Screening of Drought-Tolerant Germplasm Resources of White Clover and Analysis of Drought Tolerance Evaluation Index

doi:10.11733/j.issn.1007-0435.2025.05.017

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (5): 1510-1522.DOI: 10.11733/j.issn.1007-0435.2025.05.017

Previous Articles Next Articles

Screening of Drought-Tolerant Germplasm Resources of White Clover and Analysis of Drought Tolerance Evaluation Index

YANG Wei-qiang, ZHANG Han, JIANG Ji-shun, QI Tian-gang, ZHOU Qin-yu, PENG Yan

College of Grassland Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan Province 611130, China

Received:2024-08-02 Revised:2024-10-11 Online:2025-05-15 Published:2025-05-20

白三叶抗旱种质资源筛选及抗旱评价指标筛选

杨维强, 张晗, 姜吉顺, 齐天罡, 周沁屿, 彭燕

四川农业大学草业科技学院, 四川成都 611130

通讯作者: 彭燕,E-mail:pengyanlee@163.com
作者简介:杨维强（1999-），男，汉族，甘肃天水人，硕士研究生，主要从事草坪草抗逆研究，E-mail：yweiqiang2023@163.com
基金资助:
自然基金项目：32471769；四川创新团队项目：SCCXTD-2025-16资助

Abstract

Abstract: To identify white clover （Trifolium repens） germplasm resources with exceptional drought resistance and to select effective indicators for evaluating this trait， the present study was conducted using 110 T. repens germplasm resources from natural distributions in Sichuan and other regions， along with the nationally recognized cultivars ‘Haifa’ and ‘Goethe’ as test materials. Under natural drought conditions in the field， a preliminary evaluation of drought resistance was performed by observing the differences in phenotypic indices of the test materials. In controlled indoor conditions， the weighted subordinate function method was employed to assess the drought resistance of the initially screened materials by examining changes in the cross-sectional structure of the leaf blades and various physiological parameters. Correlation and principal component analyses were utilized to evaluate the contribution rate of each index， which facilitated the identification of suitable indicators for assessing the drought resistance of white clover. The results indicated a close relationship between the morphological characteristics of white clover materials and their drought tolerance， as revealed by cluster analysis. The comprehensive evaluation identified materials Tr210， Tr103， and Tr083 as having strong drought tolerance， while Tr008 and Tr033 exhibited poor drought tolerance. Among the 26 evaluation indices， 16 effective indices were ultimately selected， comprising 4 phenotypic indices， 8 physiological indices， and 4 leaf microstructure indices. This study provides a theoretical foundation and material basis for the evaluation of drought-tolerant germplasm resources， as well as for the selection and breeding of drought-resistant varieties of white clover.

Key words: Trifolicem repens, Drought resistance, Drought resistance index, Comprehensive evaluation

摘要： 为发掘抗旱性优异的白三叶（Trifolium repens）种质资源以及筛选评价其抗旱性强弱的有效指标，本试验以采自四川等地自然分布的110份白三叶种质资源以及国审品种‘Haifa’‘Goethe’为试验材料，在田间自然干旱条件下，通过观测供试材料的表型指标差异对其抗旱性初步评价；在室内控水条件下，通过观测叶片横切结构和生理参数的变化，运用加权隶属函数法对初筛材料进行抗旱性鉴定。采用相关性和主成分分析方法评价各指标的贡献率以筛选出适宜白三叶抗旱性评价的指标。结果表明：聚类分析显示白三叶的形态特征和抗旱性密切相关；综合评价表明，材料Tr210，Tr103，Tr083具有较强耐旱性，而Tr008和Tr033耐旱性较差。在26个评价指标中，最终筛选出16个有效指标，包括4个表型指标、8个生理指标和4个叶片显微结构指标。本研究为白三叶耐旱种质资源评价以及耐旱品种选育提供了理论依据和材料基础。

关键词: 白三叶, 抗旱性, 抗旱指标, 综合评价

CLC Number:

S541.2

YANG Wei-qiang, ZHANG Han, JIANG Ji-shun, QI Tian-gang, ZHOU Qin-yu, PENG Yan. Screening of Drought-Tolerant Germplasm Resources of White Clover and Analysis of Drought Tolerance Evaluation Index[J]. Acta Agrestia Sinica, 2025, 33(5): 1510-1522.

杨维强, 张晗, 姜吉顺, 齐天罡, 周沁屿, 彭燕. 白三叶抗旱种质资源筛选及抗旱评价指标筛选[J]. 草地学报, 2025, 33(5): 1510-1522.

References

[1] MONFORTE A J，DIAZ A，CAÑO-DELGADO A，et al. The genetic basis of fruit morphology in horticultural crops：lessons from tomato and melon[J]. Journal of Experimental Botany，2014，65（16）：4625-4637
[2] KNOWLES I M，FRASER T J，DALY M J. White clover：loss in drought and subsequent recovery[J]. NZGA：Research and Practice Series，2003（11）：37-41
[3] CLIFFORD P T P，BAIRD I J，GRBAVAC N，et al. White clover soil seed loads：effect on requirements and resultant success of cultivar-change crops[J]. Proceedings of the New Zealand Grassland Association，1990（52）：95-98
[4] HOYOS-VILLEGAS V，O’CONNOR J R，HESLOP A D，et al. Rate of genetic gain for persistence to grazing and dry matter yield in white clover across 90 years of cultivar development[J]. Crop Science，2019，59（2）：537-552
[5] 杜逸，郑德成，王夭群. 野生白三叶生态适应性的调查[J]. 中国草原，1983，5（4）：37-40
[6] 朱邦长. 中国的白三叶草资源[J]. 草业科学， 1986，3（1）：4-9
[7] SINGH D K，SALE P W G，PALLAGHY C K，et al. Phosphorus concentrations in the leaves of defoliated white clover affect abscisic acid formation and transpiration in drying soil[J]. New Phytologist，2000，146（2）：249-259
[8] WOODFIELD D R，CARADUS J R. Adaptation of white clover to moisture stress[J]. Proceedings of the New Zealand Grassland Association，1987（48）：143-149
[9] LI Z，JING W，PENG Y，et al. Spermine alleviates drought stress in white clover with different resistance by influencing carbohydrate metabolism and dehydrins synthesis[J]. PLos One，2015，10（3）：e0120708
[10] BARRS H D，WEATHERLEY P E. A re-examination of the relative turgidity technique for estimating water deficits in leaves[J]. Australian Journal of Biological Sciences，1962，15（3）：413
[11] 王玉祥，陈述明，张博. 苜蓿根尖制片技术研究[J]. 中国农学通报，2013，29（9）：163-166
[12] ARNON D I. Copper enzymes in isolated chloroplasts. polyphenoloxidase in beta vulgaris[J]. Plant Physiology，1949，24（1）：1-15
[13] BLUM A，EBERCON A. Cell membrane stability as a measure of drought and heat tolerance in Wheat1[J]. Crop Science，1981，21（1）：43-47
[14] 郝俊峰，张玉霞，贾玉山，等. PEG-6000胁迫下苜蓿萌发期抗旱性鉴定与评价[J]. 西北农林科技大学学报（自然科学版），2020，48（11）：23-32
[15] WANG X G，DENG Y S，GAO L Y，et al. Series-temporal transcriptome profiling of cotton reveals the response mechanism of phosphatidylinositol signaling system in the early stage of drought stress[J]. Genomics，2022，114（5）：110465
[16] GOTOH E，SUETSUGU N，HIGA T，et al. Palisade cell shape affects the light-induced chloroplast movements and leaf photosynthesis[J]. Scientific Reports，2018，8（1）：1472
[17] 杨特武，鲍健寅，何光明，等. 干旱胁迫下白三叶器官生理特性变化及其SOD在抗旱中的作用[J]. 中国草地，1997，19（4）：55-61
[18] 刘军，齐广平，康燕霞，等. 不同灌溉处理下紫花苜蓿光合特性、叶绿素荧光参数及生物量的变化[J]. 草地学报，2019，27（6）：1569-1576
[19] 郭颖，韩蕊莲，梁宗锁. 土壤干旱对黄土高原4个乡土禾草生长及水分利用特性的影响[J]. 草业学报，2010，19（2）：21-30
[20] BISSUEL-BELAYGUE C，COWAN A A，MARSHALL A H，et al. Reproductive development of white clover （Trifolium repens L.） is not impaired by a moderate water deficit that reduces vegetative growth：ii. fertilization efficiency and seed set[J]. Crop Science，2002，42（2）：414-422
[21] GONZÁLEZ A P R，DUMALASOVÁ V，ROSENTHAL J，et al. The role of transgenerational effects in adaptation of clonal offspring of white clover （Trifolium repens） to drought and herbivory[J]. Evolutionary Ecology，2017，31（3）：345-361
[22] ZHAO S Y，ZENG W H，LI Z，et al. Mannose regulates water balance，leaf senescence，and genes related to stress tolerance in white clover under osmotic stress[J]. Biologia Plantarum，2020（64）：406-416
[23] LIU F L，ANDERSEN M N，JACOBSEN S E，et al. Stomatal control and water use efficiency of soybean （Glycine max L. Merr.） during progressive soil drying[J]. Environmental and Experimental Botany，2005，54（1）：33-40
[24] LOBATO A，COSTA R C，NETO C，et al. Consequences of the water deficit on water relations and symbiosis in Vigna unguiculata cultivars[J]. Plant Soil and Environment，2018（55）：139-145
[25] LI Z，GENG W，TAN M，et al. Differential responses to salt stress in four white clover genotypes associated with root growth，endogenous polyamines metabolism，and sodium/potassium accumulation and transport[J]. Frontiers in Plant Science，2022（13）：896436
[26] 崔维佳，常志云，李宁. 干旱胁迫对冬小麦生态、光合、产量及光谱特征的影响[J]. 山西农业科学，2013，41（12）：1314-1318，1346
[27] 何苗，姜元昊，轩正英，等.芜菁幼苗叶片光合特性和解剖结构对干旱胁迫的响应[J].西北植物学报，2024，44（10）：1530-1538
[28] APEL K，HIRT H. Reactive oxygen species：metabolism，oxidative stress，and signal transduction[J]. Annual Review of Plant Biology，2004（55）：373-399
[29] 季杨，张新全，彭燕，等. 干旱胁迫对鸭茅根、叶保护酶活性、渗透物质含量及膜质过氧化作用的影响[J]. 草业学报，2014，23（3）：144-151
[30] MITTLER R. Oxidative stress，antioxidants and stress tolerance[J]. Trends in Plant Science，2002，7（9）：405-410
[31] 张一龙，孙晓梵，李硕，等. 不同抗旱性狗牙根种质的抗旱生理响应差异分析[J]. 中国农业科技导报，2023，25（6）：59-70
[32] LIU Z Q，LIU T，LIANG L L，et al. Enhanced photosynthesis，carbohydrates，and energy metabolism associated with chitosan-induced drought tolerance in creeping bentgrass[J]. Crop Science，2020，60（2）：1064-1076
[33] VASEVA I，AKISCAN Y，SIMOVA-STOILOVA L，et al. Antioxidant response to drought in red and white clover[J]. Acta Physiologiae Plantarum，2012，34（5）：1689-1699
[34] DONG X J，ZHANG X S. Some observations of the adaptations of sandy shrubs to the arid environment in the mu us sandland：leaf water relations and anatomic features[J]. Journal of Arid Environments，2001，48（1）：41-48
[35] 李广毅，高国雄，吕悦来，等. 三种灌木植物形态特征及解剖结构的对比观察[J]. 水土保持研究，1995，2（2）：141-145
[36] 张德巧，徐增莱，褚晓芳，等. 蓝莓叶片与抗旱性相关的解剖结构指标研究[J]. 果树学报，2008，25（6）：864-867
[37] 常英俏，徐文远，穆立蔷，等. 干旱胁迫对3种观赏灌木叶片解剖结构的影响及抗旱性分析[J]. 东北林业大学学报，2012，40（3）：36-40
[38] 李正理. 旱生植物的形态和结构[J]. 生物学通报，1981，16（4）：9-12
[39] 董蕾，陈博，李吉跃，等. 5个楸树无性系抗旱性的综合评价[J]. 中南林业科技大学学报，2014，34（4）：30-35
[40] 潘昕，邱权，李吉跃，等. 基于叶片解剖结构对青藏高原25种灌木的抗旱性评价[J]. 华南农业大学学报，2015，36（2）：61-68
[41] 路贵和，戴景瑞，张书奎，等. 不同干旱胁迫条件下我国玉米骨干自交系的抗旱性比较研究[J]. 作物学报，2005，31（10）：1284-1288
[42] 祁旭升，刘章雄，关荣霞，等. 大豆成株期抗旱性鉴定评价方法研究[J]. 作物学报，2012，38（4）：665-674
[43] 王兰芬，武晶，景蕊莲，等. 绿豆种质资源苗期抗旱性鉴定[J]. 作物学报，2015，41（1）：145-153
[44] 王焱，沙柏平，李明雨，等. 苜蓿种质资源萌发期抗旱指标筛选及抗旱性综合评价[J]. 植物遗传资源学报，2019，20（3）：598-609，623
[45] 张灿军，冀天会，杨子光，等. 小麦抗旱性鉴定方法及评价指标研究Ⅰ 鉴定方法及评价指标[J]. 中国农学通报，2007，23（9）：226-230
[46] 李怡，侯向阳，武自念，等. 羊草种质资源抗旱性综合评价[J]. 中国草地学报，2019，41（1）：75-82

Screening of Drought-Tolerant Germplasm Resources of White Clover and Analysis of Drought Tolerance Evaluation Index

白三叶抗旱种质资源筛选及抗旱评价指标筛选

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	SHI Jing, LIU Xiao-jing, LI Jia-qi, HAN Yan-long, WANG Xue, WANG Jing. Study on the Adaptability of 15 Alfalfa Varieties in Minqin Area [J]. Acta Agrestia Sinica, 2025, 33(9): 2843-2853.
[2]	JIN Jian-gang, ZHENG Min-na, KANG Jia-hui, YANG Fu, TIAN Zai-fang, WANG Yuan-dong. Comprehensive Evaluation of the Stem Lodging Resistance Traits of 98 Oat Varieties （lines） [J]. Acta Agrestia Sinica, 2025, 33(8): 2567-2574.
[3]	LIU Tian, FENG Jia-xin, LIU Meng-jiao, Chen Jing-bo, GUO Hai-lin, KONG Wei-yi, ZHENG Yi-qi. Screening and Comprehensive Evaluation of Shade-Tolerance Hybrid Cultivars in Zoysiagrass （Zoysia spp.） [J]. Acta Agrestia Sinica, 2025, 33(7): 2288-2298.
[4]	LYU Yan-zhen, YAO Xing-jie, ZHANG Zhao, SUN Qing-ying, YANG Yu-ze, YAN Hui-fang. Evaluation of Alkali Tolerance and Key Indicators Screening during Seed Germination of Different Alfalfa Cultivars [J]. Acta Agrestia Sinica, 2025, 33(6): 1852-1861.
[5]	YANG Bing, WANG Yu-ting, LIU Jin-long, HAN Xu, LI Yue, SUN Yan. Comprehensive Evaluation and Screening of Lodging Resistance of Different Oat Varieties [J]. Acta Agrestia Sinica, 2025, 33(5): 1408-1417.
[6]	ZHU Qi-meng, HE Lin-lin, WU Ting-da, ZHANG Chi, RAN Fu, LI Juan-xia, YAN Yu-bang, ZHU Ya-nan, ZHANG Cai-zhong, BAI Xiao-ming. Comprehensive Evaluation on Salt Stress of 10 Wild Ophiopogoneae Germplasms in Southern Region of Gansu Province [J]. Acta Agrestia Sinica, 2025, 33(5): 1588-1601.
[7]	YAO Xing-jie, SUN Qing-ying, ZHANG Zhao, LYU Yan-zhen, YAN Si-qi, CHEN Si-yu, SHI Yan-xin, DUAN Xiao-tong, MENG Shu-han, YAN Hui-fang. Comprehensive Evaluation on Salt Tolerance of 61 Alfalfa Cultivars during Seed Germination [J]. Acta Agrestia Sinica, 2025, 33(4): 1181-1191.
[8]	SHI Jia-qi, XIE Nan, CUI Sui-qian, SUN Guo-tong, PAN Xuan, ZHANG Li-feng, LIU Zhong-kuan, LIU Zhen-yu, ZHANG Hong-fa, LI Ya-nan, YANG Qing-chuan, LONG Rui-cai. Evaluation of Salt Tolerance of 16 Alfalfa Varieties under Different Salt Concentration Stress at Germination Stage [J]. Acta Agrestia Sinica, 2025, 33(2): 472-480.
[9]	LI Chun-yan, ZHANG Ran-ran, ZHU Hai-yan, YOU Dong-mei, HU Hai-yan, LI Zong-zhen, RONG Yu-ping. Effects of Salt Stress on Physiological Characteristics of Alfalfa Seedlings and Evaluation of Salt Tolerance at Seedling Stage [J]. Acta Agrestia Sinica, 2025, 33(2): 516-523.
[10]	FU Jun-shi, NAN Li-li, ZHANG Ze-long, WU Shi-wen, REN Meng-yu, CHEN Na, JIAO Long-guang. Comprehensive Evaluation of Agronomic and Quality Traits of Timothy Germplasm Resources [J]. Acta Agrestia Sinica, 2025, 33(11): 3633-3642.
[11]	XU Zhao-qi, LI Xiao, CHEN Yong-cheng, ZHANG Fan-fan, MA Chun-hui. Effects of Drought Stress on Physiological and Photosynthetic Characteristics of Urtica cannabina [J]. Acta Agrestia Sinica, 2025, 33(10): 3237-3244.
[12]	LI Cui, YANG Zhi-chen, GUO Chen-jia, ZHENG Mei, ZHAO Chun-qiao, HOU Xin-cun, GUO Qiang. Evaluation and Identification of Salt Tolerance of 22 Karelinia caspia Germplasm [J]. Acta Agrestia Sinica, 2025, 33(10): 3353-3361.
[13]	LIU Ze-yu, YUAN Zi-tong, SUI Ke, ZHANG Yu-xia, LIU Ting-yu, SHAN Xin-he, ZHANG Zhi-yong, WANG Xian-guo. Comprehensive Evaluation of Cold Resistance of Alfalfa Varieties of the “WL” Series Based on Cold Resistance Coefficients [J]. Acta Agrestia Sinica, 2024, 32(8): 2592-2598.
[14]	MA Xue-jun, ZUO Yu-zhen, LIU Rui-gang, CHEN Ping, XIE Jian-peng, WANG Fei, HE Zhen-fu. Evaluation of Productive Performance and Nutritional Value of 20 Silage Maize Varieties in Southeastern Gansu [J]. Acta Agrestia Sinica, 2024, 32(8): 2620-2629.
[15]	DONG Shuang-hui, JIA Ming, ZHANG Hui, TENG Ke, TENG Wen-jun, WEN Hai-feng, FAN Xi-feng, YUE Yue-sen, WU Ju-ying, DING Guo-chang. The Physiological Response and Tolerance Evaluation of Tetraploid Carex breviculmis to Low Temperature [J]. Acta Agrestia Sinica, 2024, 32(7): 2089-2098.