Genome-Wide Identification and Cold Stress Expression Analysis of the WRKY Gene Family in Pennisetum alopecuroides

doi:10.11733/j.issn.1007-0435.2026.09.006

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (9): 3197-3210.DOI: 10.11733/j.issn.1007-0435.2026.09.006

Genome-Wide Identification and Cold Stress Expression Analysis of the WRKY Gene Family in Pennisetum alopecuroides

SHI Zhi-fu^1,2, WANG Xin², YUE Yue-sen², TENG Ke², ZHOU Le², LIU Ling-yun², YUAN Su-fan², FAN Xi-feng², MU Na²

1. Beijing University of Agriculture, Beijing 102206, China;
2. Institute of Grassland, Flowers and Landscape Ecology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China

Received:2026-03-11 Revised:2026-04-15 Published:2026-09-11

狼尾草WRKY基因家族全基因组鉴定与冷胁迫表达分析

史志福^1,2, 王鑫², 岳跃森², 滕珂², 周仂², 刘凌云², 袁苏凡², 范希峰², 穆娜²

1. 北京农学院, 北京 102206;
2. 北京市农林科学院草业花卉与景观生态研究所, 北京 100097

通讯作者: 范希峰, E-mail: fanxifeng@baafs.net.cn;穆娜, E-mail: muna@baafs.net.cn
作者简介:史志福(2001-),男,汉族,河北邯郸人,硕士研究生,主要从事狼尾草育种研究,E-mail:3405966520@qq.com

Abstract

Abstract: To clarify the molecular characteristics of the WRKY gene family in highly cold-resistant Pennisetum alopecuroides and their functions in cold stress response， in this study we conducted systematic identification， phylogenetic analysis and cold stress-related expression pattern analysis of the WRKY gene family via combined genome-wide and transcriptome analyses. The results showed that a total of 104 PaWRKY genes with complete WRKY domains were identified， which were unevenly distributed on nine chromosomes. All the encoded proteins were hydrophilic， and most of them had poor in vitro stability and were susceptible to degradation. Based on phylogenetic analysis， the taxa were divided into seven subtribes， and Subtribe IIc comprises the most members， and ten core conserved motifs were predicted in total. Extensive homologous collinearity was found between this gene family and Oryza sativa subsp. japonica. The promoter regions of PaWRKY genes were enriched with numerous cis-acting elements responsive to abiotic stresses and phytohormones. Transcriptome data revealed that eight PaWRKY genes were differentially expressed under cold stress， including four upregulated and four downregulated genes， all of which harbored MeJA-responsive elements. This study clarified the molecular and evolutionary characteristics of the WRKY gene family in Pennisetum alopecuroides and screened out key cold stress-responsive genes， which laid a theoretical foundation for cold-tolerant molecular breeding of Pennisetum alopecuroides and evolutionary research of the WRKY gene family in Panicoideae.

Key words: Pennisetum alopecuroides, WRKY gene family, Cold stress, Differential expression

摘要： 为解析高抗寒性狼尾草（Pennisetum alopecuroides） WRKY基因家族的分子特征及其在冷胁迫响应中的作用，本研究采用全基因组与转录组联合分析，对 WRKY 基因家族进行系统鉴定、进化分析及冷胁迫表达模式研究。结果表明：共鉴定出104个含完整 WRKY 结构域的 PaWRKY 基因，这些基因不均匀分布于9条染色体；编码蛋白均为亲水蛋白，多数体外稳定性较弱，易降解。经系统进化分析，将其分为7个亚族，Ⅱc 亚族成员最多，共预测10个核心保守基序；该家族与粳稻存在广泛同源共线性关系。PaWRKY 基因启动子区富集大量逆境与激素响应顺式作用元件。转录组数据表明，8个 PaWRKY 基因在冷胁迫下出现差异表达，其中4个上调，4个下调，且均含有茉莉酸甲酯响应元件。本研究明确了狼尾草 WRKY 家族的分子与进化特征，筛选获得冷胁迫响应关键基因，为狼尾草抗寒分子育种及黍亚科 WRKY 家族进化研究提供理论依据。

关键词: 狼尾草, WRKY 基因家族, 冷胁迫, 差异表达

CLC Number:

S543.9

SHI Zhi-fu, WANG Xin, YUE Yue-sen, TENG Ke, ZHOU Le, LIU Ling-yun, YUAN Su-fan, FAN Xi-feng, MU Na. Genome-Wide Identification and Cold Stress Expression Analysis of the WRKY Gene Family in Pennisetum alopecuroides[J]. Acta Agrestia Sinica, 2026, 34(9): 3197-3210.

史志福, 王鑫, 岳跃森, 滕珂, 周仂, 刘凌云, 袁苏凡, 范希峰, 穆娜. 狼尾草WRKY基因家族全基因组鉴定与冷胁迫表达分析[J]. 草地学报, 2026, 34(9): 3197-3210.

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Genome-Wide Identification and Cold Stress Expression Analysis of the WRKY Gene Family in Pennisetum alopecuroides

狼尾草WRKY基因家族全基因组鉴定与冷胁迫表达分析

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