Abstract: Plant CCD7 gene is involved in the synthesis of strigolactones. In order to study the function of CCD7 gene in Psathyrostachys juncea, the tillering nodes of multi-tillering Psathyrostachys juncea and less-tillering P. juncea were used as materials. The expression of CCD7 gene in P. juncea was analyzed by RNA-seq and fluorescence quantitative PCR. The subcellular localization of CCD7 gene in tobacco leaves was analyzed by constructing pcambia 1300-cYFP-CCD7 overexpression vector. The basic functions of CCD7 gene were predicted by bioinformatics analysis.The results showed that the full length of P. juncea CCD7 gene sequence was 1 638 bp, and the subcellular localization of P. junceaCCD7 gene test found it was expressed in the chloroplast of tobacco leaf cells. The expression analysis showed that the tiller number of P. juncea was negatively correlated with the high expression of the gene. It was predicted that the conserved domain of CCD7 in P. juncea ranged from 13 to 544 amino acids. The gene was conserved in 9 closely related species, and the protein properties were similar. The serine phosphorylation site of CCD7 protein in P. juncea was most likely to be the phosphorylation site of protein function.

Key words: Psathyrostachys juncea, CCD7, Over expression vector, subcellular localization, Gene cloning

摘要： 植物CCD7基因参与合成独脚金内酯。为研究新麦草[Psathyrostachys juncea （Fisch.）Nevski]CCD7基因的功能,本研究以多分蘖型新麦草和少分蘖型新麦草的分蘖节为材料,通过RNA-seq及荧光定量PCR分析CCD7基因在新麦草中的表达量,通过构建pcambia1300-cYFP-CCD7过表达载体,分析新麦草CCD7基因在烟草叶片中的亚细胞定位；通过生物信息学分析预测CCD7基因的基本功能。结果显示,新麦草CCD7基因序列克隆全长为1638 bp,亚细胞定位新麦草CCD7基因在烟草叶片细胞的叶绿体中表达；表达量分析表明新麦草的分蘖数量与该基因的表达量呈现负相关关系。预测新麦草CCD7保守结构域的范围是第13到第544个氨基酸,该基因在9个近缘物种中功能保守,编码蛋白性质相近,新麦草CCD7蛋白的丝氨酸磷酸化位点最有可能是蛋白发挥功能的磷酸化位点。

关键词: 新麦草, CCD7, 过表达载体, 亚细胞定位, 基因克隆