, CAO Zi-lin, WANG Xiao-li, ZHANG Jin-long, PENG Chen-yin. Effects of Lead Stress on Growth,Photosynthetic Physiology and Chlorophyll Fluorescence of Silene viscidula [J]. Acta Agrestia Sinica, 2021, 29(11): 2422-2427.
[6]	JIANG Cui-xia, HU Chang-sheng, WEI Hai-yan, WANG Xian-ju, DING Lu-ming. The Effect of Modulating Different Utilizing Intensity in Summer Season on Forage Quality and Soil Nutrients of Winter & Spring Pasture on Qinghai-Tibetan Plateau [J]. Acta Agrestia Sinica, 2020, 28(5): 1473-1477.
[7]	WANG Jun, ZHAO Gui-qin, CHAI Ji-kuan, WANG Miao-miao, JIA Xue-jie, Guo Zhi-fei, SUN Lei-lei, Nie Xiu-mei. Effect of Barley Yellow Dwarf Virus Infection on Photosynthesis and Chlorophyll Fluorescence Parameters of Oat [J]. Acta Agrestia Sinica, 2020, 28(4): 923-931.
[8]	ZHANG Xiao-fang, ZHANG Chun-ping, DONG Quan-min, YANG Xiao-xia, LIU Wen-ting, YU Yang, YANG Zeng-zeng, WEI Lin-na, ZHANG Yan-fen, CHU Hui, Li Cai-di, FENG Bin. Study on Plant Community Structure Characteristics of Alpine Mixed-seeding Grassland in Three Rivers Source Regions [J]. Acta Agrestia Sinica, 2020, 28(4): 1090-1099.
[9]	LIU Cheng-gong, SUN Gao-jie, DUAN Na, LI Qing-he, ZHU Tian-tian, LI Hui-qing. Effects of Nitrogen and Phosphorus Addition on Chlorophyll Content and Photosynthetic Activity of Nitraria tangutorum [J]. Acta Agrestia Sinica, 2020, 28(3): 694-702.
[10]	YANG Jing, ZHANG Qian, YAO Bao-hui, CAI Zhi-yuan, WANG Chang, GUO Huai-liang, SU Jun-hu. Effects of Plateau Zokor Disturbance on Soil Physicochemical Properties and Plant Biomass in Alpine Meadow [J]. Acta Agrestia Sinica, 2020, 28(2): 492-499.
[11]	LIANG Huan, WEI Bao, CHEN Jing, GONG Wen-long, MA Lin, WANG Xue-min, WANG Zan. Evaluation of the Drought Resistance in Alfalfa Germplasm by Chlorophyll Fluorescence Parameters at Seedling Stage [J]. Acta Agrestia Sinica, 2020, 28(1): 45-55.
[12]	LI Jun, QI Guang-ping, KANG Yan-xia, MA Yan-lin, YIN Min-hua, LI Xiao-min, LI Zhi. Photosynthetic Characteristics,Chlorophyll Fluorescence Parameters and Biomass of Alfalfa under Different Irrigation Treatments [J]. Acta Agrestia Sinica, 2019, 27(6): 1569-1576.
[13]	XU Zhe, REN Jian, TIAN Ying, MI Nan. Effects of Exogenous ABA on Photosynthetic Characteristics of Lolium perenne under Drought Stress [J]. Acta Agrestia Sinica, 2019, 27(5): 1243-1249.
[14]	YAO Xi-xi, GONG Xu-yin, ZHANG Li-ping, JIAO Ting, TAO Hai-xia, GUO Bin, ZHANG Ai-qing, WU Jian-ping. Effects of Grazing and Long-term Fencing on Nutritive Values of Dominant Species in Alpine Meadow of Qilian Mountains [J]. Acta Agrestia Sinica, 2018, 26(6): 1354-1362.
[15]	QIAO Yong-gang, WANG Yong-fei, CHEN Liang, CUI Fen-fen, CAO Ya-ping, SONG Yun. Effects of Low Temperature above Zero on Ultrastructure and Physiological Characteristicsof Taraxacum officinale [J]. Acta Agrestia Sinica, 2018, 26(6): 1467-1472.