Study on the Relevance between Genetic Diversity of Gymnocarpos przewalskii Populations and Geographic Climatic Factors

doi:10.11733/j.issn.1007-0435.2018.01.008

Acta Agrestia Sinica ›› 2018, Vol. 26 ›› Issue (1): 70-76.DOI: 10.11733/j.issn.1007-0435.2018.01.008

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Study on the Relevance between Genetic Diversity of Gymnocarpos przewalskii Populations and Geographic Climatic Factors

XU Zhen-peng¹, WAN Tao¹, CAI Ping², ZHANG Xiao-ming¹, YI Wei-dong³, WAN Yi-chao¹

1. College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010019, China;
2. Xilingol Vocational College, Xilinhot, Inner Mongolia 026000, China;
3. College of Agricultural, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010019, China

Received:2017-07-07 Revised:2018-03-15 Online:2018-02-15 Published:2018-05-25

裸果木种群遗传多样性特点及与地理气候因子关联研究

徐振朋¹, 宛涛¹, 蔡萍², 张晓明¹, 伊卫东³, 宛诣超¹

1. 内蒙古农业大学草原与资源环境学院, 内蒙古呼和浩特 010019;
2. 锡林郭勒职业学院, 内蒙古锡林浩特 026000;
3. 内蒙古农业大学农学院, 内蒙古呼和浩特 010019

通讯作者: 宛涛
作者简介:徐振朋(1988-),男,内蒙古赤峰人,博士,主要从事牧草种质资源研究,E-mail:947464263@qq.com
基金资助:
内蒙古自然科学基金项目（2016MS0324）；内蒙古自治区研究生科研创新项目（B20151012919）资助

Abstract

Abstract:

To study 12 species of Gymnocarpos przewalskii in Northwest desert area，15 pairs of primers selected by ISSR marker were used to analyze the correlation of genetic diversity and geographic and climatic factors. The results showed that the polymorphic loci percentage (PPL) was 88.81%, the population level was 69.93%; and the variation range of Nei's gene diversity index (He) was 0.2356~0.2697. The variation range of Shannon's Information index (I) was 0.3509~0.4057, which indicated high genetic diversity. The coefficient of gene differentiation (Gst) was 0.2790, with a small genetic differentiation between each group and a rich genetic variation in group. Mantel test showed that the population genetic distance was related to geographical distance(R²=0.2144). Using the ntsyspc-2.1 software to do the unweighted averages (UPGMA) cluster analysis, the genetic similarity coefficient for each group ranged from 0.81 to 0.99. According to the genetic distance, 12 Gymnocarpos przewalskii species divided into four groups. According to the correlation analysis, the latitude and altitude had a positive effect and average annual temperature had negative influence on the population genetic diversity.

Key words: Gymnocarpos przewalskii, Genetic diversity, Genetic structure, Environmental factors

摘要：

本文利用ISSR标记技术，选取15对引物，对分布于西北荒漠区12个裸果木种群的遗传多样性以及与地理气候因子的相关性进行了分析。结果表明：群体内多态性位点百分率（PPL）为88.81%，种群水平为69.93%；Nei’s基因多样性指数（He）变化范围为0.2356~0.2697，Shannom信息指数（I）变化范围为0.3509~0.4057，具较高的遗传多样性水平。基因分化系数（Gst）为0.2790，种群间遗传分化较小，种群内存有丰富的遗传变异。经Mantel检验，种群遗传距离与地理距离有相关性（R²=0.2144）。利用NTSYSpc-2.1软件进行UPGMA法聚类，各种群样品间的遗传相似系数在0.81～0.99之间，按照遗传距离远近，裸果木12个种群可分为4大类群。经相关性分析，种群遗传多样性与纬度、海拔存在正相关（P<0.05）与年均温存在负相关（P<0.05）。

关键词: 裸果木, 遗传多样性, 遗传结构, 环境因子

CLC Number:

XU Zhen-peng, WAN Tao, CAI Ping, ZHANG Xiao-ming, YI Wei-dong, WAN Yi-chao. Study on the Relevance between Genetic Diversity of Gymnocarpos przewalskii Populations and Geographic Climatic Factors[J]. Acta Agrestia Sinica, 2018, 26(1): 70-76.

徐振朋, 宛涛, 蔡萍, 张晓明, 伊卫东, 宛诣超. 裸果木种群遗传多样性特点及与地理气候因子关联研究[J]. 草地学报, 2018, 26(1): 70-76.

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Study on the Relevance between Genetic Diversity of Gymnocarpos przewalskii Populations and Geographic Climatic Factors

裸果木种群遗传多样性特点及与地理气候因子关联研究

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