Effect of Complex Microorganisms on the Growth and Physiological Characteristics of Lolium multiflorum Seedlings under Tetracycline Stress

doi:10.11733/j.issn.1007-0435.2022.11.017

Acta Agrestia Sinica ›› 2022, Vol. 30 ›› Issue (11): 3009-3017.DOI: 10.11733/j.issn.1007-0435.2022.11.017

Effect of Complex Microorganisms on the Growth and Physiological Characteristics of Lolium multiflorum Seedlings under Tetracycline Stress

ZHANG Chen^1,2, LI Chang-ning^1,2, YAO Tuo^1,2, HE Li^1,2, CHEN Xin^1,2, ZHANG Lan^1,2, XI Bo-ai^1,2

1. College of Grassland Science, Gansu Agricultural University, Lanzhou, Gansu Province 730070;
2. Key Laboratory of Grassland Ecosystem, Gansu Agricultural University, Lanzhou, Gansu Province 730070

Received:2022-05-13 Revised:2022-08-15 Published:2022-12-02

复合微生物对四环素胁迫下多花黑麦草苗期生长和生理特性的影响

张琛^1,2, 李昌宁^1,2, 姚拓^1,2, 何礼^1,2, 陈鑫^1,2, 张澜^1,2, 席博爱^1,2

1. 甘肃农业大学草业学院, 甘肃兰州 730070;
2. 草业生态系统教育部重点实验室, 甘肃兰州 730070

通讯作者: 姚拓,E-mail:yaotuo@gsau.edu.cn
作者简介:张琛(1999-),女,汉族,山西晋中人,硕士研究生,主要从事草地微生物多样性研究,Email:1073973814@qq.com;
基金资助:
甘肃省教育厅双一流重大科研项目(No.GSSYLXM-02)资助

Abstract

Abstract: In order to investigate the effect of complex microbial agents on Lolium multiflorum under abiotic stress,Lolium multiflorum was subjected to different concentrations (0,50,100,150 mg·L^-1) of tetracycline stress as the study material to investigate the effect of the addition of complex microbial agents (MI) on the growth and physiological characteristics of Lolium multiflorum under tetracycline stress. The results showed that the effect of MI addition on the growth and physiological characteristics of Lolium multiflorum was not significant. The results showed that the addition of MI increased the root and shoot lengths,increased chlorophyll content,root height,and above-ground biomass,improved root tip cell number and morphology,improved root traits,increased antioxidant enzymes (SOD,POD,and CAT) activities,and decreased relative cell conductivity and malondialdehyde (MDA) content of Lolium multiflorum seedlings under tetracycline stress. The results of the above study showed that the addition of complex microorganisms enhanced the degradation of tetracycline in Lolium multiflorum,and the bacteriophage promoted the growth and development of Lolium multiflorum under tetracycline stress and repaired the cell damage caused by tetracycline stress,and the addition of bacteriophage improved the resistance of Lolium multiflorum to tetracycline stress.

Key words: Composite microorganism, Lolium multiflorum, Tetracycline degradation, Growth characteristics, Physiological property, Plant-microbial

摘要： 为探究复合微生物菌剂对非生物胁迫下多花黑麦草(Lolium multiflorum)的作用效果，本试验以不同浓度(0，50，100，150mg·L^-1)四环素胁迫下多花黑麦草为研究材料，探讨四环素胁迫下添加复合微生物菌剂(MI)对多花黑麦草生长和生理特性的影响。结果表明：添加菌剂(MI)能够增加四环素胁迫下多花黑麦草幼苗根长芽长，增加叶绿素含量、株高根长及地上地下生物量，改善根尖细胞数量及形态，改善根系性状，增加抗氧化酶(SOD,POD及CAT)活性，降低细胞相对电导率及丙二醛(MDA)含量。因此，添加复合微生物强化了多花黑麦草对四环素的降解作用，菌剂对四环素胁迫下多花黑麦草的生长发育具有促进作用，并对四环素胁迫造成的细胞损伤具有修复作用，菌剂的添加提高了多花黑麦草的抗四环素胁迫能力。

关键词: 复合微生物, 多花黑麦草, 四环素降解, 生理特性, 生长特性, 植物-微生物

CLC Number:

X53
S154.3

ZHANG Chen, LI Chang-ning, YAO Tuo, HE Li, CHEN Xin, ZHANG Lan, XI Bo-ai. Effect of Complex Microorganisms on the Growth and Physiological Characteristics of Lolium multiflorum Seedlings under Tetracycline Stress[J]. Acta Agrestia Sinica, 2022, 30(11): 3009-3017.

张琛, 李昌宁, 姚拓, 何礼, 陈鑫, 张澜, 席博爱. 复合微生物对四环素胁迫下多花黑麦草苗期生长和生理特性的影响[J]. 草地学报, 2022, 30(11): 3009-3017.

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Effect of Complex Microorganisms on the Growth and Physiological Characteristics of Lolium multiflorum Seedlings under Tetracycline Stress

复合微生物对四环素胁迫下多花黑麦草苗期生长和生理特性的影响

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