Effects of Applying Organic Fertilizer on Rhizosphere Microorganisms and Soil Physical and Chemical Properties of Elymus nutans

doi:10.11733/j.issn.1007-0435.2022.03.011

Acta Agrestia Sinica ›› 2022, Vol. 30 ›› Issue (3): 594-602.DOI: 10.11733/j.issn.1007-0435.2022.03.011

Effects of Applying Organic Fertilizer on Rhizosphere Microorganisms and Soil Physical and Chemical Properties of Elymus nutans

MA Kun¹, LU Guang-xin¹, DENG Ye², YAN Hui-lin¹, WANG Ying-cheng¹, ZHAO Yang-an¹, ZHANG Hai-juan¹, ZHOU Xue-li³, DOU Sheng-yun³

1. College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai Province 810016, China;
2. Research Center for Ecological Environment, Chinese Academy of Sciences, Beijing 100080, China;
3. Qinghai Province Grassland Improvement Experimental Station, Gonghe, Qinghai Province 813000, China

Received:2021-07-21 Revised:2021-08-29 Published:2022-03-30

有机肥对垂穗披碱草根际微生物及土壤理化性质的影响

马坤¹, 芦光新¹, 邓晔², 颜珲璘¹, 王英成¹, 赵阳安¹, 张海娟¹, 周学丽³, 窦声云³

1. 青海大学农牧学院, 青海西宁 810016;
2. 中国科学院生态环境研究中心, 北京 100080;
3. 青海省草原改良试验站, 青海共和 813000

通讯作者: 芦光新,E-mail:lugx74@qq.com;邓晔,E-mail:yedeng@rcees.ac.cn
作者简介:马坤(1997-),男,甘肃定西人,硕士研究生,主要从事高寒草地微生物多样性及功能利用研究,E-mail:2314428497@qq.com
基金资助:
青海省科技厅基础研究面上计划项目(2021-ZJ-915)；2021年度青海省“昆仑英才·高端创新创业人才”计划项目资助

Abstract

Abstract: To investigate the effects of organic fertilizer application rate on soil microbial community structure and physical and chemical properties of artificial grassland after planting,Elymus nutans ‘Aba’ was used as experimental material,and three treatments,no fertilizer (CK),50% organic fertilizer and 75% organic fertilizer,were set. The results showed that compared with CK,the total nitrogen and organic matter contents in the rhizosphere soil were increased and the pH value of the soil was decreased when organic fertilizer was applied. The composition of the soil rhizosphere microbial community was changed with the addition of organic fertilizer,and the relative abundance of dominant bacteria actinomycetes and basidiomycetes was increased with the addition of organic fertilizer at 50%. The relative abundance of Gibberellum and endophytic fungi were increased when organic fertilizer was 75%. The results of Beta diversity analysis showed that the bacterial and fungal community structures were significantly different between the control and each treatment. The results showed that increased application of organic fertilizer could improve soil rhizosphere microbial community structure,further improve soil health,and provide suitable soil microbial conditions for herbage growth in the alpine region.

Key words: Elymus nutans, Organic fertilizer, Soil physical and chemical properties, Rhizosphere microorganisms

摘要： 为了探究有机肥施肥量对人工草地建植后土壤微生物群落结构及理化性质的影响，以‘阿坝’垂穗披碱草(Elymus nutans ‘Aba’)为试验材料，设置不施肥(CK)，50%，75%有机肥三个处理，测定土壤理化性质及土壤微生物结构，结果表明：与CK相比，有机肥施用当年，提高牧草根际土壤总氮和有机质含量(P<0.05)，降低了土壤pH值(P<0.05)；有机肥的添加引起了土壤根际微生物群落组成的变化，有机肥施用量为50%时增加了优势菌放线菌门、担子菌门的相对丰度；有机肥施用量为75%时明显增加了赤霉菌属、内生真菌属的相对丰度；Beta多样性分析结果表明，对照和各处理细菌和真菌群落结构明显不同。本研究结果表明，增施有机肥能够改善土壤根际微生物群落结构，进一步改善土壤健康状况，为高寒地区牧草生长提供适宜的土壤微生物条件。

关键词: 垂穗披碱草, 有机肥, 土壤理化性质, 根际微生物

CLC Number:

S938.1

MA Kun, LU Guang-xin, DENG Ye, YAN Hui-lin, WANG Ying-cheng, ZHAO Yang-an, ZHANG Hai-juan, ZHOU Xue-li, DOU Sheng-yun. Effects of Applying Organic Fertilizer on Rhizosphere Microorganisms and Soil Physical and Chemical Properties of Elymus nutans[J]. Acta Agrestia Sinica, 2022, 30(3): 594-602.

马坤, 芦光新, 邓晔, 颜珲璘, 王英成, 赵阳安, 张海娟, 周学丽, 窦声云. 有机肥对垂穗披碱草根际微生物及土壤理化性质的影响[J]. 草地学报, 2022, 30(3): 594-602.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2022.03.011

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2022/V30/I3/594

References

[1] YOU Q G,XUE X,PENG F,et al. Comparison of ecosystem characteristics between degraded and intact alpine meadow in the Qinghai-Tibetan Plateau,China[J]. Ecological Engineering,2014,71(10):133-143
[2] 张骞,马丽,张中华,等. 青藏高寒区退化草地生态恢复:退化现状、恢复措施、效应与展望[J]. 生态学报,2019,39(20):7441-7451
[3] 周华坤,赵新全,周立,等. 青藏高原高寒草甸的植被退化与土壤退化特征研究[J]. 草业学报,2005(3):31-40
[4] 孙鸿烈,郑度,姚檀栋,等. 青藏高原国家生态安全屏障保护与建设[J]. 地理学报,2012,67(1):3-12
[5] 党永智,李欣,权欣,等. 施用有机肥对高寒草甸地上生物量及物种多样性的影响[J]. 青海畜牧兽医杂志,2015,45(6):4-6
[6] YANG Y D,WANG P X,ZENG Z H,et al. Dynamics of Bacterial Communities in a 30-Year Fertilized Paddy Field under Different Organic-Inorganic Fertilization Strategies[J]. Agronomy,2019,9(1):101-107
[7] 刘纪爱,束爱萍,刘光荣,等. 施肥影响土壤性状和微生物组的研究进展[J]. 生物技术通报,2019,35(9):21-28
[8] ZHAO C,ZHU L,LIANG J,et al. Effects of experimental warming and nitrogen fertilization on soil microbial communities and processes of two subalpine coniferous species in Eastern Tibetan Plateau,China[J]. Plant Soil,2014(382):189-201
[9] VANDER HEIJDEN M G A,KLIRONOMOS J N,URSIC M. My-corrhizal fungal diversity determines plant biodiversity,ecosystem variability and productivity[J]. Nature,1998,396(6706):69-72
[10] LAU J A,LENNON J T,HEATH K D,et al. Trees harness the power of microbes to survive climate change[J]. PNAS,2017,114(42):11009-11011
[11] SHI S,NUCCIO E,HENMAN DJ,et al. Successional trajectories of rhizosphere bacterial communities over consecutive seasons[J]. mBio,2015,6(4):76-87
[12] 仁增旺姆,姜丽丽,汪诗平,等.氮磷添加对垂穗披碱草人工草地生产生态功能的影响分析[J]. 高原科学研究,2020,4(3):55-61
[13] 张英俊,任继周,王明利,等. 论牧草产业在我国农业产业结构中的地位和发展布局[J]. 中国农业科技导报,2013,15(4):61-71
[14] 王晓芬. 光照、施肥及刈割对青藏高原三种牧草生长的影响[D]. 兰州:兰州大学,2008:20-34
[15] 刘杏兰,高宗,刘存寿,等. 有机—无机肥配施的增产效应及对土壤肥力影响的定位研究[J]. 土壤学报,1996(2):138-142,144-147
[16] 李二云. 土壤酸碱性对植物生长的影响及其改良措施[J]. 现代农村科技,2012(6):48
[17] SUN R,ZHANG X X,GUO X,et al. Bacterial diversity in soils subjected to long-term chemical fertilization can be more stably maintained with the addition of livestock manure than wheat straw[J]. Soil Biology and Biochemistry,2015(88):9-18
[18] SIX J,FREY S,THIET R,et al. Bacterial and fungal contributions to carbon sequestration in agroecosystems[J]. Soil Science Society of America Journal,2006,70(2):555-569
[19] RAMIREZ K S,CRAIN J M,FIERER N. Consistent effects of nitrogen amendments on soil microbial communities and processes across biomes[J]. Global Change Biology,2012,18(6):1918-1927
[20] 杨希智,王长庭,字洪标,等. 三江源区不同建植年限人工草地土壤微生物群落结构特征[J]. 应用与环境生物学报,2015,21(2):341-349
[21] 徐忠山,刘景辉,逯晓萍,等. 施用有机肥提高黑土土壤酶活性、增加细菌数量及种类多样性[J]. 中国土壤与肥料,2020,4(4):50-55
[22] YAN Y C,YAN R R,CHEN J Q,et al. Grazing modulates soil temperature and moisture in a Eurasian steppe[J]. Agricultural and Forest Meteorology,2018,262:157-165
[23] 商丽荣,万里强,李向林. 有机肥对羊草草原土壤细菌群落多样性的影响[J]. 中国农业科学,2020,53(13):2614-2624
[24] WANG Q F,JIANG X,GUAN D W,et al. Long-term fertilization changes bacterial diversity and bacterial communities in the maize rhizosphere of Chinese Mollisols[J]. Applied Soil Ecology,2017(125):88-96
[25] 张杰雪,王占青,全小龙,等. 高寒地区人工草地土壤微生物群落对不同种植方式和年限的响应[J]. 草地学报,2021,29(2):270-280
[26] 葛伟,董醇波,张芝元,等. 外生菌根真菌与内生细菌共生互作的研究进展[J].微生物学通报,2021,48(10):3810-3822
[27] 马龙,高伟,栾好安,等. 基于宏基因组学方法分析施肥模式对设施菜田土壤微生物群落的影响[J]. 植物营养与肥料学报,2021,27(3):403-416
[28] 翟盼盼. 不动杆菌全基因组测序鉴定分型及其致病相关性研究[D]. 衡阳:南华大学,2020:55-62
[29] 陈倩. 亚隔孢壳科的系统演化及分类学研究[D]. 北京:中国农业大学,2015:22-25
[30] 杨浩,金小琬,唐嘉淇,等. 裂殖壶菌合成二十二碳六烯酸机理研究进展[J]. 中国油脂,2019,44(8):109-115
[31] 李娥,胡华冉,李蛟男,等. 内生真菌提高植物抵御盐胁迫的研究进展[J]. 生物技术通报,2019,35(11):169-178
[32] 赵凤艳,张勇勇,张玥琦,等. 有机物料对设施番茄长期连作土壤细菌群落结构的影响[J]. 生态学杂志,2019,38(6):1732-1740
[33] 武发思. 长期定位施肥对黄土高原土壤微生物群落结构的影响[D]. 兰州:兰州大学,2009:53-58
[34] 王占青,张杰雪,杨雪莲,等. 高寒草甸不同斑块草地土壤微生物多样性特征研究[J]. 草地学报,2021,29(9):1916-1926

Effects of Applying Organic Fertilizer on Rhizosphere Microorganisms and Soil Physical and Chemical Properties of Elymus nutans

有机肥对垂穗披碱草根际微生物及土壤理化性质的影响

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	SONG Jian-chao, YANG Hang, JING Yuan-yuan, CHEN Yan-zhu, YU Xiao-jun. Effects of Exogenous GABA on Seed Germination and Physiological Characteristics of Elymus nutans under NaCl Stress [J]. Acta Agrestia Sinica, 2022, 30(2): 403-411.
[2]	DUAN Lian-xue, MA Xiang, JU Ze-liang, LIU Kai-qiang, HE Ji-tong, JIA Zhi-feng. Effects of Nitrogen Reduction Combined with Organic Fertilizer on Photosynthetic Characteristics and Yield of Avena sativa ‘Qinghai’ [J]. Acta Agrestia Sinica, 2022, 30(2): 471-478.
[3]	ZHANG Guang-ru, LUO Fang-lin, ZHANG Fa-wei, ZHU Jing-bin, HE Hui-dan, YANG Yong-sheng, WANG Chun-yu, LI Ying-nian. Vegetation and Soil Characteristics During Restoration of Grazing Rest in Warm Season of Degraded Alpine Meadows [J]. Acta Agrestia Sinica, 2022, 30(1): 144-152.
[4]	ZHANG Chu, WANG Miao, ZHANG Yu, WANG Xu, CHEN Jin-qiang, LI Rui-qiang, DAI Jing-zhong, BAI Yu-ting, XIN Xiao-ping, WEI Zhi-jun, CHENG Li, ZHAO Man, YAN Rui-rui. Effects of Root Cutting and Organic Fertilizer Application on Aboveground Biomass and Soil Nutrients in the Mowing Ggrassland of Leymus chinensis Meadow [J]. Acta Agrestia Sinica, 2022, 30(1): 220-228.
[5]	LIU Jing-jing, YIN Ya-li, LI Shi-xiong, ZHAO Wen, DONG Yi-ling, SU Shi-feng. Effects of Different Regulation Measures on Vegetation and Soil of Moderately Degraded Alpine Meadow [J]. Acta Agrestia Sinica, 2021, 29(9): 2074-2080.
[6]	QU Yan, SONG Qian, YANG He-long, ZHAO Kun, ZHAO Min, LIU Yu-ling, WANG De-ping, RONG Yu-ping. The Influence of Different Utilization Methods of Hulunbuir Grassland on the Structure of Soil Microbial Clusters [J]. Acta Agrestia Sinica, 2021, 29(8): 1621-1627.
[7]	GAO Zhi-xiang, LI Xi-lai, ZHANG Jing, JIN Li-qun, ZHOU Wei. Effects of Different Fertilization Treatments on Soil Enzyme Activities in Coal Mining Residuals of Alpine Mining Area [J]. Acta Agrestia Sinica, 2021, 29(8): 1748-1756.
[8]	SONG Jian-chao, YU Xiao-jun, WEI Kong-tao, WANG Peng-bin, TONG Yong-shang, LI Zhen, HE You-long, WANG Hai-bo. Effects of Nitrogen Application on Production Performance and Nutritional Quality of Elymus nutans in Alpine Region [J]. Acta Agrestia Sinica, 2021, 29(7): 1555-1564.
[9]	QIMEI La-mu, PUBU Zhuo-ma, CUI Tong-tong, ZHANG Xin-fei, JIANG Hui-na, FU Juan-juan, HU Tian-Ming. Effects of Exogenous Calcium on Physiology and Related Gene Expression of Elymus nutans under Cold Stress [J]. Acta Agrestia Sinica, 2021, 29(5): 919-928.
[10]	QIN Jin-ping, LIU Ying, MA Yu-shou, WANG Yan-long, LI Shi-xiong. Effects of Rest Grazing on Vegetation Characteristics and Photosynthetic Physiology of Dominant Species in Degraded Alpine Grasslands During Greenup Period [J]. Acta Agrestia Sinica, 2021, 29(5): 1034-1042.
[11]	YANG Yang, ZHANG Ni, JIANG Li-li, CHEN Ke-long. Effects of Simulated Precipitation on Soil Edaphic Physicochemical Factors and Microbial Community Characteristics in Bird Island of Qinghai Lake on The Tibetan Plateau [J]. Acta Agrestia Sinica, 2021, 29(5): 1043-1052.
[12]	BIAN Ying-ying, ZHANG Zhi-min, FU Zhen, LIU Wen-juan. Distribution Characteristics of Soil Microbial Communities of Different Vegetation Restoration Models and Their Correlation with Soil Physical and Chemical Properties in Desert Steppe [J]. Acta Agrestia Sinica, 2021, 29(4): 655-663.
[13]	SU Bei-bei, ZHANG Ying, DAO Ri-na. Study on the Distribution Characteristics of Bacterial Communities in the Rhizosphere Soil of Four Leguminous Cultivated Forages [J]. Acta Agrestia Sinica, 2021, 29(2): 250-258.
[14]	LI Wen, WEI Ting-hu, Abaodi, Yongcuobazhan, Cairentaci, ZHOU Yu-hai, LI Wen-hao, GUO Wei-xing, ZHANG Yan-ping. Effects of Reduction of Chemical Fertilizer and Organic Manure Supplement on Oat Nutrient Quality and Soil Nutrient in Alpine Cold Region [J]. Acta Agrestia Sinica, 2021, 29(12): 2878-2886.
[15]	YUE Li-nan, SHI Shang-li, QI Juan, YANG Pei-zhi, LIU Gang, LIU Wen-hui, ZHANG Ying-jun, JING Jing-ying. Effects of No-tillage Reseeding on Productivity and Nutritional Quality of Degraded Grassland in Northern China [J]. Acta Agrestia Sinica, 2021, 29(11): 2583-2590.