Responses of Rhizosphere Fungal Communities of Two Introduced Gramineous Grasses to Organic Fertilizer in the Qinghai Lake Region

doi:10.11733/j.issn.1007-0435.2022.10.011

Acta Agrestia Sinica ›› 2022, Vol. 30 ›› Issue (10): 2617-2625.DOI: 10.11733/j.issn.1007-0435.2022.10.011

Responses of Rhizosphere Fungal Communities of Two Introduced Gramineous Grasses to Organic Fertilizer in the Qinghai Lake Region

MA Kun¹, ZHAO Yang-an¹, ZHOU Xue-li^1,2, WANG Ying-cheng¹, JIN Xin¹, ZHANG Hai-juan¹, YAN Hui-lin¹, ZHANG Zhuo-hang¹, QIU Peng-ying¹, LU Guang-xin¹

1. College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai Province 810016, China;
2. Qinghai Province Grassland Improvement Experimental Station, Gonghe, Qinghai Province 813000, China

Received:2022-04-20 Revised:2022-06-07 Published:2022-11-05

环青海湖地区两种引种禾本科牧草根际真菌群落对有机肥的响应

马坤¹, 赵阳安¹, 周学丽^1,2, 王英成¹, 金鑫¹, 张海娟¹, 颜珲璘¹, 张卓航¹, 邱鹏滢¹, 芦光新¹

1. 青海大学农牧学院, 青海西宁 810016;
2. 青海省草原改良试验站, 青海共和 813000

通讯作者: 李建宏,E-mail:lugx74@qq.com
作者简介:马坤(1997-),男,甘肃定西人,硕士研究生,主要从事高寒草地微生物多样性及功能利用研究,E-mail:2314428497@qq.com
基金资助:
青海省科技厅基础研究面上计划项目(2021-ZJ-915);青海省科技厅成果转化专项“朱/豆混播的微生物调控及菌肥研发”(2022-SF-147)项目；2021年度青海省混合英才“高端创业人才”计划项目资助

Abstract

Abstract: In this study,two species of grasses introduced in the Qinghai Lake area, Elymus sibiricus 'Chuancao No.2'and Elymus nutans 'Aba',were used in the field trials to analyze the changes in growth and rhizosphere fungal community structure after organic fertilization using ITS rDNA Illumina high-throughput sequencing technology and molecular ecological network. The results showed that organic fertilizer significantly increased the above- and below-ground biomass and decreased the stem-to-leaf ratio of both forages compared with the control (P<0.05);organic fertilizer treatment changed the soil physicochemical properties,increased the content of total nitrogen (TN) and organic carbon (SOC) (P<0.05),and decreased the soil pH. The redundancy analysis (RDA) structure showed that pH and SOC were the main environmental factors driving microbial changes (P<0.01),and organic fertilizer addition reconstructed the network relationships among soil fungal communities. Short-term application of organic fertilizer in alpine areas significantly promoted forage growth,improved soil fertility,and changed microbial community structure.

Key words: Organic fertilizer, Fungal community structure, High-throughput sequencing, Network Symbiosis

摘要： 本研究以环青海湖地区引进的两种禾本科牧草'川草2号’老芒麦(Elymus sibiricus 'Chuancao No.2’)和'阿坝’垂穗披碱草(Elymus nutans 'Aba’)为研究对象，通过田间试验，采用ITS rDNA Illumina高通量测序技术和分子生态网络的方法，分析施用有机肥后两种牧草生长及根际真菌群落结构变化。结果显示，与对照相比，有机肥显著增加两种牧草的地上及地下生物量，降低牧草茎叶比(P<0.05)；有机肥处理改变了土壤理化性质，提高全氮(TN)和有机碳(SOC)含量(P<0.05)，降低土壤pH。土壤真菌群落结构表明：有机肥降低老芒麦根际主要优势菌和次要优势菌的相对丰度；提高披碱草根际赤霉菌(Gibberella)、内生真菌属(Preussia)相对丰度。RDA结构显示pH和SOC是驱动微生物变化的主要环境因子(P<0.01)，有机肥添加重新构建了土壤真菌群落间的网络关系。高寒地区短期施加有机肥明显促进牧草生长，提高土壤肥力，改变微生物群落结构。

关键词: 有机肥, 真菌群落结构, 高通量测序, 网络共生关系

CLC Number:

S154.32

MA Kun, ZHAO Yang-an, ZHOU Xue-li, WANG Ying-cheng, JIN Xin, ZHANG Hai-juan, YAN Hui-lin, ZHANG Zhuo-hang, QIU Peng-ying, LU Guang-xin. Responses of Rhizosphere Fungal Communities of Two Introduced Gramineous Grasses to Organic Fertilizer in the Qinghai Lake Region[J]. Acta Agrestia Sinica, 2022, 30(10): 2617-2625.

马坤, 赵阳安, 周学丽, 王英成, 金鑫, 张海娟, 颜珲璘, 张卓航, 邱鹏滢, 芦光新. 环青海湖地区两种引种禾本科牧草根际真菌群落对有机肥的响应[J]. 草地学报, 2022, 30(10): 2617-2625.

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Responses of Rhizosphere Fungal Communities of Two Introduced Gramineous Grasses to Organic Fertilizer in the Qinghai Lake Region

环青海湖地区两种引种禾本科牧草根际真菌群落对有机肥的响应

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