Analysis on the Effect of Strain JB12 on Transcriptome for Flavonoids Synthesis of Chicory (Cichorium intybus) under Lead and Cadmium Stress

doi:10.11733/j.issn.1007-0435.2023.06.006

Acta Agrestia Sinica ›› 2023, Vol. 31 ›› Issue (6): 1648-1655.DOI: 10.11733/j.issn.1007-0435.2023.06.006

Analysis on the Effect of Strain JB12 on Transcriptome for Flavonoids Synthesis of Chicory (Cichorium intybus) under Lead and Cadmium Stress

JIN Zhong-min^1,2, LI Chun-yue¹, LIU Ben-song¹, LIU Bo¹, QI Xin¹, XIE Lin¹, LIU Li-jie^1,2, ZHANG Yan-fu^1,2, PAN Lin^1,2

1. College of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar, Heilongjiang Province 161006, China;
2. Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar University, Qiqihar, Heilongjiang Procince 161006, China

Received:2022-11-25 Revised:2023-03-05 Online:2023-06-15 Published:2023-06-30

菌株JB12影响铅镉胁迫下菊苣黄酮合成的转录组分析

金忠民^1,2, 李春月¹, 刘本松¹, 刘博¹, 齐欣¹, 解琳¹, 刘丽杰^1,2, 张艳馥^1,2, 潘林^1,2

1. 齐齐哈尔大学生命科学与农林学院, 黑龙江齐齐哈尔 161006;
2. 抗性基因工程与寒地生物多样性保护黑龙江省重点实验室, 黑龙江齐齐哈尔 161006

通讯作者: 金忠民,E-mail:yyy6768@163.com
作者简介:金忠民(1968-)，女，黑龙江绥化人，博士，教授，主要从事植物逆境生理与分子生物学研究，E-mail:yyy6768@163.com
基金资助:
黑龙江省省属高等学校基本科研业务费科研项目(No.135509131)资助

Abstract

Abstract: To investigate the effect of strain JB12 on the lead (Pb) and cadmium (Cd) resistance and flavonoid biosynthesis of chicory (Cichorium intybus) seedlings,strain JB12 was inoculated into soil with Pb-Cd concentrations of 400 and 40 mg·kg^-1. Seedling growth indicators,Pb and Cd content,antioxidant enzyme activity,total flavonoid content and enzyme activities related to the flavonoid biosynthesis were measured,and leaf transcriptome data were analyzed. The results showed that after inoculation with strain JB12,the levels of hydrogen peroxide (H₂O₂),malondialdehyde (MDA),Pb and Cd contents in the leaves and roots were significant lower than those of the only stressed group. The plant dry weight,antioxidant enzyme activity,total flavonoid content,and enzyme activities related to the flavonoid biosynthesis were higher than those of the only stressed group. The analysis of leaf transcriptome data and RT-qPCR validation showed that the up-regulation of phenylpropanoid and flavonoid biosynthesis pathway related genes expression might be an important mechanism for strain JB12 to improve the lead and cadmium resistance of chicory seedlings. In summary,the strain JB12 not only reduces the accumulation of Pb and Cd in chicory seedlings,but also enhances the activity of antioxidant enzymes and regulates the expression of genes related to flavonoid biosynthesis in leaves,therefore improving the Pb and Cd resistance and total flavonoid accumulation in chicory seedlings.

Key words: Pb-Cd stress, Burkholderia cepacian, Chicory, Transcriptome, Flavonoid biosynthesis

摘要： 为探究菌株JB12对菊苣(Cichorium intybus)幼苗铅镉抗性和黄酮生物合成的影响，将菌株JB12接种于铅镉复合(Pb-Cd)浓度为400~40 mg·kg^-1的土壤中，测定幼苗生长指标、铅镉含量、抗氧化酶活性、总黄酮含量和黄酮生物合成相关酶活性，并对叶片转录组数据进行分析。结果表明，接种菌株JB12后，叶片过氧化氢(H₂O₂)、丙二醛(Malondialdehyde，MDA)及地上部和根部铅镉含量均低于胁迫处理组，植株干重、抗氧化酶活性、总黄酮含量及黄酮生物合成相关酶活性均高于胁迫处理组。对叶片转录组数据和RT-qPCR验证分析表明，苯丙素和黄酮生物合成途径相关基因表达上调可能是菌株JB12提高菊苣幼苗铅镉抗性的重要机制。综上所述，菌株JB12在减少菊苣幼苗铅镉积累的同时，还可以通过增强抗氧化酶活性及调控叶片黄酮生物合成相关基因的表达，提高菊苣幼苗的铅镉抗性和总黄酮的积累。

关键词: 铅镉胁迫, 洋葱伯克霍尔德氏菌, 菊苣, 转录组, 黄酮生物合成

CLC Number:

S476

JIN Zhong-min, LI Chun-yue, LIU Ben-song, LIU Bo, QI Xin, XIE Lin, LIU Li-jie, ZHANG Yan-fu, PAN Lin. Analysis on the Effect of Strain JB12 on Transcriptome for Flavonoids Synthesis of Chicory (Cichorium intybus) under Lead and Cadmium Stress[J]. Acta Agrestia Sinica, 2023, 31(6): 1648-1655.

金忠民, 李春月, 刘本松, 刘博, 齐欣, 解琳, 刘丽杰, 张艳馥, 潘林. 菌株JB12影响铅镉胁迫下菊苣黄酮合成的转录组分析[J]. 草地学报, 2023, 31(6): 1648-1655.

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Analysis on the Effect of Strain JB12 on Transcriptome for Flavonoids Synthesis of Chicory (Cichorium intybus) under Lead and Cadmium Stress

菌株JB12影响铅镉胁迫下菊苣黄酮合成的转录组分析

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