›› 2013, Vol. 21 ›› Issue (6): 1144-1147.DOI: 10.11733/j.issn.1007-0435.2013.06.017

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Study on the Salt Tolerance of Transgenic Tall Fescue

MA Dong-mei1, JIN Feng-xia1, XU Xing2, LI Hui-wen2, WEI Rui2, GUO Ling-na2   

  1. 1. Agricultural College, Ningxia University, Yinchuan, Ningxia 750021, China;
    2. College of Life Science, Ningxia University, Yinchuan, Ningxia 750021, China
  • Received:2013-05-07 Revised:2013-09-26 Online:2013-12-15 Published:2013-11-27

高羊茅耐盐转基因抗性苗的耐盐性研究

麻冬梅1, 金凤霞1, 许兴2, 李会文2, 魏睿2, 郭伶娜2   

  1. 1. 宁夏大学农学院, 宁夏 银川 750021;
    2. 宁夏大学生命科学学院, 宁夏 银川 750021
  • 作者简介:麻冬梅(1978-),女,宁夏银川人,博士,讲师,主要从事牧草育种与植物抗逆分子生物学研究,E-mail:mdm319@tom.com
  • 基金资助:
    国家973项目“鉴定由调控网络重要节点构成的调控单元,并进行耐盐碱作物分子育种设计课题”(2012CB114204);“十一五”国家科技支撑计划资助项目“荒漠草原区农牧复合生态系统构建与可持续利用技术集成试验与示范”(2011BAC07B03);宁夏农发办项目(NTKJ-2013-09(1))资助

Abstract: Transgenic ‘Arid 3’ plants (Festuca arundinacea) under fully controlled conditions treated with the different concentrations of NaCl for 14 days were used as test materials in this study. The physiological indexes related to salt tolerance, such as proline (Pro) and malondialdehyde (MDA) contents, electrical conductivity rate, superoxide dismutase (SOD) and catalase (POD) activities were measured. Results showed that Pro contents, SOD and POD activities of transgenic seedling were significant higher than that of control(P<0.05), whereas the relative conductivity and MDA content of transgenic seedling were lower than that of control when NaCl concentration was above 1.5%(P<0.05). These results suggest that the salt tolerance of the transgenic plant is obviously enhanced.

Key words: Transgene, Tall fescue, Salt tolerance

摘要: 以转基因高羊茅爱瑞三号(Festuca arundinacea ‘Arid 3’)植株为材料,采用不同梯度的NaCl进行盐胁迫处理14 d后,通过对其脯氨酸(Pro)含量、丙二醛(MDA)含量、电导率及超氧化物歧化酶(SOD)活性和过氧化物酶(POD)活性等抗旱耐盐性生理生化指标的测定,进行了耐盐性鉴定研究。结果表明:当NaCl浓度大于1.5%,在同一盐浓度下转基因植株的Pro含量、SOD和POD活性均显著高于对照植株(P<0.05),而相对电导率和MDA含量低于对照植株(P<0.05)。这表明转基因植株的耐盐性得到了显著增强。

关键词: 转基因, 高羊茅, 耐盐性

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