Development of the Wettable Powder of Fungal Endophyte HL-1 and Evaluation of Its Herbicidal Activity

doi:10.11733/j.issn.1007-0435.2019.05.024

Acta Agrestia Sinica ›› 2019, Vol. 27 ›› Issue (5): 1301-1308.DOI: 10.11733/j.issn.1007-0435.2019.05.024

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Development of the Wettable Powder of Fungal Endophyte HL-1 and Evaluation of Its Herbicidal Activity

ZHU Hai-xia^1,2,3,4, MA Yong-qiang^1,2,3,4

1. Academy of Agriculture and Forestry Sciences of Qinghai University, Xining, Qinghai Province 810016;
2. State Key Laboratory of Plateau Ecology and Agriculture of Qinghai University, Xining, Qinghai Province 810016;
3. Scientific Observing and Experimental Station of Crop Pest in Xining in Ministry of Agriculture, Xining, Qinghai Province 810016;
4. Key Laboratory of Agricultural Integrated Pest Management in Qinghai Province, Xining, Qinghai Province 810016, China

Received:2019-04-25 Revised:2019-09-04 Online:2019-10-15 Published:2019-11-09

内生菌HL-1可湿性粉剂研制及其除草活性评价

朱海霞^1,2,3,4, 马永强^1,2,3,4

1. 青海大学农林科学院, 青海西宁 810016;
2. 青海大学省部共建三江源生态与高原农牧业国家重点实验室, 青海西宁 810016;
3. 农业部西宁作物有害生物科学观测实验站, 青海西宁 810016;
4. 青海省农业有害生物综合治理重点实验室, 青海西宁 810016

通讯作者: 马永强
作者简介:朱海霞(1985-),女,青海西宁人,副研究员,主要从事杂草生物防治研究,E-mail:zhuhaixia0101@163.com
基金资助:
国家自然科学基金项目（31560518）；中国科学院“西部之光”人才培养引进计划项目（201523）共同资助

Abstract

Abstract: This study is aimed to develop wettable powder of endophyte HL-1 with high herbicidal activity. The optimum carbon,nitrogen source,solid fermentation material and suitable carriers and adjuvant for preparation of wettable powder were screened by single factor method. The herbicidal effect of this strain in pots in vivo and in fields were tested. The optimum carbon and nitrogen sources were saccharose and yeast powder respectively,the optimum solid fermentation material of the strain was wheat bran. The optimized carriers and adjuvant of HL-1 were diatomaceous earth,bentonite,carboxymethyl cellulose and amylogen. In the pot and filed plot experiment,wettable powder of HL-1 showed high virulence to Stellaria media L.,Chenopodium album L,Chenopodium glaucum L. and Elsholtzia densa Benth. In pot experiment,the disease incidence and fresh weight effect to Stellaria media L. were 85.50% and 84.74% respectively. In filed plot experiment,the disease incidence and fresh weight effect to Stellaria media L.were 84.50% and 85.36% respectively. The strain has the potential to be further developed as a biological herbicide,which is expected to replace or partially replace chemical herbicides and be applied to weed control in farmland.

Key words: Fungal endophyte HL-1, Wettable powder, Weeds, Control effect to weeds

摘要： 为研究内生菌HL-1可湿性粉剂的制备材料及其除草活性，本试验采用单因素法筛选生防除草内生菌HL-1最适碳氮源、固体发酵基质、适宜载体和助剂，制备可湿性粉剂，并进行盆栽和田间小区接种，测定其除草活性。结果表明：HL-1最适碳氮源分别是蔗糖和酵母浸粉，最适固体发酵基质为麦麸，最适载体与助剂为硅藻土、膨润土、羧甲基纤维素和可溶性淀粉；在苗期盆栽和田间小区试验中，菌株HL-1可湿性粉剂对繁缕（Stellaria media L.）、灰藜（Chenopodium album L.）、藜（Chenopodium glaucum L.）和密花香薷（Elsholtzia densa Benth.）4种杂草均表现出较强致病性，盆栽试验中对繁缕的发病率和鲜重效分别是85.50%和84.74%，小区试验中对繁缕的发病率和鲜重效分别是84.50%和85.36%。本试验制备的内生菌HL-1可湿性粉剂，可作为生防菌剂有效控制繁缕、灰藜等杂草。

关键词: 内生菌HL-1, 可湿性粉剂, 农田杂草, 除草活性

CLC Number:

S431.14

ZHU Hai-xia, MA Yong-qiang. Development of the Wettable Powder of Fungal Endophyte HL-1 and Evaluation of Its Herbicidal Activity[J]. Acta Agrestia Sinica, 2019, 27(5): 1301-1308.

朱海霞, 马永强. 内生菌HL-1可湿性粉剂研制及其除草活性评价[J]. 草地学报, 2019, 27(5): 1301-1308.

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Development of the Wettable Powder of Fungal Endophyte HL-1 and Evaluation of Its Herbicidal Activity

内生菌HL-1可湿性粉剂研制及其除草活性评价

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