Effects of Mixed Saline-Alkali Stress on Physiology and Gene Expression of Alfalfa Seedlings

doi:10.11733/j.issn.1007-0435.2024.04.007

Acta Agrestia Sinica ›› 2024, Vol. 32 ›› Issue (4): 1044-1054.DOI: 10.11733/j.issn.1007-0435.2024.04.007

Effects of Mixed Saline-Alkali Stress on Physiology and Gene Expression of Alfalfa Seedlings

ZHU Chen-chen¹, SHI Kun¹, HE Qin-kun¹, ZHENG Yu-qin¹, YUAN Feng², LIU Ya-ling², WANG Zan¹

1. College of Grassland Science and Technology, China Agricultural University, Beijing 100193, China;
2. Inner Mongolia Pratacultural Technology Innovation Center Co., Ltd, Hohhot, Inner Mongolia 010070, China

Received:2024-01-12 Revised:2024-03-20 Published:2024-05-07

混合盐碱胁迫对紫花苜蓿幼苗生理和基因表达的影响

朱晨晨¹, 史昆¹, 何沁坤¹, 郑雨琴¹, 苑峰², 刘亚玲², 王赞¹

1. 中国农业大学草业科学与技术学院, 北京 100193;
2. 内蒙古草业技术创新中心有限公司, 内蒙古呼和浩特 010070

通讯作者: 王赞,E-mail:zanwang@cau.edu.cn
作者简介:朱晨晨(1996-),女，汉族，山东聊城人，博士研究生，主要从事牧草基因资源挖掘与利用的相关研究，E-mail:zhucc@cau.edu.cn
基金资助:
国家草业技术创新中心(筹)重大创新平台建设专项(CCPTZX2023B04)；国家林业和草原种质资源库(2005DKA21003)资助

Abstract

Abstract: In this study,two alfalfa (Medicago sativa L.) materials 'Baoding’ and 'ZN-1’ were treated with the mixed saline-alkali solution (NaCl∶Na₂SO₄∶NaHCO₃∶Na₂CO₃ in 1∶1∶1∶1 ratio at pH 9.3) at the concentrations of 0 mmol·L^-1,40 mmol·L^-1 and 80 mmol·L^-1,and compared for the tolerance. The morphology,physiology,and expression of the selected genes that are involved in ROS-scavenging,ion transport,and photosynthetic regulation were investigated at the alfalfa seedling stage,to look into the response to mixed saline-alkali stress. The results showed that with the increase of mixed saline-alkali concentration,significant (P＜0.05) damage was observed in the plant height,dry weight,chlorophyll content,photosynthetic rate,and Fv/Fm,in both the alfalfa materials. Compared with 'Baoding’,'ZN-1’ demonstrated significantly higher saline-alkali tolerance,as evidenced by higher plant height,more biomass,more contents of chlorophyll content,proline,and soluble sugar,stronger antioxidant activity,promoted robust photosynthesis,and less accumulation of ROS in leaves and Na⁺in root. In 'ZN-1’,the significantly up-regulated genes were associated with the pathways of ROS scavenging,proline biosynthesis,Na⁺ vacuolar compartmentalization,and chlorophyll maintenance. In summary,proline biosynthesis,Na⁺ vacuolar compartmentalization,chlorophyll maintenance,and antioxidants may play an important role in the 'ZN-1’ tolerance of mixed saline-alkali stress.

Key words: Alfalfa, Mixed saline-alkali stress, Physiological responses, Genes expression

摘要： 本研究以'ZN-1’和'保定’苜蓿(Medicago sativa L.'Baoding’)为试验材料，以浓度梯度为0 mmol·L^-1，40 mmol·L^-1和80 mmol·L^-1，pH值为9.3的混合盐碱溶液(NaCl∶Na₂SO₄∶NaHCO₃∶Na₂CO₃摩尔比为1∶1∶1∶1)进行胁迫处理，比较2个紫花苜蓿幼苗的生长指标、生理指标和基因表达(ROS清除、离子转运和光合调控3类基因)差异,以期为解析紫花苜蓿耐混合盐碱胁迫响应机制提供理论依据。结果表明，随着混合盐碱胁迫浓度的增加，2个紫花苜蓿的株高、干重、叶绿素含量、光合速率以及Fv/Fm等均出现明显的损伤(P＜0.05)。其中，'ZN-1’的耐混合盐碱性显著高于'保定’苜蓿，主要表现为'ZN-1’的株高和生物量更高、叶绿素含量和抗氧化酶的活性更高，净光合速率更强，脯氨酸和可溶性糖含量更高，叶片ROS积累量和地下部Na⁺积累更少，ROS清除、脯氨酸生物合成、Na⁺液泡区隔化以及叶绿素维持相关编码基因的表达水平更高。因此，脯氨酸生物合成、Na⁺液泡区隔化、叶绿素维持以及抗氧化酶可能在'ZN-1’耐混合盐碱胁迫中发挥重要作用。

关键词: 紫花苜蓿, 混合盐碱胁迫, 生理响应, 基因表达

CLC Number:

S541.9

ZHU Chen-chen, SHI Kun, HE Qin-kun, ZHENG Yu-qin, YUAN Feng, LIU Ya-ling, WANG Zan. Effects of Mixed Saline-Alkali Stress on Physiology and Gene Expression of Alfalfa Seedlings[J]. Acta Agrestia Sinica, 2024, 32(4): 1044-1054.

朱晨晨, 史昆, 何沁坤, 郑雨琴, 苑峰, 刘亚玲, 王赞. 混合盐碱胁迫对紫花苜蓿幼苗生理和基因表达的影响[J]. 草地学报, 2024, 32(4): 1044-1054.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2024.04.007

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2024/V32/I4/1044

References

[1] WANG H,TAKANO T,LIU S K. Screening and evaluation of saline-alkaline tolerant germplasm of rice (Oryza sativa L.) in soda saline-alkali soil[J]. Agronomy Basel,2018,8(10):205
[2] PARK H J,KIM W Y,YUN D J. A new insight of salt stress signaling in plant[J]. Molecules and Cells,2016,39(6):447-459
[3] RENGASAMY P. World salinization with emphasis on Australia[J]. Journal of Experimental Botany,2006,57(5):1017-1023
[4] YANG J,YAO S,WANG X,et al. Halt soil salinization,boost soil productivity[J]. Science,2021,73:30-34
[5] KAIWEN G,ZISONG X,YUZE H,et al. Effects of salt concentration,pH,and their interaction on plant growth,nutrient uptake,and photochemistry of alfalfa (Medicago sativa) leaves[J]. Plant Signaling & Behavior,2020,15(12):1832373
[6] MANSOUR M M F,HASSAN F A S. How salt stress-responsive proteins regulate plant adaptation to saline conditions[J]. Plant Molecular Biology,2022,108(3):175-224
[7] FENG N,YU M,LI Y,et al. Prohexadione-calcium alleviates saline-alkali stress in soybean seedlings by improving the photosynthesis and up-regulating antioxidant defense[J]. Ecotoxicology and Environmental Safety,2021,220:112369
[8] WANG J,ZHANG Y,YAN X,et al. Physiological and transcriptomic analyses of yellow horn (Xanthoceras sorbifolia) provide important insights into salt and saline-alkali stress tolerance[J]. Plos One,2020,15(12):e0244365
[9] ZHANG H,XU Z,GUO K,et al. Toxic effects of heavy metal Cd and Zn on chlorophyll,carotenoid metabolism and photosynthetic function in tobacco leaves revealed by physiological and proteomics analysis[J]. Ecotoxicology and Environmental Safety,2020,202:110856
[10] WANG X S,REN H L,WEI Z W,et al. Effects of neutral salt and alkali on ion distributions in the roots,shoots,and leaves of two alfalfa cultivars with differing degrees of salt tolerance[J]. Journal of Integrative Agriculture,2017,16(8):1800-1807
[11] CHEN M,YANG Z,LIU J,et al. Adaptation mechanism of salt excluders under saline conditions and its applications[J]. International Journal of Molecular Sciences,2018,19(11):3668
[12] VERMA D,JALMI S K,BHAGAT P K,et al. A bHLH transcription factor,MYC2,imparts salt intolerance by regulating proline biosynthesis in Arabidopsis[J]. FEBS Journal,2020,287(12):2560-2576
[13] LIU L,LIU D,WANG Z,et al. Exogenous allantoin improves the salt tolerance of sugar beet by increasing putrescine metabolism and antioxidant activities[J]. Plant Physiology and Biochemistry,2020,154:699-713
[14] ZHANG L,SUN Y,JI J,et al. Flavonol synthase gene MsFLS13 regulates saline-alkali stress tolerance in alfalfa[J]. The Crop Journal,2023,11(4):1218-1229
[15] LEI Y G,HANNOUFA A,YU PQ. The use of gene modification and advanced molecular structure analyses towards improving alfalfa forage[J]. International Journal of Molecular Sciences,2017,18(2):298
[16] SINGER S D,HANNOUFA A,ACHARYA S. Molecular improvement of alfalfa for enhanced productivity and adaptability in a changing environment[J]. Plant Cell and Environment,2018,41(9):1955-1971
[17] 刁婵,王文信. 中国苜蓿草进口贸易格局及其影响因素[J]. 草业科学,2023,40(9):2424-2434
[18] 贾壮壮,谭亚男,管孝艳,等. 宁夏盐碱地成因及分区治理措施综述[J]. 灌溉排水学报,2023,42(5):122-134
[19] LEI Y,XU Y,HETTENHAUSEN C,et al. Comparative analysis of alfalfa (Medicago sativa L.) leaf transcriptomes reveals genotype-specific salt tolerance mechanisms[J]. BMC Plant Biology,2018,18(1):35
[20] MA L,LI X,ZHANG J,et al. MsWRKY33 increases alfalfa (Medicago sativa L.) salt stress tolerance through altering the ROS scavenger via activating MsERF5 transcription[J]. Plant,Cell & Environment,2023,46(12):3887-3901
[21] SONG T,XU H,SUN N,et al. Metabolomic analysis of Alfalfa (Medicago sativa L.) root-symbiotic rhizobia responses under alkali stress[J]. Frontiers in Plant Science,2017,8:1208
[22] YU J,YUAN Y,ZHANG W,et al. Overexpression of an NF-YC2 gene confers alkali tolerance to transgenic alfalfa (Medicago sativa L.)[J]. Frontiers in Plant Science,2022,13:960160
[23] 王晓春,杨天辉,王川,等. 混合盐碱胁迫对紫花苜蓿的生长和生理指标的影响[J]. 中国农学通报,2022,38(19):139-145
[24] 张晓磊,刘晓静,齐敏兴,等. 混合盐碱对紫花苜蓿苗期根系特征的影响[J]. 中国生态农业学报,2013,21(3):340-346
[25] WANG Y,WANG J,GUO D,et al. Physiological and comparative transcriptome analysis of leaf response and physiological adaption to saline alkali stress across pH values in alfalfa (Medicago sativa)[J]. Plant Physiology and Biochemistry,2021,167:140-152
[26] 赵海明,游永亮,李源,等. 紫花苜蓿资源抗旱性鉴定评价方法研究[J]. 草地学报,2017,25(6):1308-1316
[27] 景芳,师尚礼,南攀等.不同苜蓿品种叶片特征、光合生理特性与产量性状的比较[J].草地学报,2024,32(2):369-377
[28] 武祎,田雨,宋彦涛. 不同盐分对黄花苜蓿早期幼苗生长及离子积累的影响[J]. 中国草地学报,2019,41(4):39-44
[29] 王雪萌,何欣,张涵,等. 基于多光谱成像技术快速无损检测紫花苜蓿人工老化种子[J]. 草业学报,2022,31(7):197-208
[30] FRYER M J,OXBOROUGH K,MULLINEAUX P M,et al. Imaging of photo-oxidative stress responses in leaves[J]. Journal of Experimental Botany,2002,53(372):1249-1254
[31] GIANNOPOLITIS C N,RIES S K. Superoxide dismutases:I. Occurrence in higher plants[J]. Plant Physiology,1977,59(2):309-314
[32] PANDOLFINI T,GABBRIELLI R,COMPARINI C. Nickel toxicity and peroxidase-activity in seedlings of Triticum-aestivum L[J]. Plant Cell and Environment,1992,15(6):719-725
[33] 梁欢,韦宝,陈静,等. 基于叶绿素荧光参数的紫花苜蓿种质苗期抗旱性评价[J]. 草地学报,2020,28(1):45-55
[34] KRAMER D M,JOHNSON G,KIIRATS O,et al. New fluorescence parameters for the determination of Q_A redox state and excitation energy fluxes[J]. Photosynthesis Research,2004,79(2):209-218
[35] LING L,AN Y,WANG D,et al. Proteomic analysis reveals responsive mechanisms for saline-alkali stress in alfalfa[J]. Plant Physiology and Biochemistry,2022,170:146-159
[36] LACOUR T,BABIN M,LAVAUD J. Diversity in xanthophyll cycle pigments content and related nonphotochemical quenching (NPQ) among microalgae:implications for growth strategy and ecology[J]. Journal of Phycology,2020,56(2):245-263
[37] DU B,ZHAO W,AN Y,et al. Overexpression of an alfalfa glutathione S-transferase gene improved the saline-alkali tolerance of transgenic tobacco[J]. Biology Open,2019,8(9):bio043505
[38] KUMAR S,PANDEY A K. Chemistry and biological activities of flavonoids:an overview[J]. The Scientific World Journal,2013,2013:162750
[39] DANGRIT D,SOMPORNPAILIN K. Antioxidant activities of transgenic flower over-expressing FLS and TT8 involving in flavonoid biosynthesis[J]. Applied Mechanics and Materials,2018,879:78-82
[40] STRACKE R,ISHIHARA H,HUEP G,et al. Differential regulation of closely related R2R3-MYB transcription factors controls flavonol accumulation in different parts of the Arabidopsis thaliana seedling[J]. The Plant Journal,2007,50(4):660-677
[41] RAMENENI J J,DHANDAPANI V,PAUL P,et al. F-Box genes in Brassica rapa:genome-wide identification,structural characterization,expressional validation,and comparative analysis[J]. Plant Molecular Biology Reporter,2018,36(3):500-517
[42] STEINHORST L,KUDLA J. How plants perceive salt[J]. Nature,2019,572(7769):318-320
[43] MANSOUR M M F,EMAM M M,SALAMA K H A,et al. Sorghum under saline conditions:responses,tolerance mechanisms,and management strategies[J]. Planta,2021,254(2):24
[44] LIU C,YOUNG A L,STARLING WINDHOF A,et al. Coupled chaperone action in folding and assembly of hexadecameric Rubisco[J]. Nature,2010,463(7278):197-202
[45] ZHOU C,HAN L,PISLARIU C,et al. From model to crop:functional analysis of a STAY-GREEN Gene in the model legume Medicago truncatula and effective use of the gene for alfalfa improvement[J]. Plant Physiology,2011,157(3):1483-1496
[46] SAKURABA Y,PARK S Y,KIM Y S,et al. Arabidopsis STAY-GREEN2 is a negative regulator of chlorophyll degradation during leaf senescence[J]. Molecular Plant,2014,7(8):1288-1302
[47] GUTTERIDGE S,GATENBY A A. Rubisco synthesis,assembly,mechanism,and regulation[J]. The Plant Cell,1995,7(7):809-819
[48] 刘晶,才华,刘莹,等. 两种紫花苜蓿苗期耐盐生理特性的初步研究及其耐盐性比较[J]. 草业学报,2013,22(2):250-256
[49] 于明倩,胡亚娜,田雨. 混合盐碱胁迫对紫花苜蓿幼苗生长和生理特性的影响[J]. 黑龙江畜牧兽医,2021,(22):109-112
[50] FANG S,HOU X,LIANG X. Response mechanisms of plants under saline-alkali stress[J]. Frontiers in Plant Science,2021,12:667458
[51] 林霞,郑坚,陈秋夏,等. NaCl胁迫对无柄小叶榕光合作用和抗氧化酶活性的影响[J]. 北京林业大学学报,2011,33(4):70-74
[52] GILL S S,TUTEJA N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants[J]. Plant Physiology and Biochemistry 2010,48(12):909-930
[53] ALI S,TYAGI A,BAE H. ROS interplay between plant growth and stress biology:challenges and future perspectives[J]. Plant Physiology and Biochemistry,2023,203:108032
[54] ZHANG H,LIU X L,ZHANG R X,et al. Root damage under alkaline stress is associated with reactive oxygen species accumulation in rice (Oryza sativa L.)[J]. Frontiers in Plant Science,2017,8:1580

Effects of Mixed Saline-Alkali Stress on Physiology and Gene Expression of Alfalfa Seedlings

混合盐碱胁迫对紫花苜蓿幼苗生理和基因表达的影响

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	CHEN Qi, DAI Rui, ZHANG Yan, SHUANG Shuang, HUO Xiao-wei, BIAN Lin, GUO Na, ZHANG Zhi-qiang, ZHANG Yu-tong. Genes Expression Analysis of Flavonoid Biosynthesis Induced by Thrips Feeding in Alfalfa [J]. Acta Agrestia Sinica, 2024, 32(4): 1034-1043.
[2]	WANG Jie, LIU MEI-jun, ZHAO Yao-yao, ZHANG Zheng, WEI Dan-dan, GONG Liang-zhu, HU Xuan-ming. Effects of Betaine on Respiratory Electron Transport of Alfalfa Seeds During Germination Under Low Temperature [J]. Acta Agrestia Sinica, 2024, 32(3): 763-771.
[3]	JING Fang, SHI Shang-li, NAN Pan, MA Rui-hong, A Yun, LU Bao-fu, GUAN Jian, ZHANG Hui-hui. Comparison of Leaf Characteristics, Photosynthetic Physiological Characteristics, and Yield Traits of Different Alfalfa Varieties [J]. Acta Agrestia Sinica, 2024, 32(2): 369-377.
[4]	LIU Jing-yu, ZHAO Hai-jun, OU Cheng-ming, JIA Zhi-cheng, MAO Pei-sheng. Effects of Melatonin Priming on Alfalfa Seed Germination Characteristics Under Na₂SO₄ Stress [J]. Acta Agrestia Sinica, 2024, 32(2): 378-385.
[5]	LI Qi-jiao, CUI Dong-yi, DUAN Xin-hui, LIU Li, XU Wen-hua, LI Qing-xi, ZHENG Li-wen, REN Jian, ZHU Li-min, MA Xiang-li. Effects of Melatonin on Seed Germination and Antioxidant Physiology of Alfalfa Seedlings Under Aluminum Stress [J]. Acta Agrestia Sinica, 2024, 32(2): 535-542.
[6]	GONG Ze-lin, EER Demutu, SU Xiao-gang, CHENG Rong-rong, YANG He-ming, ZHANG Xiao-lin, ZHAI Peng-hui. Effects of Water and Fertilizer Addition on Soil Respiration Rate in Grass-field Rotation System [J]. Acta Agrestia Sinica, 2024, 32(2): 562-569.
[7]	LI Xiao-hong, WANG Xiao-tong, MA Xu-xia, CAI Wen-qi, FENG Xue-li, MA Meng-fan, LI Shu-xia. Identification and Analysis of CNGC Gene Family Members in Alfalfa [J]. Acta Agrestia Sinica, 2024, 32(2): 588-598.
[8]	WU Qian-qian, SU Ya, ZENG Xiang-ming, LI Dong-heng, CHENG Wei, HAO Jun. Extraction Method Screening and Process Optimization of Alfalfa and White Clover Selenoprotein [J]. Acta Agrestia Sinica, 2024, 32(2): 654-660.
[9]	LI Xin, WU Jing-ye, CHEN Fei-er, FAN Lu, MA Lin, WANG Xue-min. Effects of Transcriptional Regulation of MsACS2 by MsWRKY33 on Salt Tolerance of Alfalfa [J]. Acta Agrestia Sinica, 2024, 32(1): 54-65.
[10]	ZHANG Ling, MA Dong-mei, LIU Xiao-xia, MA Qiao-li, HOU Wen-jun, LI Jia-wen, SU Li-na, HANG Jia-hui. The Effects of Exogenous Melatonin on Seedling Physiological Characteristics of Alfalfa under Drought Stress [J]. Acta Agrestia Sinica, 2024, 32(1): 198-206.
[11]	ZHU Min, NIU Shuai-shuai, HOU Qing-qing, YANG Xuan, XU Hong-yu. The Response of Alfalfa Production in Jinzhong Basin, Shanxi Province to Water and Air Temperature [J]. Acta Agrestia Sinica, 2024, 32(1): 207-218.
[12]	CHEN Hai-yan, HUANG Rong, ZHAO Liang, YANG Jie, XIE Jin-jing, ZHANG Zhen-fen. Effects of Cp2-EPS on the Morphology and Photosynthesis Characteristics of Alfalfa Seedlings under Salt Stress [J]. Acta Agrestia Sinica, 2024, 32(1): 229-238.
[13]	LI Qing-pu, BAI Jian-hai, YAO Tuo, LEI Yang, ZHOU Ze, ZHANG Chen, FU Wei-gang. Effects of the Combined Application of Microbial Inoculant and Nitrogen Fertilizer Reduction on the Growth, and Soil Physicochemical Properties of Alfalfa in Hexi Area [J]. Acta Agrestia Sinica, 2024, 32(1): 314-321.
[14]	WU Ling-ling, XIE Yong-li, YANG Jie, YANG Xue, LI Jun-xi, WANG Tian, GAO Ying, CHANG Fei-fei. Biological Activity of Bacillus WL520 and Its Growth-promoting Effect on Alfalfa under Drought Stress [J]. Acta Agrestia Sinica, 2024, 32(1): 322-330.
[15]	LI Le, LI Dan-ning, YANG Ye-yan, PAN Xin-ya, WAN Yi-qi, ZHANG Guo-yun, XI Jie-jun, WANG Ya-fang, YANG Pei-zhi. Analysis of Metabolomics and Screening of Stomatal Regulating Substances in Alfalfa Leaves under Drought Stress [J]. Acta Agrestia Sinica, 2023, 31(9): 2671-2683.