Chloroplast Photosynthesis during Seed Germination of Smooth Bromegrass and Oat

doi:10.11733/j.issn.1007-0435.2023.10.018

Acta Agrestia Sinica ›› 2023, Vol. 31 ›› Issue (10): 3058-3066.DOI: 10.11733/j.issn.1007-0435.2023.10.018

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Chloroplast Photosynthesis during Seed Germination of Smooth Bromegrass and Oat

TIAN Pei-xin, SONG Rui, LI Chao-ran, NI Hao-ran, WANG Run, LIU Ping, ZHANG Han, MAO Pei-sheng, JIA Shan-gang

School of Grassland Science and Technology, China Agricultural University, Beijing 100193, China

Received:2023-03-31 Revised:2023-05-20 Online:2023-10-15 Published:2023-11-02

无芒雀麦和燕麦种子萌发过程中叶绿体光合变化

田沛鑫, 宋瑞, 李超然, 倪浩然, 王润, 刘萍, 张涵, 毛培胜, 贾善刚

中国农业大学草业科学与技术学院, 北京 100193

通讯作者: 贾善刚,E-mail:shangang.jia@cau.edu.cn
作者简介:田沛鑫(1999-),女,汉族,山西忻州人,硕士研究生,主要从事育种与种子科学研究,E-mail:peixin.tian@cau.edu.cn;宋瑞(1995-),女,汉族,宁夏吴忠人,硕士研究生,主要从事育种与种子科学研究,E-mail:ruisong2021@cau.edu.cn
基金资助:
“国家重点研发计划”（2022YFD1300804）；“现代农业产业技术体系”（CARS-34）；“四川省省院省校合作重点研发项目”（2023YFSY0012）资助

Abstract

Abstract: In this study,smooth bromegrass and oat seeds were used as experimental materials. The culture dishes wrapped in tinfoil were used to simulate the darkness for 5~6 days,and then the tinfoil was unwrapped to simulate the germination. The spectral phenotype,photosynthetic pigment content and chlorophyll photosynthetic fluorescence parameters of the plumule of two species were analyzed during this period. The results showed that,the phenotypes of the plumule of smooth bromegrass and oat were significantly changed after being exposed to light,and the contents of photosynthetic pigments showed an overall increasing trend:Chlorophyll a and chlorophyll b accumulated slowly for 1 h (E1h) of light exposure in the first day,and increased rapidly after 1 d (E1d) of light exposure. Carotenoid content decreased in E1d and then increased rapidly. The chlorophyll fluorescence parameters showed an overall trend of increase,among which,in smooth bromegrass the maximum optical efficiency (F_v/F_m) and effective photochemical quantum yield (F_v'/F_m') of PSII increased in E1h-E1d and E2.5h-E1d,respectively;and tended to be stable after E1d. In contrast,F_v/F_m and F_v'/F_m' of oat increased gradually during E1h-E1d. In conclusion,the important stage of chloroplast formation is from 1 hour to 1 day (E1h-E1d) after the smooth bromegrass and oat seeds were exposed to light from the embedded darkness,and the chloroplast function was basically developed and matured after E1d. The results in this study will provide support for further study for the chloroplast biogenesis during seed germination of gramineous forages,and have certain significance for the analysis of the dynamic changes during seed germination.

Key words: Seed germination, Chloroplast biogenesis, Photosynthetic pigment, Chlorophyll fluorescence parameters, Multispectral imaging

摘要： 本研究以无芒雀麦（Bromus inermis Leyss.）和燕麦（Avena sativa L.）种子为试验材料，利用锡纸包裹培养皿模拟黑暗5~6天，揭开锡纸模拟种子萌发，分析胚芽光谱表型、光合色素含量及叶绿素光合荧光参数。结果表明，无芒雀麦和燕麦照光后胚芽表型发生显著变化，无芒雀麦与燕麦胚芽光合色素含量整体呈上升趋势：叶绿素a和叶绿素b揭开锡纸第1天光照第1 h（E1 h）后缓慢积累，光照1天（E1 d）后迅速增加；类胡萝卜素在E1 d先下降后迅速升高。叶绿素荧光参数整体上升，无芒雀麦PSII最大光学效率（F_v/F_m）在E1h—E1d迅速上升，PSII有效光化学量子产量（F_v'/F_m'）在E2.5h—E1d上升，燕麦F_v/F_m和F_v'/F_m'在E1h—E1d上升。综上，无芒雀麦和燕麦种子在揭开锡纸后的E1h—E1d为叶绿体发生重要阶段，在E1 d后叶绿体结构基本发育成熟。本试验结果为深入研究禾本科牧草种子萌发叶绿体生物发生提供支持，对解析叶绿体生物发生动态变化规律具有重要意义。

关键词: 种子萌发, 叶绿体发育, 光合色素, 叶绿素荧光参数, 多光谱成像

CLC Number:

S330.2

TIAN Pei-xin, SONG Rui, LI Chao-ran, NI Hao-ran, WANG Run, LIU Ping, ZHANG Han, MAO Pei-sheng, JIA Shan-gang. Chloroplast Photosynthesis during Seed Germination of Smooth Bromegrass and Oat[J]. Acta Agrestia Sinica, 2023, 31(10): 3058-3066.

田沛鑫, 宋瑞, 李超然, 倪浩然, 王润, 刘萍, 张涵, 毛培胜, 贾善刚. 无芒雀麦和燕麦种子萌发过程中叶绿体光合变化[J]. 草地学报, 2023, 31(10): 3058-3066.

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References

[1] 赖帅彬,潘新雅,简春霞,等. 转苜蓿MsOr基因烟草光合-光响应和光合-CO₂响应曲线特征研究[J]. 草地学报,2020,28(1):20-30
[2] 谭炎宁,孙学武,袁定阳,等. 水稻单叶独立转绿型黄化突变体grc2的鉴定与基因精细定位[J]. 作物学报,2015,41(6):831-837
[3] 付佳琳,蔡江平,刘贺永,等. 内蒙古草甸草原植物叶片光合色素对极端干旱的响应[J]. 中国草地学报,2022,44(4):40-47
[4] 童立豪,吴翔宇,黄良夫,等. 光照强度与琼枝藻生长、光合色素和颜色值的相关性分析[J]. 南方水产科学,2021,17(5):79-85
[5] EL-HENDAWY S,AL-SUHAIBANI N,HASSAN W,et al. Hyperspectral reflectance sensing to assess the growth and photosynthetic properties of wheat cultivars exposed to different irrigation rates in an irrigated arid region[J]. PLoS One,2017,12(8):e0183262
[6] 许中秋,隋德宗,谢寅峰,等. 两个乌桕新品种苗木光合特性比较[J]. 南京林业大学学报(自然科学版),2021,45(1):93-100
[7] 李晓,冯伟,曾晓春. 叶绿素荧光分析技术及应用进展[J]. 西北植物学报,2006(10):2186-2196
[8] 徐德聪,吕芳德,刘小阳,等. 叶绿素荧光测定技术的研究[J]. 安徽农业科学,2007(35):11335-11337
[9] 王雪萌,何欣,张涵,等. 基于多光谱成像技术快速无损检测紫花苜蓿人工老化种子[J]. 草业学报,2022,31(7):197-208
[10] 岳佳铭,丛晓翔,李曼莉. 多光谱成像技术在种子质量检测研究中的应用[J]. 种子,2021,40(10):129-135
[11] 王众司,贾亚萍,张瑾,等. 多光谱成像技术在植物学研究中的应用[J]. 植物学报,2021,56(4):500-508
[12] 孙光明,杨凯盛,张传清,等. 基于多光谱成像技术的大麦赤霉病识别[J]. 农业工程学报,2009,25(S2):204-207
[13] HU X W,YANG L J,ZHANG Z X,et al. Differentiation of alfalfa and sweet clover seeds via multispectral imaging[J]. Seed Science and Technology,2020,48(1):83-99
[14] WANG X M,ZHANG H,SONG R,et al. Non-destructive identification of naturally aged alfalfa seeds via multispectral imaging analysis[J]. Sensors,2021,21(17):5804
[15] YANG L,ZHANG Z,HU X. Cultivar discrimination of single alfalfa (Medicago sativa L.) seed via multispectral imaging combined with multivariate analysis[J]. Sensors,2020,20(22):6575
[16] PAN W J,WANG X,DENG Y R,et al. Nondestructive and intuitive determination of circadian chlorophyll rhythms in soybean leaves using multispectral imaging[J]. Scientific Reports,2015,5(1):1-13
[17] EBERHARD S,FINAZZI G,WOLLMAN F. The dynamics of photosynthesis[J]. Annual Review of Genetics,2008(42):463-515
[18] 汪爱霞,马彦麟,齐广平,等. 无芒雀麦与苜蓿混播草地生产力提升的水氮调控模式[J]. 水土保持学报,2022,36(2):322-330
[19] 王玉祥,杜雨,陈映霞,等. 盐胁迫对无芒雀麦种子萌发及苗期生理指标的影响[J]. 干旱区资源与环境,2022,36(5):139-145
[20] 王运琦,崔晓静,吴增禄,等. 三种牧草消化率及营养价值比较试验[J]. 山西农业科学,1991(10):13-14
[21] 宋家兴,李倩,罗金,等. 37份新疆无芒雀麦萌发期耐盐性评价[J]. 种子,2020,4(39):85-90
[22] 杨志敏,李峰,刘建成,等. 冀西北农牧交错区24个燕麦品种的生产性能和饲用价值比较[J]. 河北农业科学,2022,26(1):54-60
[23] 魏晓丽,徐成体,段娜宁,等. 高寒地区8种燕麦生产性能比较研究[J]. 青海畜牧兽医杂志,2022,52(1):14-19
[24] 吴志. 饲用燕麦的营养价值及利用探析[J]. 现代农业科技,2020(12):223-225
[25] 熊璇,于晓英,魏湘萍,等. 遮荫对重瓣大花萱草光合色素含量及生理特性的影响[J]. 湖南农业科学,2009(1):29-32
[26] 童方平,方伟,马履一,等. 水分胁迫下湿地松优良半同胞家系光合色素的响应[J]. 中国农学通报,2006(11):97-102
[27] 包维楷,冷俐. 苔藓植物光合色素含量测定方法-以暖地大叶藓为例[J]. 应用与环境生物学报,2005(2):235-237
[28] 吴菲菲,曹永仁,滕延福. 玉米叶绿素荧光参数的测定与分析[J]. 农业装备技术,2022,48(1):33-35
[29] 孙化雨,娄永峰,赵韩生,等. 开花麻竹叶绿素荧光参数测定[J]. 世界竹藤通讯,2014,12(3):12-16
[30] 丛晓翔. 利用多光谱成像技术测定多年生黑麦草种子活力的研究[D]. 北京:中国农业大学,2020:49-57
[31] ELMASRY G,MANDOUR N,WAGNER M H,et al. Utilization of computer vision and multispectral imaging techniques for classification of cowpea (Vigna unguiculata) seeds[J]. Plant Methods,2019,15(1):24
[32] 吴甘霖,羊礼敏,段仁燕,等. 遮荫对大别山五针松幼苗光合色素和光合作用的影响[J]. 安庆师范学院学报(自然科学版),2016,22(3):106-109
[33] 金雅琴,张丽娟,李冬林,等. 干旱胁迫对乌桕幼苗光合色素及光合性能的影响[J]. 江西农业大学学报,2011,33(4):731-737
[34] 李伟才,孙光明,弓德强,等. 不同砧穗组合对AP番荔枝光合作用和光合色素的影响[J]. 安徽农业科学,2008(14):5747-5749
[35] 王峰,闫家榕,陈雪玉,等. 光调控植物叶绿素生物合成的研究进展[J]. 园艺学报,2019,46(5):975-994
[36] 刘柿良,马明东,潘远智,等. 不同光强对两种桤木幼苗光合特性和抗氧化系统的影响[J]. 植物生态学报,2012,36(10):1062-1074
[37] 敏张,朱教君,闫巧玲. 光对种子萌发的影响机理研究进展[J]. 植物生态学报,2012,36(8):899-908
[38] 周福利. 夜温对辣椒幼苗叶片光合色素的影响[J]. 甘肃农业科技,2020(9):22-27
[39] ZULUAGA M,GUEGUEN V,PAVON-DJAVID G,et al. Carotenoids from microalgae to block oxidative stress[J]. BioImpacts,2017,7(1):1-3
[40] 王军,赵桂琴,柴继宽,等. 大麦黄矮病毒侵染对燕麦光合及叶绿素荧光参数的影响[J]. 草地学报,2020,28(4):923-931
[41] 刘盈盈,张珍明,康超,等. 不同种源青钱柳幼苗生长及生理生化特性的比较[J]. 北方园艺,2016(15):57-61
[42] 孙红英,曹光球,辛全伟,等. 香樟8个无性系叶绿素荧光特征比较[J]. 福建林学院学报,2010,30(4):309-313

Chloroplast Photosynthesis during Seed Germination of Smooth Bromegrass and Oat

无芒雀麦和燕麦种子萌发过程中叶绿体光合变化

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