Acta Agrestia Sinica

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (1): 118-131.DOI: 10.11733/j.issn.1007-0435.2026.01.012

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Effects Of Exogenous ABA on Metabolites of Wild Medicinal-Edible Plants Sphallerocarpus gracilis in Qilian Mountains

ZHANG Chun-mei1,2, YAN Fang2,3, ZHANG Xi-feng1,2, QI Shi-ming1,2, ZHAO Gang1,2, SONG Hai2, CHEN Ye1,2, LIU Zhen-rong1,2, LI Xin-ru1,2   

  1. 1. College of Agriculture and Ecological Engineering, Hexi University, Zhangye, Gansu Province 734000, China;
    2. Key Laboratory of Hexi Corridor Resources Utilization of GanSu, Zhangye, Gansu Province 734000, China;
    3. Ecological & Oasis Agricultural Research Institute of Hexi University, Zhangye, Gansu Province 734000, China
  • Received:2025-02-14 Revised:2025-05-08 Published:2025-12-24

外源脱落酸对祁连山野生药食植物黄参代谢物的影响

张春梅1,2, 闫芳2,3, 张喜峰1,2, 祁世明1,2, 赵刚1,2, 宋海2, 陈叶1,2, 刘振荣1,2, 李欣孺1,2   

  1. 1. 河西学院农业与生态工程学院, 甘肃 张掖 734000;
    2. 甘肃省河西走廊特色资源利用重点实验室, 甘肃 张掖 734000;
    3. 河西学院生态与绿洲农业研究院, 甘肃 张掖 734000
  • 作者简介:张春梅（1978-），女，汉族，甘肃酒泉人，教授，博士，主要从事植物生理及生物技术研究，*E-mail:zazcm197828@163.com
  • 基金资助:
    国家自然科学基金项目（No.32160745）；甘肃省自然科学基金项目（No.22JR5RG566）；河西学院2024年“挑战杯”竞赛培育项目（7）；河西学院第十五批大学生科技创新项目（Z57）资助

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Abstract: This study aims to explore the effects of exogenous ABA on the accumulation pattern of differential metabolites in the roots of wild medicinal-edible plants Sphallerocarpus gracilis of Qilian Mountains. Ultra-high liquid phase system and ultra-high performance liquid chromatography-electrospray-triple quadrupole linear ion trap-tandem mass spectrometry （UPLC-ESI-QTRAP-MS/MS） extensive targeted metabolomics technology was used to detect and analyze the changes of metabolites in roots by applying with 20 μg·L-1 exogenous abscisic acid （ABA） as well as 2000 mg·L-1 sodium tungstate. The results showed that two kinds of carotenoids were detected in the roots of Sphallerocarpus gracilis for the first time， they were β-carotene and lutein， respectively. Furthermore， a total of 537 differential DAMs in 7 categories were identified， among them shikimic acid and phenylpropionic acid （206 species）， terpenoids（93 species）， alkaloids （92 species）and amino acids （84 species） accounted for a relatively high proportion. Pathway enrichment analysis was performed on differential metabolites based on Kyoto Encyclopedia of Genes and Genomes. The differential metabolites （DAMs） were distributed in 15 metabolic pathways in 5 categories， which were mainly enriched in phenylalanine metabolism， thiamine metabolism and tyrosine metabolism. In summary， exogenous ABA and its inhibitor sodium tungstate can affect the metabolic pattern of Sphallerocarpus gracilis， which is expected to be applied to the development of targeted regulators on key metabolic pathways.

Key words: Sphallerocarpus gracilis, Metabonomics, Abscisic acid, Differential metabolites, Pathway enrichment analysis

摘要： 为揭示外源脱落酸（Abscisic acid，ABA）对祁连山野生药食植物黄参（Sphallerocarpus gracilis）肉质根中代谢物积累模式的影响，本研究采用超高效液相系统和UPLC-电喷雾（Electron spray ionization，ESI）-三重四极杆线性离子阱（Triple quadrupole-linear ion trap，QTRAP）-MS/MS的广泛靶向代谢组学技术，探究了施用20 μg·L-1ABA和2000 mg·L-1ABA抑制剂钨酸钠时根中代谢物的变化。本研究首次从黄参肉质根中鉴定出两种类胡萝卜素，分别为β-胡萝卜素和叶黄素；共得到7类537 种差异代谢物，其中莽草酸及苯丙酸类（206种）、萜类（93种）、生物碱（92种）和氨基酸及短肽（84种）占比较高；京都基因与基因组百科全书（Kyoto encyclopedia of genes and genomes，KEGG）数据库通路富集分析表明，差异代谢物分布在5大类15条代谢途径中，主要富集在苯丙氨酸代谢、硫胺素代谢和酪氨酸代谢等通路。综上所述，外源ABA及其抑制剂会影响黄参肉质根代谢模式，ABA有望用于开发关键代谢通路的靶向调控剂。

关键词: 黄参, 代谢组学, 脱落酸, 差异代谢物, 通路富集分析

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