Identification of Alfalfa NAC Gene Family and Analysis of Their Expression Patterns under Abiotic Stress

doi:10.11733/j.issn.1007-0435.2024.08.011

Acta Agrestia Sinica ›› 2024, Vol. 32 ›› Issue (8): 2440-2458.DOI: 10.11733/j.issn.1007-0435.2024.08.011

Identification of Alfalfa NAC Gene Family and Analysis of Their Expression Patterns under Abiotic Stress

ZOU Wei-peng, ZHAI Jia-xing, LI Di-na, HUANG Jie-qiong, GUO Kang-jie, CEN Hui-fang, ZHU Hui-sen, XU Tao

College of Grassland Science, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China

Received:2024-04-01 Revised:2024-05-17 Published:2024-09-07

紫花苜蓿NAC基因家族鉴定及在非生物胁迫下的表达模式分析

邹苇鹏, 翟佳兴, 李迪娜, 黄洁琼, 郭康杰, 岑慧芳, 朱慧森, 许涛

山西农业大学草业学院, 山西太谷 030801

通讯作者: 岑慧芳,E-mail:cenhuifangqk@163.com;朱慧森,E-mail:zhuhuisen@126.com
作者简介:邹苇鹏(1999-),男,汉族,四川德阳人,硕士研究生,主要从事草类种质资源研究,E-mail:2015389003@qq.com
基金资助:
山西省基础研究计划青年科学基金项目(20210302124607);国家自然科学基金(32071872，31402131);山西省中央引导地方科技发展资金项目(YDZJSX2022B006);山西重点研发计划课题(202102140601006-3);山西农业大学博士科研启动专项(2021BQ01)资助

Abstract

Abstract: The NAC transcription factors are specific to plants and play a crucial role in regulating plant growth,development,and response to abiotic stresses. A total of 143 NAC family transcription factors were identified in the ‘Zhongmu No.4’ alfalfa (Medicago sativa) genome using bioinformatics methods,specifically in response to abiotic stress such as drought. The distribution of MsNAC genes on the chromosomes of alfalfa was found to be uneven,with fragment repetition and collinear relationships with soybean (Glycine max) and Medicago truncatula. Gene conserved motifs and gene structure analysis revealed that most MsNAC genes contain Motif1 and Motif3 conserved motifs and 3~6 exons,most MsNAC proteins are localized in the nucleus. The results of phylogenetic tree analysis showed that MsNACs were clustered into 8 subfamilies. The analysis of cis-acting elements showed that MsNAC genes contain cis-acting elements to drought,abscisic acid and auxin. Fluorescence quantitative PCR analysis showed that some MsNAC genes were responsive to drought,salt stress,and ABA treatment,most MsNAC candidate genes were up-regulated after drought,salt and ABA treatment. These findings provide valuable candidate genes for breeding alfalfa with improved drought and salt tolerance.

Key words: Alfalfa, NAC, Transcription factor, Bioinformatics, Abiotic stress, Expression pattern

摘要： NAC是植物特有转录因子，在调控植物生长发育及响应非生物胁迫等方面发挥作用。为挖掘紫花苜蓿(Medicago sativa)响应干旱等非生物胁迫的NAC转录因子，本研究利用生物信息学方法在‘中苜4号’紫花苜蓿基因组中鉴定出143个NAC家族转录因子。研究表明，紫花苜蓿MsNAC基因在染色体上不均匀分布，MsNAC基因存在片段重复，并与大豆(Glycine max (Linn.) Merr.)、蒺藜苜蓿(Medicago truncatula)存在共线性关系，基因保守基序及基因结构分析表明MsNAC基因多数含有Motif1，Motif3保守基序及3~6个外显子。大多数MsNAC蛋白定位于细胞核中。系统进化树分析结果表明MsNACs聚类为8个亚家族。顺式作用元件分析表明MsNAC基因含有响应干旱及脱落酸、生长素等顺式作用元件。荧光定量分析结果显示部分MsNAC基因响应干旱、盐胁迫及脱落酸处理，大部分MsNAC候选基因在干旱、盐及ABA处理后上调表达。本研究结果可为紫花苜蓿抗旱、耐盐分子育种提供候选基因。

关键词: 紫花苜蓿, NAC, 转录因子, 生物信息学, 非生物胁迫, 表达模式

CLC Number:

S514.9

ZOU Wei-peng, ZHAI Jia-xing, LI Di-na, HUANG Jie-qiong, GUO Kang-jie, CEN Hui-fang, ZHU Hui-sen, XU Tao. Identification of Alfalfa NAC Gene Family and Analysis of Their Expression Patterns under Abiotic Stress[J]. Acta Agrestia Sinica, 2024, 32(8): 2440-2458.

邹苇鹏, 翟佳兴, 李迪娜, 黄洁琼, 郭康杰, 岑慧芳, 朱慧森, 许涛. 紫花苜蓿NAC基因家族鉴定及在非生物胁迫下的表达模式分析[J]. 草地学报, 2024, 32(8): 2440-2458.

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Identification of Alfalfa NAC Gene Family and Analysis of Their Expression Patterns under Abiotic Stress

紫花苜蓿NAC基因家族鉴定及在非生物胁迫下的表达模式分析

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