Abstract: The effects of shading treatment on differentially expressed genes in leaves of Aconitum kusnezoffii were analyzed to reveal the molecular mechanism of its responses to shading. Through the transcriptome sequencing of the leaves of A. kusnezoffii after shading treatment,it was found that the data quality was high (Q30 ≥ 94.37 %),and 59 082 Unigenes (N50 = 1 826 bp) were obtained by splicing and assembly. Functional annotation showed that 27 668 Unigenes (46.83 %) were annotated to multiple important databases. Analysis of differentially expressed genes found 483 differentially expressed genes,of which 421 were annotated. It was found that light conditions significantly affected the biological processes such as photosynthesis,hormone regulation and energy metabolism of A. kusnezoffii. Key gene analysis showed that LHCB17,ATPβ and PetA were down-regulated under shading,LHCB1 and GA2ox were up-regulated. The regulation of these genes revealed the effect of light on the growth and metabolic adaptability of A. kusnezoffii. Real-time fluorescence quantitative analysis verified the reliability of transcriptome data.The results of this study not only enhance the understanding of the adaptation of A. kusnezoffii to low light environment,but also provide clues for the study of its genetic regulatory network and functional genes,which is of great significance for the rational cultivation and utilization of A. kusnezoffii.

Key words: Aconitum kusenezoffii L., Transcriptome, Shade, qRT-PCR

摘要： 研究通过分析遮荫处理对草乌头(Aconitum kusnezoffii L.)叶片中差异表达基因的影响，揭示其响应遮荫的分子机制。通过对遮荫处理后草乌头叶片的转录组测序，发现数据质量较高(Q30≥94.37%)，并通过拼接和组装得到59 082个Unigenes(N50为1 826 bp)。功能注释表明，有27 668个Unigenes(46.83%)被注释到多个重要数据库。差异表达基因分析筛选出483个差异基因，其中421个获得注释。研究发现，光照条件显著影响草乌头的光合作用、激素调控和能量代谢等生物过程。关键基因分析显示，LHCB17，ATPβ，PetA等在遮荫下表达下调，LHCB1，GA2ox等表达上调。这些基因的调控作用揭示了光照对草乌头生长和代谢适应性的影响。实时荧光定量分析验证了转录组数据的可靠性。研究结果不仅增进了对草乌头适应光照环境的理解，还为其遗传调控网络和功能基因的研究提供了线索，对草乌头的合理栽培和利用具有重要意义。

关键词: 草乌头, 转录组, 遮荫, qRT-PCR