Acta Agrestia Sinica ›› 2024, Vol. 32 ›› Issue (5): 1370-1377.DOI: 10.11733/j.issn.1007-0435.2024.05.007

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Cloning and Expression Analysis of MsJAR1 in Medicago sativa

DAI Rui1, CHEN Qi1, SHUANG Shuang1, ZHANG Yan1, ZHANG Zhi-qiang1,2, MI Fu-gui1,2   

  1. 1. Colleg of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Inner Mongolia Autonomous Region, Hohhot, 010010, China;
    2. Technology Engineering Center of Drought and Cold-resistant Grass Breeding in North of the National Forestry and Grassland Administration, Institute of Grassland and Resources Environment, Inner Mongolia Agricultural University, Hohhot, Inner Mongalia 010010, China
  • Received:2023-12-06 Revised:2024-01-18 Published:2024-06-01

紫花苜蓿MsJAR1基因克隆及表达分析

代蕊1, 陈崎1, 爽爽1, 张岩1, 张志强1,2, 米福贵1,2   

  1. 1. 内蒙古农业大学草原与资源环境学院, 内蒙古 呼和浩特 010010;
    2. 内蒙古农业大学草原与资源环境学院, 国家林业草原北方抗旱耐寒草品种育繁工程技术研究中心, 内蒙古 呼和浩特 010010
  • 通讯作者: 张志强,E-mail:zhangzq19890102@126.com;米福贵,E-mail:Mfgui@yahoo.com.cn
  • 作者简介:代蕊(1999-),女,蒙古族,内蒙古赤峰人,硕士研究生,主要从事草育种方面研究,E-mail:18747659997@163.com
  • 基金资助:
    内蒙古自治区高等学校“青年科技英才支持项目”(NJYT23009);国家自然科学基金(32160333;32060388);苜蓿分子育种体系构建及种质创制(BR22-11-12);苜蓿新品种选育及制繁种关键技术研发(CCPTZX2023B04);内蒙古自治区研究生科研创新项目(S202311029)资助

Abstract: To explore the function of jasmonoyl amino acid conjugate synthase(JAR1) gene in alfalfa,a novel gene, MsJAR1 (OK602801),was cloned,characterized and bioinformatic analyzed from Medicago sativa L.. The results showed that the cDNA sequence of MsJAR1 gene was 1 740 bp in length,and the open reading frame encoded 579 amino acid polypeptide,containing the GH3 conserved domain. Amino acid sequence alignment indicated that the homology of deduced MsJAR1 with Medicago truncatula,Trifolium pratense,Pisum sativum,Cicer arietinum and Abrus precatorius was all more than 80%. Subcellular localization analysis in tobacco showed that the MsJAR1 protein located in the chloroplast. The MsJAR1 gene expression was the highest in old leaves,followed by floral organs. In addition,thrips feeding,salt and drought stress could induce the expression of MsJAR1 gene,which provided a reference for further exploring the function of MsJAR1 gene in alfalfa.

Key words: Alfalfa, MsJAR1, Gene cloning, Expression analysis

摘要: 为探究紫花苜蓿(Medicago sativa L.)茉莉酰氨基酸结合物合成酶(Jasmonoyl amino acid conjugate synthase,JAR1)的基因功能,本试验对'草原4号’紫花苜蓿中克隆到的MsJAR1基因进行了生物信息学和表达模式分析,发现MsJAR1基因cDNA序列长度为1 740 bp,开放阅读框(ORF)编码 579 个氨基酸残基,含有GH3保守结构域。MsJAR1与蒺藜苜蓿(Medicago truncatula)、红三叶(Trifolium pratense)、豌豆(Pisum sativum)、鹰嘴豆(Cicer arietinum)、相思子(Abrus precatorius)的同源性均在80%以上。烟草亚细胞定位显示该蛋白定位于叶绿体。MsJAR1基因在老叶中表达量最高,花器官次之;蓟马取食、盐和干旱胁迫可诱导该基因表达,初步推测其参与紫花苜蓿应答蓟马取食及干旱和盐胁迫防御反应。本研究为进一步探究紫花苜蓿MsJAR1基因功能提供了参考。

关键词: 紫花苜蓿, MsJAR1, 基因克隆, 表达模式

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