Abstract: In order to explore the effect of exogenous brassinosteroid priming on the antioxidant capacity of deteriorated seeds,Oat (Avena sativa) seeds were used as materials,and tested them with different concentrations (0,0.01,0.10,0.50,1.00,10.00 μmol·L^-1) brassinosteroid solution initiated for 0 (CK),1,6,12,18 and 24 h durations. The changes of germination rate,superoxide dismutase (SOD),catalase (CAT),ascorbate peroxidase (APX),glutathione reductase (GR) and malondialdehyde (MDA) content were analyzed. The results showed that low concentration (0.01~0.50 μmol·L^-1) of BR improved the germination rate of oat seeds,and increase the activities of SOD,CAT,APX and GR,and reduce the content of MDA in the oat seeds. When the concentration of BR reached 10.00 μmol·L^-1 (except for 18 h initiation),the germination rate,CAT,APX and GR activities were lower than those of the seeds in CK,and the MDA content was higher. Priming with 0.01 μmol·L^-1 brassinolide for 24 h may be the best treatment to alleviate the aging of oat seeds.

Key words: Brassinosteroids, Oats, Seed aging, Seed priming, Oxidation resistance

摘要： 为探讨外源油菜素内酯引发对劣变种子抗氧化能力的影响,试验采用燕麦(Avena sativa)种子为材料,人工老化后经不同浓度(0,0.01,0.10,0.50,1.00,10.00 μmol·L^-1)的油菜素内酯(Brassinosteroids,BR)溶液引发0(CK),1,6,12,18和24 h,分析了其发芽率、超氧化物歧化酶(Superoxide dismutase,SOD)、过氧化氢酶(Catalase,CAT)、抗坏血酸过氧化物酶(Ascorbate peroxidase,APX)、谷胱甘肽还原酶(glutathione reductase,GR)活性及丙二醛(Maloddialdehyde,MDA)含量的变化规律。结果表明:低浓度(0.01~0.50 μmol·L^-1)的BR可提高燕麦种子的发芽率,增加其SOD,CAT,APX和GR活性,降低其MDA含量;而BR浓度为10.00 μmol·L^-1时(除引发18 h外),其发芽率、CAT、APX和GR活性均低于CK,增加MDA含量。浓度为0.01 μmol·L^-1的油菜素内酯引发24 h可能是缓解燕麦种子老化的最佳处理。

关键词: 油菜素内酯, 燕麦, 种子老化, 种子引发, 抗氧化性能