Effects of NaCl Stress on the Electric Conductivity and Fluorescence Parameters of Medicago ruthenica

doi:10.11733/j.issn.1007-0435.2013.05.012

Acta Agrestia Sinica ›› 2013, Vol. 21 ›› Issue (5): 913-920.DOI: 10.11733/j.issn.1007-0435.2013.05.012

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Effects of NaCl Stress on the Electric Conductivity and Fluorescence Parameters of Medicago ruthenica

YANG Yu-ping¹, CAI Li-yan¹, LI Zhi-yong¹, WANG Cheng-hai², SHI Wen-gui¹, LI Hong-yan¹, LIU Lei¹

1. Grassland Research Institute, Chinese Academy of Agricultural Sciences, Huhhot, Inner Mongolia 010010 China;
2. Grassland Supervision Station of Chayouhouqi, Wulanchabu, Inner Mongolia 010024, China

Received:2013-04-10 Revised:2013-06-07 Online:2013-10-15 Published:2013-10-30

NaCl胁迫对扁蓿豆叶片膜透性和叶绿素荧光特性的影响

杨玉平¹, 蔡丽艳¹, 李志勇¹, 王成海², 师文贵¹, 李鸿雁¹, 刘磊¹

1. 中国农业科学院草原研究所, 内蒙古呼和浩特 010010;
2. 乌兰察布市察右后旗草原监理所, 内蒙古乌兰察布 012400

通讯作者: 李志勇
作者简介:杨玉平(1958- ),男,内蒙古托克托人,副研究员,主要从事草原生态学研究,E-mail: yangyuping58@126.com
基金资助:
内蒙古自治区科技计划项目"优良牧草新品种土默特扁蓿豆标准化生产示范";农业部农作物种质资源保护与利用项目"多年生牧草更新复壮与利用"(NB2013-2130135-48);农业部牧草种质资源保护项目"牧草温带备份库的维护与备份繁殖入库"(2013-15)资助

Abstract

Abstract: Cell membrane permeability and chlorophyll fluorescence parameters were studied under the different NaCl treatments using three kinds of Medicago ruthenica seedlings in order to provide the theoretical basis of studying the salt tolerance mechanisms of Medicago ruthenica seedlings and breeding. Results showed that the cell membrane permeability, the minimal fluorescence (F₀), and non photochemical quenching coefficient (qN) increased with the increase of NaCl concentration, whereas the maximum fluorescence (F_m), maximum photochemical efficiency of PSⅡ (F_v/F_m), PSⅡ activity (F_v/F₀) and photochemical quenching (qP) decreased. Both PSⅡ acyclic photosynthetic electron transfer rate (ETR) and actual photochemical quantum yield (Y) were first increased then decreased. These results indicated that NaCl inhibited PSⅡ functionality and decreased PSⅡ center activity. The amplitude of chlorophyll fluorescence parameter was different with salt stress increasing among three kinds of Medicago ruthenica. The salt tolerance of Medicago ruthenica was comprehensive evaluated using the membership function of fuzzy mathematics. MR2 showed the highest tolerance to salt.

Key words: Medicago ruthenica, Salt-stress, Electric conductivity, Chlorophyll fluorescence

摘要： 以3份扁蓿豆(Medicago ruthenica)幼苗为试验材料,研究了不同NaCl浓度对其细胞膜透性和叶绿素荧光参数的影响,以期为选育扁蓿豆耐盐品种和揭示耐盐机制提供理论依据。结果表明:随着NaCl浓度的增加,3份扁蓿豆材料的细胞膜透性、初始荧光(F₀)、非光化学猝灭(qN)逐渐上升;最大荧光(F_m)、PSⅡ最大光化学效率(F_v/F_m)、潜在光化学活性(F_v/F₀)和光化学猝灭(qP)均呈下降趋势,而PSⅡ非循环光合电子传递速率(ETR)和实际光化学量子产量(Y)均先上升后下降;说明NaCl胁迫抑制了PSⅡ的功能,使PSⅡ反应中心活性下降。3份扁蓿豆的叶绿素荧光参数随盐胁迫增加变幅不同,用模糊数学的隶属函数法对3份扁蓿豆的耐盐性进行综合评价,MR2的耐盐性最强。

关键词: 扁蓿豆, 盐胁迫, 电导率, 叶绿素荧光参数

CLC Number:

Q945.78

YANG Yu-ping, CAI Li-yan, LI Zhi-yong, WANG Cheng-hai, SHI Wen-gui, LI Hong-yan, LIU Lei. Effects of NaCl Stress on the Electric Conductivity and Fluorescence Parameters of Medicago ruthenica[J]. Acta Agrestia Sinica, 2013, 21(5): 913-920.

杨玉平, 蔡丽艳, 李志勇, 王成海, 师文贵, 李鸿雁, 刘磊. NaCl胁迫对扁蓿豆叶片膜透性和叶绿素荧光特性的影响[J]. 草地学报, 2013, 21(5): 913-920.

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References

[1] 罗青红,李志军,伍维模,等. 胡杨、灰叶胡杨光合及叶绿素荧光特性的比较研究[J]. 西北植物学报,2006,26(5):983-988

[2] 郝建卿,吕娜,杨扬,等. 内蒙乌海胡杨异形叶水分及叶绿素荧光参数的比较[J]. 北京林业大学学报,2010,32(5):41-44

[3] 孙坤,李岩,冯汉青. NaCl胁迫对架豆叶绿素荧光参数的影响[J]. 西北师范大学学报:自然科学版,2011,47(5):74-77

[4] Colom M R, Vazzana C. Photosynthesis and PSⅡ functionality of drought-resistant and drought-sensitive weeping lovegrass plants[J]. Environmental and Experimental Botany,2003,49(2):135-144

[5] 张海波,崔继哲,曹甜,等. 大豆出苗期和苗期对盐胁迫的响应及耐盐指标评价[J]. 生态学报,2011,31(10):2805-2812

[6] 张恒,郑宝江,宋保华,等. 植物盐胁迫应答蛋白质组学分析[J]. 生态学报,2011,31(22):6936-6946

[7] 高奔,宋杰,刘金萍,等. 盐胁迫对不同生境盐地碱蓬光合及离子积累的影响[J]. 植物生态学报,2010,34(6):671-677

[8] Lawlor D W, Cornic G. Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants[J]. Plant Cell and Environment,2002,25(2):275-294

[9] 汪月霞,孙国荣,王建波,等. NaCl胁迫下星星草幼苗MDA含量与膜透性及叶绿素荧光参数之间的关系[J]. 生态学报,2006,26(1):122-129

[10] Belkhodja R, Morales F, Abadia A, et al. Chlorophyll fluorescence as a possible tool for salinity tolerance screening in Barley (Hordenm vulgare L.)[J]. Plant Physiology,1994,104(2):667-673

[11] 吴雪霞,查丁石. NaCl胁迫对茄子幼苗叶片叶绿素荧光参数和能量分配的影响[J]. 华北农学报,2009,24(6):83-87

[12] 石凤翎,郭晓霞,李红. 扁蓿豆抗旱形态解剖结构观察与分析[J]. 干旱地区农业研究,2005,23(2):115-118

[13] 蔡丽艳,李志勇,孙启忠,等.扁蓿豆萌发对干旱胁迫的响应及抗旱性评价[J]. 草业科学,2012,29(10):1553-1559

[14] 李小安. 低温胁迫对青藏扁蓿豆与和田苜蓿种子游离脯氨酸和可溶性糖含量的影响[J]. 青海大学学报:自然科学版,2011,29(4):10-14

[15] 李鸿雁,李志勇,赵成贵,等. 扁蓿豆叶片解剖结构特征与生态因子相关性分析[J]. 草地学报,2012,20(2):236-243

[16] 郝建辉,石凤翎. 不同扁蓿豆材料抗旱性比较研究[J]. 中国草地学报,2006,28(3):39-43

[17] 韩海波,师文贵,李志勇,等. 扁蓿豆的抗性研究进展[J]. 草业科学,2011,28(4):631-635

[18] 史万光,王照兰,杜建材,等. 扁蓿豆不同品系种子发芽期耐盐性鉴定[J]. 中国草地学报,2008,30(1):40-44

[19] Guan B, Zho u D, Zhang H, et al. Germination responses of Medicago ruthenica seeds to salinity, alkalinity, and temperature[J]. Journal of Arid Environments,2009,73(1):135-138

[20] 王晓栋. 10份豆科牧草种质材料耐盐性研究[D]. 呼和浩特:内蒙古农业大学,2008

[21] Premachandra G S, Saneoka H, Fujita K, et al. Leaf water relations, osmotic adjustment, cell membrane stability, epicuticular wax load and growth as affected by increasing water deficits in Sorghum[J]. Journal of Experimental Botany,1992,43(12):1569-1576

[22] Hu Y B, Sun G Y, Wang X C. Induction characteristics and response of photosynthetic quantum conversion to changes in irradiance in mulberry plants[J]. Journal of Plant Physiology,2007,164(8):959-968

[23] 陈志刚,谢宗强,郑海水. 不同地理种源西南桦苗木的耐热性研究[J]. 生态学报,2003,23(11):2327-2332

[24] 孙群,胡景江. 植物生理学研究技术[M]. 杨凌:西北农林科技大学出版社,2005:67

[25] 颉建明,郁继华,颉敏华. 青花菜叶片PSⅡ光化学效率和光合对强光高温的响应[J]. 兰州大学学报:自然科学版,2008,44 (1):51-55

[26] 张守仁. 叶绿素荧光动力学参数的意义及讨论[J]. 植物学通报,1999,16(4):444-448

[27] 张德罡. 盐胁迫对五个早熟禾草坪草品种苗期细胞膜伤害性的研究[J]. 甘肃农业大学学报,1998,33(1):38-41

[28] 张俊莲,陈勇胜,武季玲,等. 向日葵对盐逆境伤害的生理反应及耐盐性研究[J]. 中国油料作物学报,2003,25(1):45-49

[29] 过晓明,张楠,马代夫,等. 盐胁迫对5种甘薯幼苗叶片叶绿素含量和细胞膜透性的影响[J]. 江苏农业科学,2010(3):93-94

[30] 金微微,王炎,张会慧,等. 茉莉酸甲酯对干旱及复水条件下烤烟幼苗叶绿素荧光特性的影响[J]. 应用生态学报,2011,22(12):3157-3162

[31] Lu C, Qiu N, Lu Q, et al. Does salt stress lead to increased susceptibility of photosystem Ⅱ to photoinhibition and changes in photosynthetic pigment composition in halophyte Suaeda salsa grown outdoors?[J]. Plant Science,2002,163(5):1063-1068

[32] Sunyo J. Variation in antioxidant metabolism of young and mature leaves of Arabidopsis thaliana subject ed to drought[J]. Plant Science,2004,166(2):459-466

[33] Yang W, Liu S, Feng F Y, et al. Effects of phosphate deficiency on the lipid composition in cucumber thylakoid membranes and PSⅡ particles[J]. Plant Science,2004,166(6):1575-1579

Effects of NaCl Stress on the Electric Conductivity and Fluorescence Parameters of Medicago ruthenica

NaCl胁迫对扁蓿豆叶片膜透性和叶绿素荧光特性的影响

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