Effects of Plateau Pika Disturbance on Soil Archaea Community Characteristics in Alpine Meadow

doi:10.11733/j.issn.1007-0435.2025.12.012

Acta Agrestia Sinica ›› 2025, Vol. 33 ›› Issue (12): 3994-4003.DOI: 10.11733/j.issn.1007-0435.2025.12.012

• 2025-12-28 • Previous Articles

Effects of Plateau Pika Disturbance on Soil Archaea Community Characteristics in Alpine Meadow

WANG Si-yu^1,2,3,4, LI Lin^1,2,3,4, CHEN Ke-long^2,3,4, ZHANG Ni^2,3,4, SUN Jian-qing⁵, SHI Qian-qian⁵

1. College of Life Sciences, Qinghai Normal University, Xining, Qinghai Province 810008, China;
2. Qinghai Province Key Laboratory of Physical Geography and Environmental Process, College of Geographical Science, Qinghai Normal University, Xining, Qinghai Province 810008, China;
3. Key Laboratory of Tibetan Plateau Land Surface Processes and Ecological Conservation (Ministry of Education), Qinghai Normal University, Xining, Qinghai Province 810008, China;
4. National Positioning Observation and Research Station of Qinghai Lake Wetland Ecosystem in Qinghai, National Forestry and Grassland Administration, Haibei, Qinghai Province 812200, China;
5. Qinghai Lake National Nature Reserve Administration, Gangcha, Qinghai Province 812300, China

Received:2024-12-06 Revised:2025-06-20 Published:2025-12-01

高原鼠兔干扰对高寒草甸土壤古菌群落特征的影响

王丝瑜^1,2,3,4, 李琳^1,2,3,4, 陈克龙^2,3,4, 章妮^2,3,4, 孙建青⁵, 史倩倩⁵

1. 青海师范大学生命科学学院, 青海西宁 810008;
2. 青海师范大学地理科学学院青海省自然地理与环境过程重点实验室, 青海西宁 810008;
3. 青海师范大学青藏高原地表过程与生态保护教育部重点实验室, 青海西宁 810008;
4. 国家林业和草原局青海湖湿地生态系统国家定位观测研究站, 青海海北 812200;
5. 青海湖国家级自然保护区管理局, 青海刚察 812300

通讯作者: 陈克龙，E-mail：ckl7813@163.com；章妮，E-mail：1581146264@qq.com
作者简介:王丝瑜（2000-），女，汉族，陕西咸阳人，硕士研究生，主要从事湿地生态学研究，E-mail：2637286473@qq.com；
基金资助:
国家自然科学基金（42461018）；青海省科技计划（2023-ZJ-905T）资助

Abstract

Abstract: As a unique mammal in the alpine meadow ecosystem of the Qinghai-Tibet Plateau， the disturbance caused by plateau pikas （Ochotona curzoniae） may accelerate alpine meadow degradation The degradation of the alpine meadow has a significant affects on the soil archaeal communities. In this study， we investigated the characteristics of soil archaeal communities under pika disturbance by analyzing surface soil （0-10 cm，ST₁） and subsurface soil （10-20 cm，ST₂） through high-throughput sequencing of archaeal 16S rRNA. The results revealed that Thaumarchaeota remained the dominant archaeal phylum across all treatments， with Candidatus Nitrososphaera as the predominant genus. Compared to natural conditions， pika disturbance increased archaeal diversity in surface soil but decreased it in subsurface soil. LEfSe analysis demonstrated that pika activity significantly enhanced the abundance of three nitrogen-cycling taxa in subsurface soil： Candidatus Nitrososphaera， Marine Group II， and Thermoplasmata. In conclusion， plateau pika activities alter the structure of soil archaeal communities in alpine meadows， and the impact on the surface soil was more obvious.

Key words: High-throughput sequencing, Tibetan Plateau, Thaumarchaeota, Candidatus Nitrososphaera

摘要： 高原鼠兔作为青藏高原高寒草甸生态系统中的特有哺乳动物，其干扰可能加速高寒草甸退化，而高寒草甸退化对土壤古菌群落有显著影响。本研究以高原鼠兔干扰下的表层土壤（0~10 cm，ST₁）与亚表层土壤（10~20 cm，ST₂）作为研究对象，基于古菌16S rRNA进行高通量测序，对高原鼠兔干扰下的土壤古菌群落特征变化进行研究。结果表明：不同处理下土壤古菌的优势菌门均为奇古菌门（Thaumarchaeota），优势菌属均为亚硝化球菌属（Candidatus Nitrososphaera）；与自然状态相比，高原鼠兔干扰下土壤古菌群落的表层土壤多样性上升，亚表层土壤多样性下降；LEfSe分析发现高原鼠兔干扰增加亚表层土壤中亚硝化球菌属、海洋异养古菌类群（Marine Group II）及热原体纲（Thermoplasmata）3个与氮循环相关菌群的相对丰度。总而言之，高原鼠兔活动影响了高寒草甸土壤古菌的群落结构，且对表层土壤的影响较明显。

关键词: 高通量测序, 青藏高原, 奇古菌门, 亚硝化球菌属

CLC Number:

S812.2

WANG Si-yu, LI Lin, CHEN Ke-long, ZHANG Ni, SUN Jian-qing, SHI Qian-qian. Effects of Plateau Pika Disturbance on Soil Archaea Community Characteristics in Alpine Meadow[J]. Acta Agrestia Sinica, 2025, 33(12): 3994-4003.

王丝瑜, 李琳, 陈克龙, 章妮, 孙建青, 史倩倩. 高原鼠兔干扰对高寒草甸土壤古菌群落特征的影响[J]. 草地学报, 2025, 33(12): 3994-4003.

References

[1] TAN K，CIAIS P，PIAO S L，et al. Application of the ORCHIDEE global vegetation model to evaluate biomass and soil carbon stocks of Qinghai-Tibetan grasslands[J]. Global Biogeochemical Cycles，2010，24（1）：GB1013
[2] CHEN N，ZHANG Y J，ZHU J T，et al. Study on the nonlinear response mechanism of community productivity and species diversity in Alpine meadow degradation[J]. Chinese Journal of Plant Ecology，2018，42（1）：50-65 陈宁，张扬建，朱军涛，等. 高寒草甸退化过程中群落生产力和物种多样性的非线性响应机制研究[J]. 植物生态学报，2018，42（1）：50-65
[3] ZHANG Q，FAN K K，SINGH V P，et al. Is Himalayan-Tibetan Plateau “drying”？ Historical estimations and future trends of surface soil moisture[J]. Science of the Total Environment，2019，658：374-384
[4] WANG Z Q，ZHANG Y Z，YANG Y，et al. Quantitative assess the driving forces on the grassland degradation in the Qinghai-Tibet Plateau，in China[J]. Ecological Informatics，2016，33：32-44
[5] PENG F，XUE X，LI C Y，et al. Plant community of alpine steppe shows stronger association with soil properties than alpine meadow alongside degradation[J]. Science of the Total Environment，2020，733：139048
[6] PAN H，LIU H Y，LIU Y W，et al.Understanding the relationships between grazing intensity and the distribution of nitrifying communities in grassland soils[J]. Science of The Total Environment，2018，634：1157-1164
[7] LI H Y，QIU Y Z，YAO T，et al. Nutrients available in the soil regulate the changes of soil microbial community alongside degradation of alpine meadows in the northeast of the Qinghai-Tibet Plateau[J]. Science of the Total Environment，2021，792：148363
[8] SUN J J，WANG P B，WANG H B，et al. Changes in plant communities，soil characteristics，and microbial communities in alpine meadows degraded to different degrees by pika on the Qinghai-Tibetan Plateau[J]. Global Ecology and Conservation，2021，27：e01621
[9] WU Y R，YANG W Q，LI Q Y，et al. Microbial community response to alpine meadow degradation and its impact on soil nutrient cycling[J]. Agronomy，2024，14（1）：195
[10] ARTHUR A D，PECH R P，DAVEY C，et al. Livestock grazing，plateau pikas and the conservation of avian biodiversity on the Tibetan Plateau[J]. Biological Conservation，2008，141（8）：1972-1981
[11] WESCHE K，NADROWSKI K，RETZER V. Habitat engineering under dry conditions： the impact of pikas （Ochotona pallasi） on vegetation and site conditions in southern Mongolian steppes[J]. Journal of Vegetation Science，2007，18（5）：665
[12] CHEN J J，YI S H，QIN Y. The contribution of plateau pika disturbance and erosion on patchy alpine grassland soil on the Qinghai-Tibetan Plateau： implications for grassland restoration[J]. Geoderma，2017，297：1-9
[13] BADINGQUIYING，SMITH A T，HARRIS R B. Summer habitat use of plateau pikas （Ochotona curzoniae） in response to winter livestock grazing in the alpine steppe Qinghai-Tibetan Plateau[J]. Arctic，Antarctic，and Alpine Research，2018，50（1）：e1447190
[14] LI J X，LI X L，GAO J，et al. Micro-scale fragmentation of the alpine meadow landscape on the Qinghai-Tibet Plateau under external disturbances[J]. Catena，2021，201：105220
[15] SHANG Z H，DONG Q M，SHI J J，et al. Research progress in recent ten years of ecological restoration for ‘black soil land’ degraded grassland on Tibetan Plateau—concurrently discuss of ecological restoration in sangjiangyuan region[J]. Acta Agrestia Sinica，2018，26（1）：1-21 尚占环，董全民，施建军，等. 青藏高原“黑土滩”退化草地及其生态恢复近10年研究进展——兼论三江源生态恢复问题[J]. 草地学报，2018，26（1）：1-21
[16] YAN H Y，ZHANG Y，ZHAO J Z，et al. Effects of plateau pika （Ochotona curzoniae） on plant community biomass in alpine meadow[J]. Acta Theriologica Sinica，2013，33（4）：333-343 严红宇，张毓，赵建中，等. 高原鼠兔对高寒草甸植物群落生物量的影响[J]. 兽类学报，2013，33（4）：333-343
[17] WANG J W，YI J，LIU J L，et al. Diversity of archaea in root soil of desert relict plant Psammophylla[J]. Chinese Journal of Grassland Science，2019，46（1）：117-125 王佳玮，易嘉敏，刘建利，等. 荒漠孑遗植物沙冬青根部土壤古菌多样性[J]. 中国草地学报，2024，46（1）：117-125
[18] WANG Q，WAN J，LI H D，et al. Effects of disturbances by plateau pikas on soil microbial communities in an alpine ecosystem of the southeast Qinghai-Tibetan Plateau， China[J]. European Journal of Soil Biology，2022，113：103442
[19] BATES S T，BERG-LYONS D，CAPORASO J G，et al. Examining the global distribution of dominant archaeal populations in soil[J]. The ISME Journal，2010，5（5）：908-917
[20] CHEN J，GU J D. Faunal burrows alter the diversity， abundance， and structure of AOA，AOB，anammox and n-damo communities in coastal mangrove sediments[J]. Microbial Ecology，2017，74（1）：140-156
[21] PAN H，XIE K X，ZHANG Q C，et al. Archaea and bacteria respectively dominate nitrification in lightly and heavily grazed soil in a grassland system[J]. Biology and Fertility of Soils，2018，54（1）：41-54
[22] HANSEL C M，FENDORF S，JARDINE P M，et al. Changes in bacterial and archaeal community structure and functional diversity along a geochemically variable soil profile[J]. Applied and Environmental Microbiology，2008，74（5）：1620-1633
[23] FLEMING P A，ANDERSON H，PRENDERGAST A S，et al. Is the loss of Australian digging mammals contributing to a deterioration in ecosystem function？[J]. Mammal Review，2014，44（2）：94-108
[24] QIN Y，LIU X Y，HUANG B，et al. Soil microbial communities and the associated effect on soil organic carbon in response to plateau pika bioturbation in alpine grasslands[J]. Global Ecology and Conservation，2023，46：e02561
[25] CLINE L C，ZAK D R，UPCHURCH R A，et al. Soil microbial communities and elk foraging intensity： implications for soil biogeochemical cycling in the sagebrush steppe[J]. Ecology Letters，2017，20（2）：202-211
[26] YANG J W，WANG S J，SU W H，et al. Animal activities of the key herbivore plateau pika （Ochotona curzoniae） on the Qinghai-Tibetan Plateau affect grassland microbial networks and ecosystem functions[J]. Frontiers in Microbiology，2022，13：950811
[27] LENNON E F E，HOULTON B Z. Coupled molecular and isotopic evidence for denitrifier controls over terrestrial nitrogen availability[J]. The ISME Journal，2016，11（3）：727-740
[28] CHE R X，WANG F，WANG W J，et al. Increase in ammonia-oxidizing microbe abundance during degradation of alpine meadows may lead to greater soil nitrogen loss[J]. Biogeochemistry，2017，136（3）：341-352
[29] FIERER N. Embracing the unknown： disentangling the complexities of the soil microbiome[J]. Nature Reviews Microbiology，2017，15（10）：579-590
[30] ZHANG H Y. Effects of plateau zokor-mounds on plant communities and soil nutrients in alpine meadow[D]. Lanzhou：Lanzhou University，2019：22-24 张红艳. 高原鼢鼠鼠丘对高寒草甸植物群落和土壤养分的影响[D]. 兰州：兰州大学，2019：22-24
[31] LI X L，ZHANG Y P，YAN H R，et al. Watershed-level spatial pattern of degraded alpine meadow and its key influencing factors in the Yellow River Source Zone of West China[J]. Ecological Indicators， 2023，146：109865
[32] WANG X，CHEN K L，WANG H S，et al. Characteristics of methane-oxidizing bacteria communities in three types of alpine wetlands in Qinghai Lake[J]. Bulletin of Microbiology，2023，50（10）：4357-4371 王霞，陈克龙，王恒生，等. 青海湖3种类型高寒湿地甲烷氧化菌群落特征[J]. 微生物学通报，2023，50（10）：4357-4371
[33] ZHANG N，CHEN K L，BAO H. Response of methane-oxidizing bacteria community to temperature rise in river source wetland of Qinghai Lake[J]. Ecological Science，2023，42（4）：200-208 章妮，陈克龙，暴涵. 青海湖河源湿地甲烷氧化菌群落对温度升高的响应[J]. 生态科学，2023，42（4）：200-208
[34] ZHANG N，BAO H，ZUO D Z，et al. Community characteristics of methanogens in alpine wetland driven by precipitation changes[J]. Biotechnology Bulletin，2021，37（11）：276-284 章妮，暴涵，左弟召，等. 降水变化驱动下的高寒湿地产甲烷菌群落特征变化[J]. 生物技术通报，2021，37（11）：276-284
[35] ZHANG N，CHEN K L，QI W，et al. The influence of simulated warming on the community characteristics of methanogenic bacteria in Qinghai Lake alpine wetland[J]. Ecological Science，2023，42（4）：163-170 章妮，陈克龙，祁闻，等. 模拟增温对青海湖高寒湿地产甲烷菌群落特征的影响[J]. 生态科学，2023，42（4）：163-170
[36] LAMMEL D R，BARTH G，OVASKAINEN O，et al. Direct and indirect effects of a pH gradient bring insights into the mechanisms driving prokaryotic community structures[J]. Microbiome，2018，6（1）：106
[37] DONG L，LI J J，SUN J，et al. Soil degradation influences soil bacterial and fungal community diversity in overgrazed alpine meadows of the Qinghai-Tibet Plateau[J]. Scientific Reports，2021，11：11538
[38] YU C，PANG X P，WANG Q，et al. Soil nutrient changes induced by the presence and intensity of plateau pika （Ochotona curzoniae） disturbances in the Qinghai-Tibet Plateau，China[J]. Ecological Engineering，2017，106：1-9
[39] TRIPATHI B M，KIM M，KIM Y，et al. Variations in bacterial and archaeal communities along depth profiles of Alaskan soil cores[J]. Scientific Reports，2018，8：504
[40] EBOU A E T，KOUA D K，FOSSOU R K，et al. The influence of bioclimates and soil physicochemical properties on bacterial and archaeal communities from forest ecosystems in Côte d’Ivoire （west Africa）[J]. Forests，2024，15（3）：396
[41] WEI G S，LI M C，SHI W C，et al. Similar drivers but different effects lead to distinct ecological patterns of soil bacterial and archaeal communities[J]. Soil Biology and Biochemistry，2020，144：107759
[42] GAO M H，YU C Y，LIU J Y，et al. High throughput analysis of soil archaeal community diversity in Hulunbuir meadow grassland[J]. Feed Review，2022（5）：8-15 高明华，于春艳，刘金月，等. 呼伦贝尔草甸草原土壤古菌群落多样性高通量分析[J]. 饲料博览，2022（5）：8-15
[43] LI L，CHEN J，HAN X，et al. Grassland Ecosystems of China： A Synthesis and Resume[J]. Typical steppe ecosystem，2020：193-248
[44] ZHONG L，DU R，DING K，et al. Effects of grazing on N₂O production potential and abundance of nitrifying and denitrifying microbial communities in meadow-steppe grassland in northern China[J]. Soil Biology and Biochemistry，2014，69：1-10
[45] ZHOU J Z，XIA B C，TREVES D S，et al. Spatial and resource factors influencing high microbial diversity in soil[J]. Applied and Environmental Microbiology，2002，68（1）：326-334
[46] BLAUD A，PHOENIX G K，OSBORN A M. Variation in bacterial，archaeal and fungal community structure and abundance in High Arctic tundra soil[J]. Polar Biology，2015，38（7）：1009-1024
[47] JIANG Z，XIA W. Utilization of food resources byplateau pikas[J]. Acta Theriologica Sinica，1985（5）：251-262
[48] JIANG Z，XIA W. The niches of yaks， Tibetan sheepand plateau pikas in the alpine meadow ecosystem[J]. Acta Biologica Plateau，1987（6）：115-146
[49] TIAN J Q，CHEN H，DONG X Z，et al. Relationship between archaeal community structure and vegetation type in a Fen on the Qinghai-Tibetan Plateau[J]. Biology and Fertility of Soils，2012，48（3）：349-356
[50] SUN F D，CHEN W Y，LIU L，et al. Effects of plateau pika activities on seasonal plant biomass and soil properties in the alpine meadow ecosystems of the Tibetan Plateau[J]. Grassland Science，2015，61（4）：195-203
[51] LI J，CHEN Y Y，QIAO F Y，et al. Effects of disturbance by plateau pika on the β diversity of an alpine meadow [J]. Chinese Journal of Plant Ecology，2021，45（5）：476-486 李捷，陈莹莹，乔福云，等. 高原鼠兔干扰对高寒草甸β多样性的影响[J]. 植物生态学报，2021，45（5）：476-486
[52] CHU H Y，NEUFELD J D，WALKER V K，et al. The influence of vegetation type on the dominant soil bacteria，Archaea，and fungi in a low Arctic tundra landscape[J]. Soil Science Society of America Journal，2011，75（5）：1756-1765
[53] REN Y，HAN C，YANG H，et al. Study on soil microbial diversity under five urban land scape plants[J]. Soils， 2021，53（4）：746-754 任一，韩畅，杨慧，等. 城市五种景观植物土壤微生物多样性研究[J]. 土壤，2021，53（4）：746-754
[54] YI X M，YI K，FANG K K，et al. Microbial community structures and important associations between soil nutrients and the responses of specific taxa to rice-frog cultivation[J]. Frontiers in Microbiology，2019，10：1752
[55] MUKHTAR H，LIN Y P，LIN C M，et al. Relative abundance of ammonia oxidizing Archaea and bacteria influences soil nitrification responses to temperature[J]. Microorganisms，2019，7（11）：526
[56] TAYLOR A E，ZEGLIN L H，WANZEK T A，et al. Dynamics of ammonia-oxidizing Archaea and bacteria populations and contributions to soil nitrification potentials[J]. The ISME Journal，2012，6（11）：2024-2032
[57] ZHOU Y，JIAO S W，LI N N，et al. Impact of plateau pikas （Ochotona curzoniae） on soil properties and nitrous oxide fluxes on the Qinghai-Tibetan Plateau[J]. PLoS One，2018，13（9）：e0203691
[58] BASHEER S，WANG X Q，FAROOQUE A A，et al. A review of greenhouse gas emissions from agricultural soil[J]. Sustainability，2024，16（11）：4789

Effects of Plateau Pika Disturbance on Soil Archaea Community Characteristics in Alpine Meadow

高原鼠兔干扰对高寒草甸土壤古菌群落特征的影响

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