Polyamines Regulate Drought Tolerance in Kentucky Bluegrass Cultivar 'Qinghai’

doi:10.11733/j.issn.1007-0435.2024.04.025

Acta Agrestia Sinica ›› 2024, Vol. 32 ›› Issue (4): 1210-1216.DOI: 10.11733/j.issn.1007-0435.2024.04.025

Polyamines Regulate Drought Tolerance in Kentucky Bluegrass Cultivar 'Qinghai’

LI Yu-hui, LIU Rui, LUO Yu-rong, LU Shao-yun

College of Life Science, South China Agricultural University, Guangzhou, Guangdong Province 510642, China

Received:2023-08-29 Revised:2023-10-18 Published:2024-05-07

多胺调控青海草地早熟禾抗旱性

李钰辉, 刘瑞, 罗玉容, 卢少云

华南农业大学生命科学学院, 广东广州 510642

通讯作者: 卢少云,E-mail:turflab@scau.edu.cn
作者简介:李钰辉(1998-)，男，汉族，广东湛江人，硕士研究生，主要从事草坪草的抗逆研究，E-mail：20203164108@scau.edu.cn
基金资助:
国家自然科学基金项目(U21A20241)资助

Abstract

Abstract: The native Kentucky bluegrass cultivar 'Qinghai’ (Poa pratensis L. cv. Qinghai),known for its high cold tolerance,has been extensively used in the restoration of degraded alpine grassland in the source of Three rivers in Qinghai Province. However,its drought tolerance remains unexplored. This study investigated the physiological responses of the 'Qinghai’ cultivar to drought using polyethylene glycol (PEG) as a simulated drought treatment. We also examined the effects of exogenous putrescine and spermidine treatments on improving the drought tolerance. The results showed that proline,putrescine,spermidine,and spermine levels were continuously increased after treatment with PEG. This increase was accompanied by elevated transcript levels of genes involved in polyamine biosynthesis,including ADC1,ADC2-1,SAMDC2, and SPDS1. Proline levels showed a positive relationship with putrescine,spermidine,and spermine levels. In addition,pretreatment with exogenous putrescine and spermidine led to increased drought resistance and proline levels in 'Qinghai’ under drought stress. It is suggested that polyamines regulate drought tolerance in 'Qinghai’ through promoted proline accumulation under drought conditions.

Key words: Poa pratensis L. cv. Qinghai, Polyamines, Proline, Drought tolerance

摘要： 青海草地早熟禾(Poa pratensis L.cv.Qinghai)具有极强的耐寒性，已广泛应用于青海三江源退化高寒草地的修复，但对其抗旱性研究较少。本研究利用聚乙二醇(Polyethylene glycol，PEG)模拟干旱处理青海草地早熟禾，测定其响应干旱的生理变化，并检测外源腐胺、亚精胺处理提高青海草地早熟禾抗旱性的效应。结果显示，PEG处理后，青海草地早熟禾脯氨酸、腐胺、亚精胺和精胺含量不断升高，脯氨酸含量的变化与腐胺、亚精胺和精胺含量正相关；多胺合成关键酶基因ADC1，ADC2-1，SAMDC2，SPDS1基因的表达量也提高。外源腐胺与亚精胺预处理能够提高青海草地早熟禾抗旱性和脯氨酸含量，本文研究结果表明，多胺通过调控干旱条件下脯氨酸的积累提高青海草地早熟禾抗旱性。

关键词: 青海草地早熟禾, 多胺, 脯氨酸, 抗旱性

CLC Number:

Q945

LI Yu-hui, LIU Rui, LUO Yu-rong, LU Shao-yun. Polyamines Regulate Drought Tolerance in Kentucky Bluegrass Cultivar 'Qinghai’[J]. Acta Agrestia Sinica, 2024, 32(4): 1210-1216.

李钰辉, 刘瑞, 罗玉容, 卢少云. 多胺调控青海草地早熟禾抗旱性[J]. 草地学报, 2024, 32(4): 1210-1216.

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Polyamines Regulate Drought Tolerance in Kentucky Bluegrass Cultivar 'Qinghai’

多胺调控青海草地早熟禾抗旱性

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