Effects of Saline-alkali Stress on Photosynthesis Characteristics and Antioxidant System in Quinoa Seedlings Leaf

doi:10.11733/j.issn.1007-0435.2021.08.011

Acta Agrestia Sinica ›› 2021, Vol. 29 ›› Issue (8): 1689-1696.DOI: 10.11733/j.issn.1007-0435.2021.08.011

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Effects of Saline-alkali Stress on Photosynthesis Characteristics and Antioxidant System in Quinoa Seedlings Leaf

WANG Bao-qiang^1,2,3, ZHAO Ying^1,2,3, ZHU Xiao-lin^1,2,3, WANG Wang-tian^1,2,3, WEI Xiao-hong^1,2,3

1. College of Life Science and Technology, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China;
2. Gansu Key Lab of Crop Genetic &Germplasm Enhancement, Lanzhou, Gansu Province 730070, China;
3. Gansu Provincial Key Lab of Aridland Crop Science, Lanzhou, Gansu Province 730070, China

Received:2021-04-15 Revised:2021-06-10 Online:2021-08-15 Published:2021-09-06

盐碱胁迫对藜麦幼苗叶片光合特性及抗氧化系统的影响

王宝强^1,2,3, 赵颖^1,2,3, 朱晓林^1,2,3, 王旺田^1,2,3, 魏小红^1,2,3

1. 甘肃农业大学生命科学技术学院, 甘肃兰州 730070;
2. 甘肃省作物遗传改良与种质创新重点实验室, 甘肃兰州 730070;
3. 甘肃省干旱生境作物学重点实验室, 甘肃兰州 730070

通讯作者: 魏小红,E-mail:weixh@gsau.edu.cn
作者简介:王宝强(1984-),男,汉族,甘肃庆阳人,讲师,博士研究生,主要从事植物生态生理研究,E-mail:wangbq@gsau.edu.cn
基金资助:
国家自然科学基金项目（32060401）；甘肃农业大学青年导师扶持基金项目（GAU-QDFC-2018-12）资助

Abstract

Abstract: The aim of this study is to explore the effects of alkaline salt ratio and stress time on photosynthesis and antioxidant capacity of quinoa seedlings under mixed saline-alkali stress. We used 150 mmol·L^-1 saline-alkali mixed solution to treat quinoa seeds, and the changes of photosynthetic characteristics, malondialdehyde content and antioxidant enzyme activities were analyzed. The results showed that with the increase of the alkaline salt ratio and stress time, the chlorophyll content of quinoa leaves in each saline-alkali stress treatment group increased first and then decreased;The Net photosynthetic rate, Stomatal conductivity, Intercellular CO₂ concentration of quinoa seedling leaves all significantly decreased, and Stomatal limitation increased significantly. The content of malondialdehyde in leaves was highest in high ratio of alkaline salt treatment. The activities of superoxide dismutase, catalase and ascorbate peroxidase gradually decreased in the leaves, and first increased and then decreased in the stem;the peroxidase activity gradually decreased in the leaves, and the activity of the stem first decreased and then increased. The results indicated that the ratio of alkaline salt and stress time had adverse effects on the photosynthetic system and antioxidant system of quinoa seedlings leaves, but the synergistic effect of balanced ion ratio could improve the saline-alkali tolerance of quinoa seedlings. This study can provide scientific basis for the introduction and cultivation of quinoa in saline-alkali area and the biological improvement of saline-alkali land.

Key words: Quinoa, Salt-alkaline stress, Photosynthesis, Anti-oxidative enzymes

摘要： 为探究混合盐碱胁迫下碱性盐比例及胁迫时间对藜麦（Chenopodium quinoa）幼苗光合作用及抗氧化能力的影响，本研究以藜麦种子为材料，用150 mmol·L^-1盐碱混合溶液处理藜麦幼苗，分析其光合特征、丙二醛含量及抗氧化酶活性的变化。结果表明：随碱性盐比例及胁迫时间的增加，各盐碱胁迫处理组藜麦叶片叶绿素含量先升后降；各盐碱胁迫处理净光合速率、气孔导度、胞间CO₂浓度均显著低于对照，气孔限制值显著高于对照；叶中丙二醛含量在高比例碱性盐处理下达到最高；随胁迫时间的增加，叶片超氧化物歧化酶、过氧化氢酶及抗坏血酸过氧化物酶活性逐渐下降，茎中先升后降，叶中过氧化物酶活性逐渐下降，茎中先降后升。说明碱性盐比例及胁迫时间对藜麦幼苗叶片光合系统及抗氧化系统有不利影响。但均衡离子比例的协同效应可以改善藜麦幼苗的盐碱耐受性。本研究可为盐碱区域藜麦引种、栽培及盐碱地的生物改良提供科学依据。

关键词: 藜麦, 盐碱胁迫, 光合作用, 抗氧化酶活性

CLC Number:

S519

WANG Bao-qiang, ZHAO Ying, ZHU Xiao-lin, WANG Wang-tian, WEI Xiao-hong. Effects of Saline-alkali Stress on Photosynthesis Characteristics and Antioxidant System in Quinoa Seedlings Leaf[J]. Acta Agrestia Sinica, 2021, 29(8): 1689-1696.

王宝强, 赵颖, 朱晓林, 王旺田, 魏小红. 盐碱胁迫对藜麦幼苗叶片光合特性及抗氧化系统的影响[J]. 草地学报, 2021, 29(8): 1689-1696.

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Effects of Saline-alkali Stress on Photosynthesis Characteristics and Antioxidant System in Quinoa Seedlings Leaf

盐碱胁迫对藜麦幼苗叶片光合特性及抗氧化系统的影响

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