Temporal Effects of Artificially Assisted Restoration on Soil Physicochemical Properties and Soil Microorganisms of Grasslands in the Sanjiangyuan Area

doi:10.11733/j.issn.1007-0435.2025.07.013

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (7): 2172-2183.DOI: 10.11733/j.issn.1007-0435.2025.07.013

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Temporal Effects of Artificially Assisted Restoration on Soil Physicochemical Properties and Soil Microorganisms of Grasslands in the Sanjiangyuan Area

SUN Ran^1,4, QI Xue-zhong², KAZHUO cairang², ZHOU Hua-kun³, LI Hong-lin⁴

1. College of Eco-Environmental Engineering, Qinghai University, Xining, Qinghai Province 810016, China;
2. Grassland Station in Henan Mongolis Autonomous County, Huangnan, Qinghai Province 811599, China;
3. Key Laboratory of Restoration Ecology for Cold Regions Laboratory in Qinghai, Northwest Institute of Plateau Biology, Chinese Academy of Science, Xinning, Qinghai Province 81008, China;
4. State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, Qinghai Province 810016, China

Received:2024-09-05 Revised:2024-11-08 Online:2025-07-15 Published:2025-07-18

人工辅助恢复对三江源区草地土壤理化性质和土壤微生物的时间影响

孙冉^1,4, 祁学忠², 卡着才让², 周华坤³, 李宏林⁴

1. 青海大学生态与环境工程学院, 青海西宁 810016;
2. 河南蒙古族自治县草原工作站, 青海黄南州 811599;
3. 中国科学院西北高原生物研究所, 青海省寒区恢复生态学重点实验室, 青海西宁 810008;
4. 青海大学省部共建三江源生态与高原农牧业国家重点实验室, 青海西宁 810016

通讯作者: 李宏林,E-mail:honglinli@qhu.edu.cn
作者简介:孙冉（1999-），女，汉族，河南项城人，硕士研究生，主要从事修复生态学研究，E-mail：13099748315@163.com
基金资助:
青海省科学技术厅项目（2023-ZJ-743），国家自然科学基金联合基金项目（U21A20186）；中央财政林业科技推广示范项目（青〔2023〕TG22）资助

Abstract

Abstract: In order to investigate the influences of restoration period on microbial and physicochemical properties of soil in artificially restored grassland in the Sanjiangyuan area， the changing rules of soil physicochemical properties and microbial communities under four restoration periods （2， 5， 8 and 10 a） were analyzed by taking the artificially restored grassland in Henan and Mongolia Autonomous County， Qinghai Province， as the study object. The results demonstrated a decreasing tendency firstly for soil nutrients， including soil organic carbon， total nitrogen， available phosphorus and ammonium nitrogen， and then increased with the prolongation of the years of restoration of the artificial restored grassland. The bacterial community exhibited the greatest diversity in the grassland soil following two years of restoration， while the fungi demonstrated the highest diversity following ten years of restoration. At the phylum level， the dominant bacterial phyla were Proteobacteria， Actinobacteria， Bacteroidota， Acidobacteriota and Gemmatimonadota， collectively accounting for 85.98%. The dominant fungal phyla were Ascomycota and Basidiomycota， which collectively accounted for 83.98% of the total relative abundance. At the genus level， the abundance of most beneficial bacteria exhibited an increasing trend with the extension of recovery years， including Sphingomona and Mortierella， as well as other beneficial genera. This study found that by constructing artificial grassland could increase soil nutrients and improve soil microbial community structure of degraded grassland in general， which could provide a basis for the scientific management of artificially restored grassland in Sanjiangyuan District.

Key words: Artificially assisted restoration of grassland, Years of restoration, Soil physicochemical properties, Microbial community

摘要： 为探究恢复年限对三江源区人工辅助恢复草地土壤微生物和理化性质的影响，以河南蒙古族自治县恢复草地为研究对象，分析四种恢复年限（2，5，8和10 a）下土壤理化性质和微生物群落的变化。结果表明：随着人工辅助恢复草地恢复年限的增加，土壤有机碳、全氮、硝态氮和铵态氮等土壤养分呈现先减少后增加趋势；细菌群落在恢复2年的草地土壤中多样性最高，真菌群落在恢复10 a最高；优势细菌门依次为变形菌门、放线菌门、拟杆菌门、酸杆菌门和芽单胞菌门，占总相对丰度的85.98%；优势真菌门为子囊菌门和担子菌门，占总相对丰度的83.03%；在属水平上，大部分有益菌，如鞘氨醇单胞菌属、被孢霉属丰度随着年限增加呈上升趋势。研究发现人工补播能够增加退化草地土壤有机碳、全氮、速效磷和铵态氮、改善土壤微生物群落结构，从而为修复草地生态提供科学依据。

关键词: 人工辅助恢复草地, 恢复年限, 土壤理化性质, 微生物群落

CLC Number:

S154.4

SUN Ran, QI Xue-zhong, KAZHUO cairang, ZHOU Hua-kun, LI Hong-lin. Temporal Effects of Artificially Assisted Restoration on Soil Physicochemical Properties and Soil Microorganisms of Grasslands in the Sanjiangyuan Area[J]. Acta Agrestia Sinica, 2025, 33(7): 2172-2183.

孙冉, 祁学忠, 卡着才让, 周华坤, 李宏林. 人工辅助恢复对三江源区草地土壤理化性质和土壤微生物的时间影响[J]. 草地学报, 2025, 33(7): 2172-2183.

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Temporal Effects of Artificially Assisted Restoration on Soil Physicochemical Properties and Soil Microorganisms of Grasslands in the Sanjiangyuan Area

人工辅助恢复对三江源区草地土壤理化性质和土壤微生物的时间影响

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