Responses of N2O flux to short-term nitrogen and phosphorus addition in grassland ecosystem of agro-pastoral ecotone

doi:10.11733/j.issn.1007-0435.2022.12.004

Acta Agrestia Sinica ›› 2022, Vol. 30 ›› Issue (12): 3199-3206.DOI: 10.11733/j.issn.1007-0435.2022.12.004

Responses of N₂O flux to short-term nitrogen and phosphorus addition in grassland ecosystem of agro-pastoral ecotone

YAN Xue-dong^1,2,3, CHEN Xiao-peng^1,2,3, HAO Jie^1,2,3, DIAO Hua-jie^1,2,3, LI Hongyu^2,4, DONG Kuan-hu^1,2,3, WANG Chang-hui^1,2,3

1. College of Grassland Science, Shanxi Agricultural University, Taigu, Shanxi 030801, China;
2. Shanxi Key Laboratory of Grassland Ecological Protection and Native Grass Germplasm Innovation, Taigu, Shanxi 030801, China;
3. Youyu Loess Plateau Grassland Ecosystem Research Station, Youyu, Shanxi 037200, China;
4. Office of Research Administration, Shanxi Agricultural University, Taigu, Shanxi 030801, China

Received:2022-06-05 Revised:2022-08-31 Published:2023-01-04

农牧交错带草地生态系统N₂O通量对短期氮、磷添加的响应

燕学东^1,2,3, 陈晓鹏^1,2,3, 郝杰^1,2,3, 刁华杰^1,2,3, 李红玉^2,4, 董宽虎^1,2,3, 王常慧^1,2,3

1. 山西农业大学草业学院, 山西太谷 030801;
2. 草地生态保护与乡土草种质创新山西省重点实验室, 山西太谷 030801;
3. 山西右玉黄土高原草地生态系统国家定位观测研究站, 山西右玉 037200;
4. 山西农业大学科研管理部, 山西太谷 030801

通讯作者: 董宽虎,E-mail:dongkuanhu@126.com;王常慧,E-mail:wangch@ibcas.ac.cn
作者简介:燕学东(1995-),男,汉族,山西晋中人,硕士研究生,主要从事草地生态与管理研究,E-mail:yxd13111039753@163.com
基金资助:
山西农业大学高层次引进人才项目资助(2021XG008),国家重点研发计划(2017YFA0604802)和山西省重点实验室项目(202104010910017)资助

Abstract

Abstract: Nitrogen (N) and phosphorus (P) are two important nutrients for maintaining the productivity of terrestrial ecosystems. To investigate how P addition and the interaction of N and P affect N₂O flux and regulatory mechanisms of grassland ecosystems in agro-pastoral ecotone,four treatments (CK:control,N:nitrogen addition (N:urea 10 g·m^-2·a^-1),P:phosphorus addition (calcium dihydrogen phosphate 10 g·m^-2·a^-1),NP:(simultaneous addition of nitrogen and phosphorus) were set up in this study. The N₂O flux were measured by static dark box-gas chromatography in the growing seasons of 2018 and 2019,and the effects of N,P,and their interaction on N₂O fluxes were investigated by structural equation model (SEM). The results showed that N addition significantly reduced the N₂O emission in the 2018 growing season,while P addition significantly reduced the N₂O emission in both 2018 and 2019 growing seasons,and the simultaneous addition of N and P weakened the inhibitory effect of single nitrogen or phosphorus addition on N₂O emission;NO^-₃-N,NH⁺₄-N and soil moisture content were the main factors that affected N₂O flux. In total,there was a significant interaction between N and P addition on N₂O flux of grassland in the agro-pastoral ecotone,and it was regulated by interannual temperature and precipitation. The change of precipitation patterns should be considered in the study of the effect of nutrient management on N₂O flux in the agro-pastoral ecotone.

Key words: Nutrient addition, Agro-pastoral ecotone, N₂O emission, Greenhouse gases

摘要： 氮(N)、磷(P)是维持陆地生态系统生产力的重要营养元素，为探求磷添加和氮、磷的交互作用如何影响农牧交错带草地生态系统N₂O通量及调控机制，本研究设置对照(CK)、氮添加(N：缓释尿素10g·m^-2·a^-1)、磷添加(P：磷酸二氢钙10g·m^-2·a^-1)以及氮、磷同时添加(NP)4个处理，通过静态暗箱-气相色谱法测定2018和2019年生长季N₂O通量，通过结构方程模型等寻求氮、磷及其互作效应对N₂O通量的影响。结果表明：氮添加显著降低了2018年生长季N₂O排放，而磷添加显著降低了两年生长季N₂O排放；氮、磷同时添加削弱了单独氮或磷添加对N₂O排放的抑制作用；硝态氮、铵态氮和土壤含水量是N₂O通量的主要影响因素。因此，氮、磷添加对农牧交错带草地N₂O通量的影响存在显著的交互作用，并且受年际间温度和降水的调控，农牧交错带草地养分管理对N₂O通量的影响应考虑降水格局的变化。

关键词: 养分添加, 农牧交错带, N₂O排放, 温室气体

CLC Number:

S812

YAN Xue-dong, CHEN Xiao-peng, HAO Jie, DIAO Hua-jie, LI Hongyu, DONG Kuan-hu, WANG Chang-hui. Responses of N₂O flux to short-term nitrogen and phosphorus addition in grassland ecosystem of agro-pastoral ecotone[J]. Acta Agrestia Sinica, 2022, 30(12): 3199-3206.

燕学东, 陈晓鹏, 郝杰, 刁华杰, 李红玉, 董宽虎, 王常慧. 农牧交错带草地生态系统N₂O通量对短期氮、磷添加的响应[J]. 草地学报, 2022, 30(12): 3199-3206.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2022.12.004

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2022/V30/I12/3199

References

[1] 王彩绒,田霄鸿,李生秀. 土壤中氧化亚氮的产生及减少排放量的措施[J]. 生态环境学报,2001,10(2):143-148
[2] WANG Z Q. Chinese salinized soil[J]. Science Press,1993(1):363-364
[3] 陈先江,王彦荣,侯扶江. 草地生态系统温室气体排放机理及影响因素[J]. 草业科学,2011,28(5):722-728
[4] TIAN H Q,XU R T,CANADELL J G,et al. A comprehensive quantification of global nitrous oxide sources and sinks[J]. Nature,2020,586(7828):248
[5] 赵哈林,周瑞莲,张铜会,等. 我国北方农牧交错带的草地植被类型、特征及其生态问题[J]. 中国草地,2003,25(3):8
[6] PEICHL M,LEAVA N A,KIELY G. Above-and belowground ecosystem biomass,carbon and nitrogen allocation in recently afforested grassland and adjacent intensively managed grassland[J]. Plant and Soil,2012,350(1-2):281-296
[7] 王敬国. 农业生态系统和大气间的温室效应气体交换[J]. 环境科学,1993(2):49-53
[8] 韩冰,耿依仪,邓艳芳，等. 氮添加对青藏高原高寒草甸土壤磷组分的影响[J/OL]. http://kns.cnki.net/kcms/detail/11.3362.S.20220825.1203.002.html,2022-08-25/2022-09-30
[9] Liu L L,Greaver Tara L. A review of nitrogen enrichment effects on three biogenic GHGs:the CO₂ sink may be largely offset by stimulated N₂O and CH₄ emission[J]. Ecology Letters,2009,12(10):1103-1117
[10] 孙英杰,吴昊王,亚楠. 硝化反硝化过程中N₂O释放影响因素[J]. 生态环境学报,2011,20(2):384-388
[11] WOLF I,RUSSOW R. Different pathways of formation of N₂O,N₂ and NO in black earth soil[J]. Soil Biology & Biochemistry,2000,32(2):229-239
[12] SHCHERBAK I,MILLAR N,ROBERTSON G P. Global metaanalysis of the nonlinear response of soil nitrous oxide (N₂O) emissions to fertilizer nitrogen[J]. Proceedings of the National Academy of Sciences,2014,111(25)37-43
[13] MA W,JIANG S,ASSEMIEN F,et al. Response of microbial functional groups involved in soil N cycle to N,P and NP fertilization in Tibetan alpine meadows[J]. Soil Biology and Biochemistry,2016,101(2):195-206
[14] YE R Z. Nitrous oxide uptake in rewetted wetlands with contrasting soil organic carbon contents[J]. Soil Biology and Biochemistry,2016,100(10):110-117
[15] YAN J C,XIAO D,et al. Potential short-term effects of yak and Tibetan sheep dung on greenhouse gas emissions in two alpine grassland soils under laboratory conditions[J]. Biology and fertility of soils:Cooperating Journal of the International Society of Soil Science,2013,49(8):11-17
[16] 秦加敏,王常慧,曹颖,等. 氮添加和刈割对内蒙古典型草原植被碳氮库的影响[J]. 草地学报,2022,30(1):12-20
[17] 杨建强,刁华杰,胡姝娅,等. 不同水平氮添加对盐渍化草地土壤微生物特征的影响[J]. 植物生态学报,2021,45(7):2307-2314
[18] 申颜,孙建平,罗玉坤,等. 短期放牧对半干旱草地生态系统CO₂和N₂O排放的影响[J]. 环境科学,2018,39(11):5237-5245
[19] 王岩,刁华杰,董宽虎,等. 降水变化与氮添加对晋北盐碱化草地土壤净氮矿化的影响[J]. 应用生态学报,2021,32(7):2389-2396
[20] 曹美秋,张桦. 气相色谱同时测定NO,CO,N₂O,CO₂[J]. 环境化学,1985(5):72-76
[21] 王迎红. 陆地生态系统温室气体排放观测方法研究、应用及结果比对分析[D].北京:中国科学院研究生院(大气物理研究所),2005:13-20
[22] 叶琼丹,任飞,李永慧,张子杨,樊娜,冶俊,李希来,李兰平. 不同养分添加对高寒植物功能性状的影响[J/OL]. http://kns.cnki.net/kcms/detail/11.3362.S.20220812.1352.018.html,2022-08-25/2022-09-30
[23] THOMPSON R L,LASSALETTA L,PATRA P K,et al. Acceleration of global N₂O emissions seen from two decades of atmospheric inversion[J]. Nature Climate Change,2019,9(2):156
[24] WANG F,LI J,WANG X L,et al. Nitrogen and phosphorus addition impact soil N₂O emission in a secondary tropical forest of South China[J]. Scientific Reports,2014,4(1):5615
[25] ABMAEL D S C,JULIANA B J,RICARADE R,ANA C R. How do greenhouse gas emissions vary with biofertilizer type and soil temperature and moisture in a tropical grassland?[J]. Pedosphere,2020,30(5):607-617
[26] LIU C,ZHANG Y S,Liu H,et al. Fertilizer stabilizers reduce nitrous oxide emissions from agricultural soil by targeting microbial nitrogen transformations[J]. Science of The Total Environment,2021,15(1):225
[27] 黄瑞灵,王西文,马国虎,等. 模拟氮沉降对高寒湿地土壤理化性质和酶活性的影响[J]. 草地学报,2022,30(6):1343-1349
[28] ZHAO Y,PETH S,RESZKOWSKA A,et al. Response of soil moisture and temperature to grazing intensity in a Leymus chinensis steppe,Inner Mongolia[J]. Plant and soil,2011,340(1-2):89-102
[29] XIA W W,BOWATTE S,JIA Z J,NEWTON P. Offsetting N₂O emissions through nitrifying CO₂ fixation in grassland soil[J]. Soil Biology and Biochemistry,2022,165(165):108-228
[30] PENG Y,WANG G,LI F,et al. Soil Temperature Dynamics Modulate N₂O Flux Response to Multiple Nitrogen Additions in an Alpine Steppe[J]. Journal of Geophysical Research,2018,123(12):1244-1344
[31] KAZI R M,FEIKE A. DIJKSTRA. Denitrification and associated N₂O emissions are limited by phosphorus availability in a grassland soil[J]. Geoderma,2016,284(284):32-45
[32] VANCE E D,BROOKES P C,JENKINSON D S. An extraction method for measuring soil microbial biomass C[J]. Soil Biology & Biochemistry,1987,19(6):703-707
[33] ZHANG J,HAN X. N₂O emission from the semi-arid ecosystem under mineral fertilizer (urea and superphosphate) and increased precipitation in northern China[J]. Atmospheric Environment,2008,42(2):291-302
[34] 曹登超,高霄鹏,李磊,等. 氮磷添加对昆仑山北坡高山草地N₂O排放的影响[J]. 植物生态学报,2019,43(2):165-173
[35] GOMEZ C N,HUDIBURG T W,BERNACCHI C J,PARTON W J,DELUCIA E H. Nitrogen deposition and greenhouse gas emissions from grasslands:uncertainties and future directions[J]. Global change biology,2016,22(4):323-334
[36] SHEN Y,ZHU B. Effects of nitrogen and phosphorus enrichment on soil N₂O emission from natural ecosystems:A global meta-analysis[J]. Environmental Pollution,2022,118(9):93
[37] 贺星,马文红,梁存柱,等. 养分添加对内蒙古不同草地生态系统生物量的影响[J]. 北京大学学报,2015，51(4),657-666

Responses of N₂O flux to short-term nitrogen and phosphorus addition in grassland ecosystem of agro-pastoral ecotone

农牧交错带草地生态系统N₂O通量对短期氮、磷添加的响应

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	YE Qiong-dan, REN Fei, LI Yong-hui, ZHANG Zi-yang, FAN Na, YE Jun, LI Xi-lai, LI Lan-ping. Effects of Different Nutrient Additions on Functional Traits of Alpine Plant [J]. Acta Agrestia Sinica, 2022, 30(10): 2737-2744.
[2]	LU Yu-lan, LI Guang, YAN Li-juan, DONG Li-xia, LI Jie, NIE Zhi-gang, WANG Jun. Study on the Change of Precipitation in Agro-Pastoral Ecotone of Gansu Province from 1971 to 2019 [J]. Acta Agrestia Sinica, 2021, 29(11): 2556-2565.
[3]	ZHOU Lei, WANG Lu, ZHAO Bao-ping, MI Jun-zhen, WANG Feng-wu, LIU Jing-hui, ZHANG Dan, LIU Ye-kun. Effect of Different Sowing Date and Cutting Time on Yield and Quality of Forage Oat in Agro-pastoral Ecotone of Northern China [J]. Acta Agrestia Sinica, 2021, 29(10): 2355-2363.
[4]	WANG Hui-ling, DIAO Hua-jie, CUI Le-le, BO Yuan-chao, LIU Xia-lin, REN Yu-jia, HAO Jie, LI Yang, SHEN Yan, WANG Chang-hui, DONG Kuan-hu. Response of Soil Respiration and Its Components to Mowing Intensities in a Typical Grassland in the Agro-pastoral Ecotone of Northern China [J]. Acta Agrestia Sinica, 2020, 28(5): 1403-1411.
[5]	DU Zhong-yu, AN Hui, WANG Bo, WEN Zhi-lin, ZHANG Ya-rou, WU Xiu-zhi, LI Qiao-ling. Effects of Nutrient Addition and Precipitation Manipulation on Plant Species Diversity and Biomass of in a Desert Grassland [J]. Acta Agrestia Sinica, 2020, 28(4): 1100-1110.
[6]	TAN Yan-ci, BAO Ya-jing, LI Zheng-hai, ZHANG Jing, LIANG Jie, LV Na, YE Jia-qi, LIU Chong, XU Yuan. Calorific Values of Grassland Plant Population and Functional Groups in the Agro-pastoral Ecotone of Inner Mongolia and Liaoning Province Border [J]. Acta Agrestia Sinica, 2019, 27(1): 15-21.
[7]	DONG Xiao-xiao, BO Yuan-chao, ZHANG Xiao-lin, HE Ting-ting, WANG Chang-hui, DONG Kuan-hu. Response of ecosystem soil respiration in semi-arid grassland to short-term grazing intensity in Agro-pastoral ecotone [J]. Acta Agrestia Sinica, 2019, 27(1): 53-62.
[8]	LIN Chang-cun, WANG Kun, SUN Yu-ming. Study on the Change of Temperature Time Series in Medium Section of Agro-pastoral Ecotone of Northern China During the Last 60 Years [J]. Acta Agrestia Sinica, 2016, 24(4): 747-753.
[9]	ZHAO Xiao-chen, HUANGFU Chao-he, LIU Hong-mei, XIU Wei-ming, ZHAO Jian-ning, YANG Dian-lin. Response of Soil Enzyme Activity and Microbial Biomass Carbon and Nitrogen to the Nutrient Addition of Stipa Baicalensis Steppe in Inner Mongolia [J]. Acta Agrestia Sinica, 2016, 24(1): 47-53.
[10]	CHEN Chao, HUANG Ding, WANG Kun, ZHANG Wei-hua, SUN Zeng-hui, CHEN Long. Distribution and Dispersal of Alien Sweet Clover along Roadside in An Agro-pastoral Ecotone of Northern China [J]. Acta Agrestia Sinica, 2014, 22(4): 722-727.
[11]	WEI Wen. Effects of Land Use Change on Environment in Agro-pastoral Ecotone Based on Ecosystem Services Value：A case study in Taipusi, Inner Mongolia [J]. , 2014, 22(2): 249-254.
[12]	WEI Wen, GAO Wa, SHI Shang-li, TIAN Qing-song, YU Feng-chun. Ecosystem Change Analysis of Agriculture and Animal Husbandary Regions in Last Ten Years:A Case Study in Taipusi,Inner Mongolia [J]. , 2012, 20(1): 31-36.
[13]	WEI Da, XU Ri, WANG Ying-hong, YAO Tan-dong. CH₄,N₂O and CO₂ Fluxes and Correlation with Environmental Factors of Alpine Steppe Grassland in Nam Co Region of Tibetan Plateau [J]. , 2011, 19(3): 412-419.
[14]	XU Xuan, ZHANG Ying-jun . IRM Approach to Analyze the Coupling of Pasture,Livestock and Crop Systems in the Agro-pastoral Ecotone [J]. , 2010, 18(3): 320-326.
[15]	Wang Gengchen, Du Rui, Liu Guangren, Wang Yanfen, Wang Yuesi, Chen Zuozhong, Lu Daren . Characteristics of N₂O and CH₄ Fluxes and Their Seasonal Variations From Inner Mongolia Grassland [J]. , 1998, 6(4): 306-311.