Antagonism of Endophytic Bacteria ZJ1 from Buddleja lindleyana Fortune and Identification of Antifungal Lipopetide Metabolites

doi:10.11733/j.issn.1007-0435.2021.03.003

Acta Agrestia Sinica ›› 2021, Vol. 29 ›› Issue (3): 434-442.DOI: 10.11733/j.issn.1007-0435.2021.03.003

Previous Articles Next Articles

Antagonism of Endophytic Bacteria ZJ1 from Buddleja lindleyana Fortune and Identification of Antifungal Lipopetide Metabolites

REN Lu, LIU Xiao-feng, ZHOU Jian-bo, YIN Hui, ZHANG Bao-jun, ZHAO Xiao-jun

College of Plant Protection, Shanxi Agricultural University, Taiyuan, Shanxi Province 030031, China

Received:2020-10-04 Revised:2020-11-06 Online:2021-03-15 Published:2021-04-02

醉鱼草内生细菌ZJ1的抑菌作用及其脂肽类抑菌代谢产物鉴定

任璐, 刘晓峰, 周建波, 殷辉, 张宝俊, 赵晓军

山西农业大学植物保护学院, 山西太原 030031

通讯作者: 赵晓军
作者简介:任璐(1982-),女,山西汾阳人,副教授,博士,主要从事病原菌抗药性及生物农药的研究,E-mail:renlubaby@163.com
基金资助:
山西省应用基础研究计划项目（201801D121244）；山西省重点研发计划项目（201803D221005-7）资助

Abstract

Abstract: To identify antifungal action of Endophytic Bacteria ZJ1 from Buddleja lindleyana Fortune and its antimicrobial metabolites qualitative composition,the tablet confrontation and growth rate method were used to determine the antifungal action of ZJ1 strain and its fermentation broth to 10 kinds of pathogenic fungi. Medium pressure chromatography was used to isolate and purify the crude extracts of antifungal substances from ZJ1 strain,and LC-MS was used to identify the crude extracts by mass spectrometry. The growth status and membrane permeability of B. cinerea mycelia treated with ZJ1 fermentation broth were measured by microscopic observation and electrical conductivity method. The results showed that ZJ1 strain had certain inhibition to all the 10 fungal pathogens,among them,the inhibition rate of ZJ1 strain and its fermentation broth to M. fructicola were highest,reached 95.69% and 94.68%. The physical characteristics of metabolites of ZJ1 strain were measured and it was found that the metabolites of ZJ1 strain had the ability of discharging oil and the droplet collapse phenomenon occurred. Further tests showed that the metabolites of lipids in ZJ1 strain contained surfactant and ubiquitin. With B. cinerea as indicator pathogenic fungi treated with metabolites of ZJ1 strain,B. cinerea mycelia rarely sporulated,grew abnormally and fractured and the membrane permeability of B. cinerea increased,the permeable material flowed out,the electrical conductivity increased. The endophytic bacteria ZJ1 showed superior antifungal effect,and the identification of its peptide metabolites provided the basis for the further development and utilization of ZJ1 strain.

Key words: Buddleja lindleyana Fortune, Endophytic Bacteria, Antagonistic lipopeptide, Surfactin, Fengyic

摘要： 为明确醉鱼草(Buddleja lindleyana Fortune)内生细菌ZJ1的抑菌作用及其抑菌代谢物质组成，本研究测定了ZJ1菌株及其发酵液的抑菌作用，分离、纯化及鉴定了ZJ1菌株的抗菌物质，还观察和测定了ZJ1菌株代谢物处理后的Botrytis cinerea菌丝生长状态和细胞膜通透性。结果表明:ZJ1菌株对10种病原菌均有一定抑制作用，其中，活菌及发酵液均对Monilinia fructicola的抑制率最高，分别达95.69%和94.68%；观察ZJ1菌株代谢物的物理特性发现，其代谢物具有排油能力且出现液滴坍塌现象，进一步检测发现ZJ1菌株脂肽类代谢产物中含有表面活性素和泛革素；以B.cinerea作为指示菌，经ZJ1菌株代谢物处理后，B. cinerea菌丝极少产孢，生长异常，发生断裂；细胞膜通透性增大，渗透物质流出，电导率上升。醉鱼草内生细菌ZJ1表现出良好的抑菌作用，其脂肽类代谢产物的鉴定为ZJ1菌株的进一步开发和利用鉴定了基础。

关键词: 醉鱼草, 内生细菌, 抑菌脂肽, 表面活性素, 泛革素

CLC Number:

S482.7

REN Lu, LIU Xiao-feng, ZHOU Jian-bo, YIN Hui, ZHANG Bao-jun, ZHAO Xiao-jun. Antagonism of Endophytic Bacteria ZJ1 from Buddleja lindleyana Fortune and Identification of Antifungal Lipopetide Metabolites[J]. Acta Agrestia Sinica, 2021, 29(3): 434-442.

任璐, 刘晓峰, 周建波, 殷辉, 张宝俊, 赵晓军. 醉鱼草内生细菌ZJ1的抑菌作用及其脂肽类抑菌代谢产物鉴定[J]. 草地学报, 2021, 29(3): 434-442.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2021.03.003

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2021/V29/I3/434

References

[1] 李元,王子戌,李淑艳,等. 醉鱼草对鲫麻醉效果及血清生化指标的影响[J]. 大连海洋大学学报,2020,35(4):491-495
[2] 张伟,许双旺,吴德玲,等. 醉鱼草果实水部位化学成分及神经保护活性研究[J]. 天然产物研究与开发,2019,31(8):1397-1401
[3] 张洁. 醉鱼草属植物有效生物活性成分及农用杀虫活性研究进展[J]. 现代农业科技,2019(23):120-121
[4] 邓振山,李买平,郝雷,等. 巨菌草内生细菌多样性及其促生特性[J]. 草地学报,2019,27(5):1213-1221
[5] 魏立娟,王玉琴,杨成德,等. 青海省海晏县线叶嵩草内生细菌的生物功能鉴定及测定[J]. 草地学报,2018,26(3):764-769
[6] 陈中义,张杰,黄大昉. 植物病害生防芽胞杆菌抗菌机制与遗传改良研究[J]. 植物病理学报,2003,33(2):97-103
[7] 叶云峰,黎起秦,袁高庆,等. 枯草芽孢杆菌B47菌株抗菌物质的分离纯化及其对玉米小斑病的防治作用[J]. 中国生物防治学报,2011,27(3):357-361
[8] Ongena M,Jacques P. Bacillus Lipopeptides:Versatile Weapons for Plant Disease Biocontrol[J]. Trends in Microbiology,2008,16(3):115-125
[9] Asari S,Ongena M,Debois D,et al. Insights Into the Molecular Basis of Biocontrol of Brassica Pathogens by Bacillus Amyloliquefaciens UCMB5113 Lipopeptides[J]. Annals of Botany,2017,120(4):551-562
[10] 汪远,詹儒林,何红,等. 红树内生细菌菌株Kc-38的抗菌物质及对采后芒果炭疽病的防效[J]. 中国生物防治学报,2011,27(1):82-87
[11] Liu B,Huang L,Buchenauer H,et al. Isolation and Partial Characterization of an Antifungal Protein From the Endophytic Bacillus Subtilis Strain EDR4[J]. Pesticide Biochemistry and Physiology,2010,98(2):305-311
[12] Il Kim P,Chung K. Production of an Antifungal Protein for Control of Colletotrichum Lagenarium by Bacillus Amyloliquefaciens MET0908[J]. FEMS Microbiology Letters,2004,234(1):177-183
[13] 韩松,张守村,黄晓艳,等. 一株产铁载体内生细菌对尖孢镰刀菌的拮抗作用[J]. 西北植物学报,2011,31(5):1039-1044
[14] 夏启玉,王宇光,孙建波,等. 一株拮抗香蕉枯萎病的内生细菌的分离及其几丁质酶基因信号肽的分泌活性分析[J].中国生物工程杂志,2010,30(9):24-30
[15] Wang K,Yan P,Ding Q,et al. Diversity of culturable root-associated/endophytic bacteria and their chitinolytic and aflatoxin inhibition activity of peanut plant in China.[J].World journal of microbiology & biotechnology,2013,29(1):1-10
[16] 邓振山,何园,李超. 洋葱内生菌的筛选及其次级代谢产物分析[J]. 湖南农业大学学报(自然科学版),2016,(3):268-273
[17] 杨小帆,宋丽菊,齐盼盼,等. Pyrrosia Petiolosa内生细菌筛选及其抑菌活性物质分析[J].中国测试,2016,42(7):47-52
[18] 高振峰. 梓树内生细菌ZSYB-1分离鉴定及抗菌活性研究[D]. 太谷:山西农业大学,2015:33-34，44-46
[19] 李乐溪,李丹,张亮,等. 大蒜内生菌的分离及拮抗菌株的筛选与鉴定[J]. 江苏农业科学,2018(5):97-101
[20] 李玲玲,李青爱,易回香,等. 银杏内生菌的分离及其抑菌活性筛选[J]. 生物资源,2019(3):249-254
[21] 陈奕鹏,杨扬,桑建伟,等. 拮抗内生芽孢杆菌BEB17分离鉴定及其挥发性物质抑菌活性分析[J]. 植物病理学报,2018,48(4):537-546
[22] 蒋晶晶,王春明,陈明,等. 内生拮抗细菌X1-6-1的鉴定、发酵条件优化及抑菌作用研究[J]. 甘肃农业大学学报,2020,55(3):113-120
[23] 黄华毅,王佳琳,马荣,等. 枯草芽孢杆菌STO-12抑菌活性及其抑菌物质分析[J]. 浙江农业学报,2017,29(1):81-88
[24] 杨柯. 海芦笋内生细菌YK-HLS-11次级代谢产物研究[D]. 南京:南京农业大学,2017:50-51
[25] 李旭,马福海,王秀娟,等. 大豆根腐病生防菌KJB04-11的鉴定及其产生的脂肽类抗生素[J]. 生态学杂志,2012,31(6):1453-1460
[26] 王若琳,徐伟芳,王飞,等. 桑树内生拮抗菌的分离鉴定及其对桑断枝烂叶病的生防初探[J]. 微生物学报,2019,59(11):2130-2143
[27] 周金伟,周红丽,易有金,等. 短短芽胞杆菌对植物病原真菌的抑菌活性和抑菌机理[J]. 植物保护学报,2016,43(4):600-607
[28] 宁爽. 内生假单胞菌BTa14,Bar25促生抗病作用及机理的研究[D]. 烟台:烟台大学,2019:64
[29] 张丽,纪明山,谷祖敏,等. 印楝内生放线菌鉴定及对稻瘟病菌拮抗作用研究[J]. 中国生物防治学报,2014,30(4):534-539
[30] 王翀,刘薇,丁声,等. 戊唑醇对油菜菌核病菌作用机制的探讨[J]. 中国油料作物学报,2010,32(3):436-440
[31] 陈忠军,李海瑄,高鹤尘. 乳杆菌产生的抑菌活性物质对酵母细胞膜通透性的影响[J]. 中国乳品工业,2015,43(2):28-31
[32] 石玉星. 曼陀罗内生细菌MY1的初步鉴定及对谷瘟病抑菌作用研究[D]. 太谷:山西农业大学,2018:37-38

Antagonism of Endophytic Bacteria ZJ1 from Buddleja lindleyana Fortune and Identification of Antifungal Lipopetide Metabolites

醉鱼草内生细菌ZJ1的抑菌作用及其脂肽类抑菌代谢产物鉴定

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 7

Recommended Articles

Metrics

Comments

[1]	DENG Zhen-Shan, LI Mai-ping, HAO Lei, CHEN Kai-kai, LI Jing, LIU Yu-zhen, ZHANG Bao-bao, QI Xiang-ying. Diversity and Plant Growth Promoting Activities of the Culturable Endophytic Bacteria Associated with Pennisetum sp. [J]. Acta Agrestia Sinica, 2019, 27(5): 1213-1221.
[2]	WEI Li-juan, WANG Yu-qin, YANG Chen-de, XUE Li. Biological Function Identification and Determination of Endophytic Bacteria in Leaves of Kobresia capillifolia in Haiyan County, Qinghai Province [J]. Acta Agrestia Sinica, 2018, 26(3): 764-769.
[3]	CUI Yue-zhen, WAN Zhi-wen, FENG Jiang-rong, XUE Li, YANG Cheng-de. Research on IAA Secreting Ability of Endophytic Bacteria from Forage on Eastern-Qilian Mountain [J]. Acta Agrestia Sinica, 2016, 24(3): 618-623.
[4]	YAO Yu-ling, WANG Ying, WANG Yu-qin, YANG Cheng-de. Identification of Endophytic Bacteria from Kobresia humilis and Determination of Phosphate-solubilizing, IAA Secretion and Antagonistic Abilities [J]. Acta Agrestia Sinica, 2014, 22(6): 1252-1257.
[5]	WANG Yu-qin, XUE Li, YANG Cheng-de, WANG Ying, YAO Yu-ling, CHEN Xiu-rong. Identification and Determination of Biological Characteristics of Endophytic Bacteria 265ZY3 from Stipa capillata in the Alpine Grasslands [J]. Acta Agrestia Sinica, 2014, 22(4): 822-827.
[6]	LI Zhen-dong, CHEN Xiu-rong, YANG Cheng-de, LI Peng. Determination of the Capacity to Antagonistic towards Pathogenic Fungi and Identification of Anaphalis lactea Endophytic Bacteria RC14 [J]. , 2012, 20(4): 753-758.
[7]	LI Zhen-dong, CHEN Xiu-rong, LI Peng, MAN Bai-ying, WANG chen-Yue, ZOU Yu-kun, Xu Chang-lin. Identification of endogenous nitrogen-fixing bacteria of Polygonum viviparum and analysis on nitrogen fixation gene(nifH) [J]. , 2009, 17(5): 552-557.