Identification and Expression Analysis of XTH Gene Family in Different Developmental Stages in Cannabis sativa

doi:10.11733/j.issn.1007-0435.2025.08.004

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (8): 2443-2455.DOI: 10.11733/j.issn.1007-0435.2025.08.004

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Identification and Expression Analysis of XTH Gene Family in Different Developmental Stages in Cannabis sativa

SUI Yue, YAN Bo-wei, ZHANG Yuan-ye, FANG Yu-yan, ZHENG Nan, ZHANG Ming, ZHANG Li-guo

Industrial Crops Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang Province 150086, China

Received:2024-11-13 Revised:2024-12-04 Online:2025-08-15 Published:2025-08-27

工业大麻XTH基因家族鉴定及不同发育时期表达模式分析

隋月, 闫博巍, 张元野, 房郁妍, 郑楠, 张明, 张利国

黑龙江省农业科学院经济作物研究所, 黑龙江哈尔滨 150086

通讯作者: 张利国, E-mail:zlg86@aliyun.com
作者简介:隋月(1997-),女,汉族,黑龙江哈尔滨人,硕士,助理研究员,主要从事工业大麻遗传育种研究,E-mail:suiyuework@163.com
基金资助:
黑龙江省农业科技创新跨越工程—经济作物突破性新品种选育及产业化应用（GX23GG04）资助

Abstract

Abstract: Xyloglucan endotransglycosylases/hydrolases （XTH） are a class of enzymes involved in the construction and remodeling of cellulose/xyloglucan crosslinks and play an important role in regulating plant cell wall extensibility. However， these enzymes have not been reported in Cannabis sativa. In order to systematically identify the XTH gene family members of the C. sativa， 10 XTH genes （CsXTH） in C. sativa were identified by bioinformatics methods， named CsXTH1 to CsXTH10， respectively. Their physical-chemical properties， phylogenetic evolution and expression patterns at different growth stages were analyzed. The results showed that the relative molecular weights of XTH family members ranged from 31.14 to 63.64 kD. Subcellular localization prediction results showed that all XTH proteins were located on the cell wall， with CsXTH3 and CsXTH8 could also be located in the cytoplasm， which were all hydrophilic proteins. There are many cis-acting regulatory elements related to light response， hormone response and stress response in the promoter region of CsXTH， suggesting that CsXTH may be involved in plant growth and development， stress defense and other biological processes. The expression of CsXTH genes was specific in different growth stages. The expression levels of CsXTH3 and CsXTH5 were the highest in the seeding stage， and CsXTH7 was the highest in the process maturity. The above analysis provided a useful reference for the further validation of CsXTH family genes in the development of fiber in C. sativa.

Key words: Cannabis sativa, XTH gene family, Fiber development, Bioinformatics analysis

摘要： 木葡聚糖内转糖苷酶/水解酶（Xyloglucan endotransglycosylases/hydrolases， XTH）参与纤维素/木葡聚糖交联构建和重塑，在植物细胞壁的可伸展性方面有非常重要的作用。但在工业大麻（Cannabis sativa）中还未有关于该酶的报道。为系统鉴定工业大麻XTH家族成员，本研究通过生物信息学手段，鉴定出10个工业大麻XTH基因（CsXTH），分别命名为CsXTH1~CsXTH10，分析了其理化性质、系统进化和不同生长时期表达模式等。结果表明，XTH家族成员相对分子量在31.14~63.64 kD之间。亚细胞定位预测结果显示，10个家族成员均定位在细胞壁上，CsXTH3和CsXTH8也能定位在细胞质中，均属于亲水性蛋白。CsXTH启动子区存在较多光响应、激素响应和逆境响应相关的顺式作用元件，表明CsXTH可能参与植物生长发育及逆境胁迫防御等多种生物学过程。CsXTH基因的表达在不同生长时期存在特异性，CsXTH3，CsXTH5在幼苗期表达量最高，CsXTH7在工艺成熟期表达量最高。上述结果为研究CsXTH家族基因在工业大麻纤维发育中的功能提供了参考依据。

关键词: 工业大麻, XTH基因家族, 纤维发育, 生物信息学分析

CLC Number:

S563.3
Q78

SUI Yue, YAN Bo-wei, ZHANG Yuan-ye, FANG Yu-yan, ZHENG Nan, ZHANG Ming, ZHANG Li-guo. Identification and Expression Analysis of XTH Gene Family in Different Developmental Stages in Cannabis sativa[J]. Acta Agrestia Sinica, 2025, 33(8): 2443-2455.

隋月, 闫博巍, 张元野, 房郁妍, 郑楠, 张明, 张利国. 工业大麻XTH基因家族鉴定及不同发育时期表达模式分析[J]. 草地学报, 2025, 33(8): 2443-2455.

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Identification and Expression Analysis of XTH Gene Family in Different Developmental Stages in Cannabis sativa

工业大麻XTH基因家族鉴定及不同发育时期表达模式分析

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