Acta Agrestia Sinica ›› 2013, Vol. 21 ›› Issue (5): 913-920.DOI: 10.11733/j.issn.1007-0435.2013.05.012

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Effects of NaCl Stress on the Electric Conductivity and Fluorescence Parameters of Medicago ruthenica

YANG Yu-ping1, CAI Li-yan1, LI Zhi-yong1, WANG Cheng-hai2, SHI Wen-gui1, LI Hong-yan1, LIU Lei1   

  1. 1. Grassland Research Institute, Chinese Academy of Agricultural Sciences, Huhhot, Inner Mongolia 010010 China;
    2. Grassland Supervision Station of Chayouhouqi, Wulanchabu, Inner Mongolia 010024, China
  • Received:2013-04-10 Revised:2013-06-07 Online:2013-10-15 Published:2013-10-30

NaCl胁迫对扁蓿豆叶片膜透性和叶绿素荧光特性的影响

杨玉平1, 蔡丽艳1, 李志勇1, 王成海2, 师文贵1, 李鸿雁1, 刘磊1   

  1. 1. 中国农业科学院草原研究所, 内蒙古 呼和浩特 010010;
    2. 乌兰察布市察右后旗草原监理所, 内蒙古 乌兰察布 012400
  • 通讯作者: 李志勇
  • 作者简介:杨玉平(1958- ),男,内蒙古托克托人,副研究员,主要从事草原生态学研究,E-mail: yangyuping58@126.com
  • 基金资助:

    内蒙古自治区科技计划项目"优良牧草新品种土默特扁蓿豆标准化生产示范";农业部农作物种质资源保护与利用项目"多年生牧草更新复壮与利用"(NB2013-2130135-48);农业部牧草种质资源保护项目"牧草温带备份库的维护与备份繁殖入库"(2013-15)资助

Abstract: Cell membrane permeability and chlorophyll fluorescence parameters were studied under the different NaCl treatments using three kinds of Medicago ruthenica seedlings in order to provide the theoretical basis of studying the salt tolerance mechanisms of Medicago ruthenica seedlings and breeding. Results showed that the cell membrane permeability, the minimal fluorescence (F0), and non photochemical quenching coefficient (qN) increased with the increase of NaCl concentration, whereas the maximum fluorescence (Fm), maximum photochemical efficiency of PSⅡ (Fv/Fm), PSⅡ activity (Fv/F0) and photochemical quenching (qP) decreased. Both PSⅡ acyclic photosynthetic electron transfer rate (ETR) and actual photochemical quantum yield (Y) were first increased then decreased. These results indicated that NaCl inhibited PSⅡ functionality and decreased PSⅡ center activity. The amplitude of chlorophyll fluorescence parameter was different with salt stress increasing among three kinds of Medicago ruthenica. The salt tolerance of Medicago ruthenica was comprehensive evaluated using the membership function of fuzzy mathematics. MR2 showed the highest tolerance to salt.

Key words: Medicago ruthenica, Salt-stress, Electric conductivity, Chlorophyll fluorescence

摘要: 以3份扁蓿豆(Medicago ruthenica)幼苗为试验材料,研究了不同NaCl浓度对其细胞膜透性和叶绿素荧光参数的影响,以期为选育扁蓿豆耐盐品种和揭示耐盐机制提供理论依据。结果表明:随着NaCl浓度的增加,3份扁蓿豆材料的细胞膜透性、初始荧光(F0)、非光化学猝灭(qN)逐渐上升;最大荧光(Fm)、PSⅡ最大光化学效率(Fv/Fm)、潜在光化学活性(Fv/F0)和光化学猝灭(qP)均呈下降趋势,而PSⅡ非循环光合电子传递速率(ETR)和实际光化学量子产量(Y)均先上升后下降;说明NaCl胁迫抑制了PSⅡ的功能,使PSⅡ反应中心活性下降。3份扁蓿豆的叶绿素荧光参数随盐胁迫增加变幅不同,用模糊数学的隶属函数法对3份扁蓿豆的耐盐性进行综合评价,MR2的耐盐性最强。

关键词: 扁蓿豆, 盐胁迫, 电导率, 叶绿素荧光参数

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