Screening and Identification of Growth-promoting Bacteria from Three Rare Plants Rhizosphere Soil in Desert and Its Effect on the Growth of Sorghum Seedlings

doi:10.11733/j.issn.1007-0435.2020.04.031

Acta Agrestia Sinica ›› 2020, Vol. 28 ›› Issue (4): 1121-1128.DOI: 10.11733/j.issn.1007-0435.2020.04.031

Previous Articles Next Articles

Screening and Identification of Growth-promoting Bacteria from Three Rare Plants Rhizosphere Soil in Desert and Its Effect on the Growth of Sorghum Seedlings

GUO Yu-qing¹, ZHAO Shi-chao², XU Dao-long², GUO Yang-nan¹, HE An-min¹, CHANG Jian-hong¹, BAO Yu-ying²

1. The Technology Research Institute of Shendong Coal Company in China Shenhua Group, Ordos, Inner Mongolia 017200, China;
2. Key Laboratory of Forage and Endemic Crop Biotechnology, Ministry of Education, State Key Laboratory of Reproductive Regulatory and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot, Inner Mongolia 010010, China

Received:2020-01-14 Revised:2020-03-29 Online:2020-08-15 Published:2020-07-28

3种荒漠珍稀植物根际促生菌的筛选、鉴定及对高粱幼苗生长的影响

郭雨晴¹, 赵世超², 徐道龙², 郭洋楠¹, 贺安民¹, 常建鸿¹, 包玉英²

1. 中国神华能源股份有限公司神东煤炭分公司技术研究院, 内蒙古鄂尔多斯 017200;
2. 内蒙古大学生命科学学院牧草与特色作物生物技术教育部重点实验室, 省部共建草原家畜生殖调控与繁殖国家重点实验室, 内蒙古呼和浩特 010071

通讯作者: 包玉英
作者简介:郭雨晴(1996-),女,内蒙古巴彦淖尔人,硕士研究生,主要从事菌根生物学研究,E-mail:15904898045@163.com
基金资助:
神东煤炭分公司科技创新项目（201691548939）；内蒙古自然科学基金项目（2015ZD04）；内蒙古自治区科技厅重大项目（ZDZX2018065）；国家自然科学基金（30960309）资助

Abstract

Abstract: In order to obtain excellent plant growth promoting rhizobacteria,growth-promoting strains were isolated and screened from rare plants rhizosphere soil in desert. Strains were identified by 16S rDNA gene sequence and physiological and biochemical characteristics,and analyzed their growth-promoting effect by germination and pot experiments. The results showed that the concentrations of total nitrogen,available potassium and organic matter were extremely deficient in rhizosphere soil,and the soil were weakly alkaline. We isolated 5 strains (BN5,HN7,BP4,HD3 and SD5) which had the ability to fix nitrogen,solubilize phosphorus and produce proteinase. They were belong to Sphingomonas sp.,Agrobacterium tumefaciens,Arthrobacter sp.,Fictibacillus phosphorivorans and Bacillus simplex,respectively. They all showed growth-promoting ability on Sorghum seedlings,and BP4 was the best. The germination potential,germination rate,bud length,root length,plant height and fresh weight of Sorghum seedlings treated by BP4 increased by 55.30%,23.16%,54.52%,40.24%,22.59%,and 26.67%,respectively,when compared with the control group. These results provide a theoretical basis for research on the construction of an excellent desert microbiological resource bank in the future.

Key words: Rare plants in the desert, Growth-promoting rhizobacteria, Nitrogen-fixing, Phosphate-solubilizing, Proteinase-producing

摘要： 为获得优良植物根际促生菌，本研究从荒漠珍稀植物根际土中分离筛选促生菌，结合16S rDNA基因序列、生理生化特征对其进行鉴定，并通过发芽和盆栽试验分析其促生效果。结果显示：土壤全氮、有效钾、有机质含量较少，呈弱碱性；分离得到5株可固氮、溶磷、产蛋白酶的菌株BN5，HN7，BP4，HD3及SD5，其分别隶属于鞘氨醇单胞菌属（Sphingomonas sp.）、根癌农杆菌属（Agrobacterium tumefaciens）、节杆菌属（Arthrobacter sp.）、磷酸镰孢菌属（Fictibacillus phosphorivorans）及单纯芽孢杆菌属（Bacillus simplex）；5株菌对高粱（Sorghum bicolor）幼苗均表现出促生作用，效果最明显的为菌株BP4，其处理的高粱幼苗发芽势、发芽率、芽长、根长、株高及鲜重较对照组分别增加了55.30%，23.16%，54.52%，40.24%，22.59%，26.67%。本研究结果可为今后荒漠优良菌种资源库的建设提供理论依据。

关键词: 荒漠珍稀植物, 根际促生菌, 固氮, 溶磷, 产蛋白酶

CLC Number:

GUO Yu-qing, ZHAO Shi-chao, XU Dao-long, GUO Yang-nan, HE An-min, CHANG Jian-hong, BAO Yu-ying. Screening and Identification of Growth-promoting Bacteria from Three Rare Plants Rhizosphere Soil in Desert and Its Effect on the Growth of Sorghum Seedlings[J]. Acta Agrestia Sinica, 2020, 28(4): 1121-1128.

郭雨晴, 赵世超, 徐道龙, 郭洋楠, 贺安民, 常建鸿, 包玉英. 3种荒漠珍稀植物根际促生菌的筛选、鉴定及对高粱幼苗生长的影响[J]. 草地学报, 2020, 28(4): 1121-1128.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2020.04.031

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2020/V28/I4/1121

References

[1] 荣良燕,柴强,姚拓,等. 复合微生物接种剂替代部分化肥对豌豆间作玉米的促生效应[J]. 草业学报,2015,24(2):22-30
[2] Kamaei R,Faramarzi F,Parsa M,et al. The effects of biological,chemical,and organic fertilizers application on root growth features and grain yield of Sorghum[J]. Journal of Plant Nutrition,2019,42(18):2221-2233
[3] Fan X H,Zhang S A,Mo X D,et al. Effects of plant growth-promoting rhizobacteria and N source on plant growth and N and P uptake by tomato grown on calcareous soils[J]. Pedosphere,2017,27(6):1027-1036
[4] Keswani C,Prakash O,Bharti N,et al. Re-addressing the biosafety issues of plant growth promoting rhizobacteria[J]. The Science of the Total Environment,2019,690:841-852
[5] Hashem A,Tabassum B,Abd-Allah E F. Bacillus subtilis:a plant-growth promoting rhizobacterium that also impacts biotic stress[J]. Saudi Journal of Biological Sciences,2019,26(6):1291-1297
[6] 刘丹丹,李敏,刘润进. 我国植物根围促生细菌研究进展[J]. 生态学杂志,2016,35(3):815-824
[7] 刘少芳,王若愚. 植物根际促生细菌提高植物耐盐性研究进展[J]. 中国沙漠,2019,39(2):1-12
[8] Sarawaneeyaruk S,Lorliam W,Krajangsang S,et al. Enhancing plant growth under municipal wastewater irrigation by plant growth promoting rhizospheric Bacillus spp[J]. Journal of King Saud University Science,2019,31(3):384-389
[9] Goswami D,Dhandhukia P,Patel P,et al. Screening of PGPR from saline desert of Kutch:growth promotion in Arachis hypogea by Bacillus licheniformis A2[J]. Microbiological Research,2014,169(1):66-75
[10] 刘国强,旭格拉·哈布丁,艾山江,等. 黑果枸杞根际促生菌的筛选鉴定及促生能力分析[J]. 厦门大学学报(自然科学版),2019,58(1):56-62
[11] Mahanty T,Bhattacharjee S,Goswami M,et al. Biofertilizers:a potential approach for sustainable agriculture development[J]. Environmental Science and Pollution Research,2017,24(4):3315-3335
[12] 党晓宏,高永,蒙仲举,等. 西鄂尔多斯荒漠灌丛生态系统碳密度[J]. 中国沙漠,2018,38(2):352-362
[13] 智颖飙,李红丽,崔艳,等. 孑遗植物四合木(Tetraena mongolica)迁地保护中的光合作用日变化特征与生理生态适应性[J]. 生态环境学报,2015,24(1):14-21
[14] 孙安安,智颖飙,姜平平,等. 西鄂尔多斯4种荒漠植物光合作用特征与差异性[J]. 生态学报,2019,39(13):4944-4952
[15] 段义忠,杜忠毓,王海涛. 西北干旱区孑遗濒危植物四合木(Tetraena mongolica)叶绿体基因组特征研究及比较分析[J]. 植物研究,2019,39(5):653-663
[16] 刘亚琪,王文颖,崔彦农,等. 霸王环核苷酸门控通道基因ZxCNGC5的克隆及表达模式分析[J]. 植物生理学报,2018,54(1):157-164
[17] 石松利,王迎春,周红兵,等. 濒危种四合木与其近缘种霸王水分关系参数和光合特性的比较[J]. 生态学报,2012,32(4):159-169
[18] 孙艳霞,杨九艳,乔宜青,等. 阿拉善高原红砂(Reaumuria songarica)种群空间分布格局[J]. 中国沙漠,2020,40(1):105-115
[19] 谭锦,杨建英,侯健,等. 乌海市几种蒺藜科灌木与土壤养分空间格局关系[J]. 中国水土保持科学,2019,17(4):93-103
[20] 史国英,曾泉,农泽梅,等. 一株高效甘蔗内生固氮菌NN08200的鉴定及其对甘蔗的促生长作用[J]. 微生物学通报,2019,46(6),1336-1345
[21] 马骢毓,张英,马文彬,等. 黄芪根际促生菌(PGPR)筛选与特性研究[J]. 草业学报,2017,26(1):149-159
[22] 陈鸿图. 高产中性蛋白酶菌株选育及产酶特性研究[D]. 广州:华南理工大学,2012:11
[23] 沈萍,陈向东. 微生物学实验[M]. 北京:高等教育出版社,2007:16-17
[24] 吴才武,夏建新,段峥嵘. 土壤有机质测定方法述评与展望[J]. 土壤,2015,47(3):453-460
[25] 赵兰坡,姜岩. 土壤磷酸酶活性测定方法的探讨[J]. 土壤通报,1986,17(3):138-141
[26] 魏立娟,王玉琴,杨成德,等. 青海省海晏县线叶嵩草内生细菌的生物功能鉴定及测定[J]. 草地学报,2018,26(3):764-769
[27] Kumar A,Singh R,Yadav A,et al. Isolation and characterization of bacterial endophytes of Curcuma longa L.[J]. 3 Biotech,2016,6(1):60
[28] 邓振山,李买平,郝雷,等. 巨菌草内生细菌多样性及其促生特性[J]. 草地学报,2019,27(5):1213-1221
[29] 刘婷. 高寒草甸优势植物根际促生菌资源评价及菌种鉴定[D]. 兰州:甘肃农业大学,2016:23-24
[30] Li Y,Wu C Y,Zhou M Y,et al. Diversity of cultivable protease-producing bacteria in Laizhou bay sediments,Bohai sea,China[J]. Frontiers in Microbiology,2017,8:405
[31] Rehbar S,Batool R. Protease production by Brevundimonas sp. and Trabulsiella guamensis isolated from contaminated soil[J]. Rendiconti Lincei,2017,28(3):529-534
[32] 东秀珠,蔡妙英. 常见细菌系统鉴定手册[M]. 北京:科学出版社,2001:169
[33] 王天乐,卫智军,刘文亭,等. 不同放牧强度下荒漠草原土壤养分和植被特征变化研究[J]. 草地学报,2017,25(4):711-716
[34] Liang T Y,Yang G,Ma Y X,et al. Seasonal dynamics of microbial diversity in the rhizosphere of Ulmus pumila L. var. sabulosa in a steppe desert area of Northern China[J]. PeerJ,2019,7:e7526
[35] 王媚臻,毕浩杰,金锁,等. 林分密度对云顶山柏木人工林林下物种多样性和土壤理化性质的影响[J]. 生态学报,2019,39(3):981-988
[36] 何凌仙子,贾志清,刘涛,等. 植物适应逆境胁迫研究进展[J]. 世界林业研究,2018,31(2):13-18
[37] Hansen V,Bonnichsen L,Nunes I,et al. Seed inoculation with Penicillium bilaiae and Bacillus simplex affects the nutrient status of winter wheat[J]. Biology and Fertility of Soils:Cooperating Journal of International Society of Soil Science,2020,56(1):97-109
[38] Ali A,Mohanta T K,Asaf S,et al. Biotransformation of benzoin by Sphingomonas sp. LK11 and ameliorative effects on growth of Cucumis sativus[J]. Archives of Microbiology,2019,201(5):591-601
[39] Liu J,Lin H,Dong Y B,et al. Elucidating the biodegradation mechanism of tributyl phosphate (TBP) by Sphingomonas sp. isolated from TBP-contaminated mine tailings[J]. Environmental Pollution,2019,250:284-291
[40] Wang X D,Liu X J,Wang H L,et al. Utilization and degradation of imazaquin by a naturally occurring isolate of Arthrobacter crystallopoietes[J]. Chemosphere,2007,67(11):2156-2162
[41] Xiao X,Li Y,Qin J L,et al. An optimized Agrobacterium tumefaciens-mediated transformation system for random insertional mutagenesis in Fonsecaea monophora[J]. Journal of Microbiological Methods,2020,170:105838
[42] Zheng Z Q,Zheng J S,Liu H L,et al. Complete genome sequence of Fictibacillus phosphorivorans G25-29,a strain toxic to nematodes[J]. Journal of Biotechnology,2016,239:20-22
[43] 张雅柔,安慧,刘秉儒,等. 短期氮磷添加对荒漠草原土壤活性有机碳的影响[J]. 草业学报,2019,28(10):12-24
[44] Khan N,Zandi P,Ali S,et al. Corrigendum:impact of salicylic acid and PGPR on the drought tolerance and phytoremediation potential of Helianthus annus[J]. Frontiers in Microbiology,2019,10:2222
[45] Chiappero J,del Rosario Cappellari L,Alderete L G S,et al. Plant growth promoting rhizobacteria improve the antioxidant status in Mentha piperita grown under drought stress leading to an enhancement of plant growth and total phenolic content[J]. Industrial Crops & Products,2019,139:111553
[46] 马骢毓,姚拓. 黑果枸杞根际促生菌筛选与特性研究[J]. 草原与草坪,2018,38(2):73-79
[47] 冯利芳,李素琴,刘鹏烩,等. 清香大曲中性蛋白酶产生菌的分离鉴定及应用研究[J]. 食品科技,2018,43(11):1-6
[48] Tahir M,Ahmad I,Shahid M,et al. Regulation of antioxidant production,ion uptake and productivity in potato (Solanum tuberosum L.) plant inoculated with growth promoting salt tolerant Bacillus strains[J]. Ecotoxicology and Environmental Safety,2019,178:33-42
[49] Coṣkun N,Plrlak L. Effect of plant growth promoting rhizobacteria (PGPR) on branching and growing of apple sapling[J]. Erwerbs-Obstbau,2017,59(4):309-313
[50] Sivagnanam S,Logeswari P,Valentine A,et al. Role of Curtobacterium herbarum strain CAH5 on aluminum bioaccumulation and enhancement of Lactuca sativa growth under aluminum and drought stresses[J]. Ecotoxicology and Environmental Safety,2019,183:109573
[51] Liu K,Newman M,McInroy J A,et al. Selection and assessment of plant growth-promoting rhizobacteria for biological control of e00073-17 multiple plant diseases[J]. Phytopathology,2017,107(8):928-936

Screening and Identification of Growth-promoting Bacteria from Three Rare Plants Rhizosphere Soil in Desert and Its Effect on the Growth of Sorghum Seedlings

3种荒漠珍稀植物根际促生菌的筛选、鉴定及对高粱幼苗生长的影响

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 13

Recommended Articles

Metrics

Comments

[1]	ZHAO Shu-dong, LI Jian-hong. The Study on Screening and Characteristics of Rhizosphere Growth-promoting Bacteria of Poa alpigena L. [J]. Acta Agrestia Sinica, 2021, 29(9): 1885-1891.
[2]	ZHANG Xin-fei, SHE Mu-zi, LI Han-yu, JING Song, JIANG Hui-na, GAO Hao, ZHU Yan-xiao, FU Juan-juan. Growth Promotion Mechanisms of flavobacterium succinicans and Their Physiological Regulation on the Growth and Stress Tolerance in Lolium perenne [J]. Acta Agrestia Sinica, 2021, 29(8): 1704-1711.
[3]	ZHANG Wan-tong, LI Chao-qun, YU Lu, SHAO Xin-qing. Study on the Effect of the Plant Growth-Promoting Rhizobacteria Bio-fertilizer Instead of Chemical Fertilizer in Alpine Meadow [J]. Acta Agrestia Sinica, 2021, 29(7): 1423-1429.
[4]	YANG Wan-qiu, JING Jie, ZHU Ling, GAO Yong-heng. Screening and Characteristics of Plant Growth-Promoting Rhizobacteria From Alpine Meadow Plants in Northwestern Sichuan [J]. Acta Agrestia Sinica, 2021, 29(6): 1174-1182.
[5]	ZHAO Hui-hui, FANG Zhi-gang, MA Rui, LOU Lai-qing, CAI Qing-sheng. Screening of Cadmium-Tolerant and Plant Growth-Promoting Rhizobacteria and Their Effects on Cadmium Uptake and Accumulation by Lolium multiflorum Lam. [J]. Acta Agrestia Sinica, 2017, 25(3): 554-560.
[6]	DONG Rong-shu, ZHANG Jie, HUANG Yan-xia, Bai Chang-ju, HUAN Heng-fu, WANG Wen-qiang, LIU Guo-dao. Effects of Different Rhizobium Strains on Stylosanthes guianensis ‘Reyan No.5’ [J]. Acta Agrestia Sinica, 2014, 22(3): 605-610.
[7]	HAN Hua-wen, SUN Li-na, YAO Tuo, ZHANG Ying, WANG Guo-ji. Effects of Effective Microbial Inoculants on Alfalfa Growth Character [J]. , 2013, 21(2): 353-359.
[8]	HAN Yu-zhu, ZHAO Jian-jun, ZENG Bing, MING Xiao-yan, ZHU Quan-wei. Isolation and the Phosphate Solubilizing Capacity of Italian Ryegrass Rhizosphere Phosphate-Solubilizing Microorganism [J]. , 2011, 19(5): 766-770.
[9]	ZHANG Yu-long, LU Xiao-liang, YANG Chen-de. A Preliminary Study on the Isolation and Characterization of Inorganic Phosphate-solubilizing Bacteria in Soil of Alpine Grasslands in Eastern Qilian Mountains [J]. , 2011, 19(4): 560-564.
[10]	ZHANG Kun, YAO Tuo, ZHANG De-gang, XIN Guo-rong . Effect of Associative Nitrogen-fixing Biofertilizer Produced with Different Carriers on Growth and Nitrogen Fixing Ability of Hordeum vulgare var. nudum Hook.f. [J]. , 2010, 18(3): 426-430.
[11]	LI Yu-e, YAO Tuo, RONG Liang-yan. Characteristics of IAA Secretion and Phosphate Dissolving of Phosphate-solubilizing Bacteria and Its Effect on Alfalfa Growth [J]. , 2010, 18(1): 84-88.
[12]	ZHU Ying, YAO Tuo, LI Yu-e, SUN Hong-yang. Screening of Phosphate-solubilizing Bacteria and Their Acting Mechanisms in the Rhizosphere of Red Clover [J]. , 2009, 17(2): 259-263.
[13]	Guan Xiuqing, Du Qianyou, Yu Jingzhao. Isolation and Characteristics of N₂-fixers From Rhizosphere of Plant Leymus chinensis in the Rangeland of Inner Mongolia [J]. , 1997, 5(2): 101-107.