The Response of Potential N2O Emission from an Alpine Meadow Soil to Simulated Livestock Grazing

doi:10.11733/j.issn.1007-0435.2024.09.013

Acta Agrestia Sinica ›› 2024, Vol. 32 ›› Issue (9): 2794-2802.DOI: 10.11733/j.issn.1007-0435.2024.09.013

The Response of Potential N₂O Emission from an Alpine Meadow Soil to Simulated Livestock Grazing

WU Xiao-fen^1,2, CHEN You-chao^1,2, JIANG Wen-ting^1,2, SHEN Yu-ye^1,2, CAI Yan-jiang^1,2

1. State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, Zhejiang Province 311300, China;
2. College of Environment and Resources/College of Carbon Neutrality, Zhejiang A&F University, Hangzhou, Zhejiang Province 311300, China

Received:2024-03-15 Revised:2024-05-29 Published:2024-10-09

模拟牲畜采食对高寒草甸土壤潜在氧化亚氮排放的影响

吴晓芬^1,2, 陈有超^1,2, 蒋文婷^1,2, 沈玉叶^1,2, 蔡延江^1,2

1. 浙江农林大学省部共建亚热带森林培育国家重点实验室, 浙江杭州 311300;
2. 浙江农林大学环境与资源学院/碳中和学院, 浙江杭州 311300

通讯作者: 陈有超,E-mail:chenyouchao@zafu.edu.cn
作者简介:吴晓芬(1998-),女,汉族,浙江庆元人,硕士研究生,主要从事土壤学研究,E-mail:xiaofenwu993@163.com
基金资助:
国家自然科学基金(41877085，32371844)资助

Abstract

Abstract: Clarifying the impact of livestock defoliation on soil nitrous oxide (N₂O) emissions is pivotal for accurately predicting the alterations in greenhouse gas of the alpine meadow ecosystems under grazing disturbance. This study simulated the grazing behavior of livestock at different intensities by mowing stubble (no grazing,CK;light grazing,LD:stubble height 3 cm;heavy grazing,HD:stubble height ＜1 cm). By using incubation methods,we measured the potential N₂O emissions from the soil,and assessed soil physicochemical properties,enzyme activities,and the abundance of nitrification and denitrification functional genes. The results showed that the LD treatment had no significant effect on soil potential N₂O emissions,but the HD treatment significantly reduced potential N₂O emissions (P＜0.05). Heavy defoliation resulted in the decrease of ammonium nitrogen (NH₄⁺-N) input and carbon input,decreased the abundance of soil AOA-amoA,nirS and nosZI genes and denitrification potential (DP),weakened the nitrification and denitrification of soil,thereby reducing soil denitrification and N₂O emissions.In conclusion,light defoliation does not have a significant impact on potential N₂O emissions from alpine meadow soil,but heavy defoliation may reduce potential N₂O emissions by jointly reducing nitrification and denitrification in soil.

Key words: Alpine meadow, Nitrification, Denitrification, Grazing

摘要： 探明牲畜采食对土壤氧化亚氮(N₂O)排放的影响，是准确预测放牧干扰下高寒草甸生态系统温室气体变化的关键。本研究通过刈割留茬模拟牲畜不同强度采食行为(无采食，CK；轻度采食，LD：留茬高度3 cm；重度采食，HD：留茬高度＜1 cm)，利用室内培养法测定土壤潜在N₂O排放，并测定了土壤理化性质、酶活性以及硝化和反硝化功能基因丰度等指标。结果表明：LD处理对土壤潜在N₂O排放无显著影响，但HD处理显著降低了潜在N₂O排放(P＜0.05)。重度采食导致输入土壤铵态氮(NH₄⁺-N)和碳减少，降低土壤AOA-amoA,nirS和nosZI基因丰度及反硝化潜势(Denitrification potential,DP)，减弱土壤硝化和反硝化作用从而降低N₂O排放。综上，轻度采食对土壤潜在N₂O排放无显著影响，但重度采食可能通过降低土壤硝化和反硝化作用，减弱土壤潜在N₂O排放。

关键词: 高寒草甸, 硝化作用, 反硝化作用, 放牧

CLC Number:

S812.2

WU Xiao-fen, CHEN You-chao, JIANG Wen-ting, SHEN Yu-ye, CAI Yan-jiang. The Response of Potential N₂O Emission from an Alpine Meadow Soil to Simulated Livestock Grazing[J]. Acta Agrestia Sinica, 2024, 32(9): 2794-2802.

吴晓芬, 陈有超, 蒋文婷, 沈玉叶, 蔡延江. 模拟牲畜采食对高寒草甸土壤潜在氧化亚氮排放的影响[J]. 草地学报, 2024, 32(9): 2794-2802.

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References

[1] JONES M W,PETERS G P,GASSER T,et al. National contributions to climate change due to historical emissions of carbon dioxide,methane,and nitrous oxide since 1850[J]. Scientific Data,2023,10(1):155
[2] THROBÄCK I N,ENWALL K,JARVIS Å,et al. Reassessing PCR primers targeting nirS,nirK and nosZ genes for community surveys of denitrifying bacteria with DGGE [J]. FEMS Microbiology Ecology,2004,49(3):401-417
[3] ZHAN W,YANG Z,LIU J,et al. Effect of grazing intensities on soil N₂O emissions from an alpine meadow of Zoige Plateau in China[J]. Atmosphere,2021,12(5):541
[4] 马英,匡晓奎,刘杰,等.高寒草地土壤温室气体排放对放牧的响应研究进展[J]. 微生物学通报,2021,48(10):3835-3846
[5] 刘发央,龙瑞军. 不同放牧强度对牦牛夏季放牧行为的影响[J]. 兰州大学学报(自然科学版),2009,45(2):55-60
[6] 和占星,黄梅芬,雷波,等. 中国牦牛的生态行为研究进展[J]. 家畜生态学报,2019,40(4):1-9
[7] SKINNER R H,GOSLEE S C. Defoliation effects on pasture photosynthesis and respiration[J]. Crop Science,2016,56(4):2045-2053
[8] CHENG Y L,YANG Y,LI X,et al. Effects of simulated climate warming and grazing on photosynthesis and respiration of permafrost meadow plant community[J]. Russian Journal of Ecology,2020,51(3):224-232
[9] MIKOLA J,YEATES G W,BARKER G M,et al. Effects of defoliation intensity on soil food-web properties in an experimental grassland community[J]. Oikos,2001,92(2):333-343
[10] 张雅楠,王成杰,韩勇军,等. 施肥和刈割处理模拟放牧对西北针茅生长的影响[J]. 草地学报,2021,29(2):317-323
[11] WAN S,LUO Y. Substrate regulation of soil respiration in a tallgrass prairie:results of a clipping and shading experiment:substrate regulation of soil respiration[J]. Global Biogeochemical Cycles,2003,17(2):1054
[12] NEFTEL A,BLATTER A,SCHMID M,et al. An experimental determination of the scale length of N₂O in the soil of a grassland[J]. Journal of Geophysical Research:Atmospheres,2000,105(D10):12095-12103
[13] LEVY-BOOTH D J,PRESCOTT C E,GRAYSTON S J. Microbial functional genes involved in nitrogen fixation,nitrification and denitrification in forest ecosystems[J]. Soil Biology and Biochemistry,2014,75:11-25
[14] MARCHANT H K,AHMERKAMP S,LAVIK G,et al. Denitrifying community in coastal sediments performs aerobic and anaerobic respiration simultaneously[J]. Multidisciplinary Journal of Microbial Ecology,2017,11(8):1799-1812
[15] WELSH M K,MCMILLAN S K,VIDON P G. Denitrification along the stream-riparian continuum in restored and unrestored agricultural streams[J]. Journal of Environmental Quality,2017,46(5):1010-1019
[16] HART S C,STARK J M,DAVIDSON E A,et al. Nitrogen mineralization,immobilization,and nitrification[J]. Methods of Soil Analysis:Part 2 Microbiological and Biochemical Properties,1994,5:985-1018
[17] 张振,梁海彬,陈有超,等. 模拟家畜不同采食强度下高寒草甸土壤水解酶活性的变化特征[J]. 浙江农林大学学报,2023,40(2):265-273
[18] ZHONG L,DU R,DING K,et al. Effects of grazing on N₂O production potential and abundance of nitrifying and denitrifying microbial communities in meadow-steppe grassland in northern China[J]. Soil Biology and Biochemistry,2014,69:1-10
[19] YIN M,GAO X,KUANG W,et al. Soil N₂O emissions and functional genes in response to grazing grassland with livestock:a meta-analysis[J]. Geoderma,2023,436:116538
[20] CAO G,TANG Y,MO W,et al. Grazing intensity alters soil respiration in an alpine meadow on the Tibetan Plateau[J]. Soil Biology and Biochemistry,2004,36(2):237-243
[21] YANG Y,XIAO Y,LI C,et al. Nitrogen addition,rather than altered precipitation,stimulates nitrous oxide emissions in an alpine steppe[J]. Ecology and Evolution,2021,11(21):15153-15163
[22] 杨振安,姜林,徐颖怡,等. 青藏高原高寒草甸植被和土壤对短期禁牧的响应[J]. 生态学报,2017,37(23):7903-7911
[23] MA L,WANG Q,SHEN S T. Response of soil aggregate stability and distribution of organic carbon to alpine grassland degradation in northwest Sichuan[J]. Geoderma Regional,2020,22:e00309
[24] 门璐,何奕成,李婷婷,等. 高寒草甸植物叶绿素对氮添加梯度的非线性响应[J]. 草地学报,2023,31(6):1622-1631
[25] MA W,DING K,LI Z. Comparison of soil carbon and nitrogen stocks at grazing-excluded and yak grazed alpine meadow sites in Qnghai-Tibetan Plateau,China[J]. Journal of Ecological Engineering,2016,87:203-211
[26] LIU P,DING L,ZHOU Y,et al. Behavioural characteristics of yaks grazing summer and winter pastures on the Qinghai-Tibetan plateau[J]. Applied Animal Behaviour Science,2019,218:104826
[27] DING L,LONG R,YANG Y,et al. Behavioural responses by yaks in different physiological states (lactating,dry or replacement heifers),when grazing natural pasture in the spring (dry and germinating) season on the Qinghai-Tibetan Plateau[J]. Applied Animal Behaviour Science,2007,108(3-4):239-250
[28] QU F,YU J,DU S,et al. Influences of anthropogenic cultivation on C,N and P stoichiometry of reed-dominated coastal wetlands in the yellow river delta[J]. Geoderma,2014(235-236):227-232
[29] ZHANG H,FANG Y,CHEN Y,et al. Enhanced soil potential N₂O emissions by land-use change are linked to AOB-amoA and nirk gene abundances and denitrifying enzyme activity in subtropics[J]. Science of the Total Environment,2022,850:158032
[30] CAI Y,CHANG S X,MA B,et al. Watering increased DOC concentration but decreased N₂O emission from a mixed grassland soil under different defoliation regimes[J]. Biology and Fertility of Soils,2016,52(7):987-996
[31] 张海阔,张宝刚,周钟昱,等. 亚热带天然林转变为毛竹林和茶园对土壤胞外酶活性的影响[J]. 农业环境科学学报,2022,41(4):826-833
[32] GERMAN D P,WEINTRAUB M N,GRANDY A S,et al. Optimization of hydrolytic and oxidative enzyme methods for ecosystem studies[J]. Soil Biology and Biochemistry,2011,43(7):1387-1397
[33] TANG Y,ZHANG X,LI D,et al. Impacts of nitrogen and phosphorus additions on the abundance and community structure of ammonia oxidizers and denitrifying bacteria in Chinese fir plantations[J]. Soil Biology and Biochemistry,2016,103:284-293
[34] BOER W D,KOWALCHUK G A. Nitrification in acid soils:micro-organisms and mechanisms[J]. Soil Biology and Biochemistry,2001,33:853-866
[35] FRANCIS C A,ROBERTS K J,BEMAN J M,et al. Ubiquity and diversity of ammonia-oxidizing archaea in water columns and sediments of the ocean[J]. Proceedings of the National Academy of Sciences,2005,102(41):14683-14688
[36] ROTTHAUWE J H,WITZEL K P,LIESACK W. The ammonia monooxygenase structural gene amoA as a functional marker:molecular fine-scale analysis of natural ammonia-oxidizing populations[J]. Applied and Environmental Microbiology,1997,63(12):4704-4712
[37] HENRY S,BRU D,STRES B,et al. Quantitative detection of the nosZ gene,encoding nitrous oxide reductase,and comparison of the abundances of 16S rRNA,narG，nirK，and nosZ genes in soils[J]. Applied and Environmental Microbiology,2006,72(8):5181-5189
[38] 郭小伟,戴黎聪,李茜,等. 青藏高原放牧高寒草甸主要温室气体通量及其主控因素研究[J]. 草原与草坪,2019,39(3):72-78
[39] XIE Z,LE ROUX X,WANG C,et al. Identifying response groups of soil nitrifiers and denitrifiers to grazing and associated soil environmental drivers in Tibetan alpine meadows[J]. Soil Biology and Biochemistry,2014,77:89-99
[40] ZHANG L,HOU L,LAANBROEK H J,et al. Effects of mowing heights on N₂O emission from temperate grasslands in Inner Mongolia,Northern China[J]. American Journal of Climate Change,2015,4(5):397-407
[41] 李梓铭,杜睿,王亚玲,等. 中国草地N₂O通量日变化观测对比研究[J]. 中国环境科学,2012,32(12):2128-2133
[42] ZHANG K,LI M,YAN Z,et al. Changes in precipitation regime lead to acceleration of the N cycle and dramatic N₂O emission[J]. Science of the Total Environment,2022,808:152140

The Response of Potential N₂O Emission from an Alpine Meadow Soil to Simulated Livestock Grazing

模拟牲畜采食对高寒草甸土壤潜在氧化亚氮排放的影响

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