Effects of Different Land Use Patterns on Soil Fungal Community in Typical Steppe of Southeastern Tibet

doi:10.11733/j.issn.1007-0435.2023.04.007

Acta Agrestia Sinica ›› 2023, Vol. 31 ›› Issue (4): 992-1000.DOI: 10.11733/j.issn.1007-0435.2023.04.007

Effects of Different Land Use Patterns on Soil Fungal Community in Typical Steppe of Southeastern Tibet

WANG Ming-tao¹, ZHAO Yu-hong¹, MIAO Yan-jun¹, MA Su-jie¹, SAI Hen-na¹, XU Ya-mei¹, LEI Bian-xia²

1. Animal Science College, Tibet Agriculture and Animal Husbandry University, Linzhi, Tibet 860000, China;
2. Nyingchi Guangdong Experimental Primary School, Linzhi, Tibet 860000, China

Received:2022-09-22 Revised:2022-12-15 Published:2023-04-28

不同土地利用方式对藏东南典型草原土壤真菌群落的影响

王明涛¹, 赵玉红¹, 苗彦军¹, 马素洁¹, 包赛很那¹, 徐雅梅¹, 雷变霞²

1. 西藏农牧学院动物科学学院, 西藏林芝 860000;
2. 林芝市广东实验小学, 西藏林芝 860000

通讯作者: 赵玉红,E-mail:yuhong19801011@126.com
作者简介:王明涛(1986-),男,汉族,吉林四平人,博士研究生,主要从事高寒生态系统修复及牧草种质资源研究,E-mail:wmt060708@126.com
基金资助:
国家重点研发计划(2021YFD1000304)；国家自然科学基金项目(31860151)；西藏重大科技专项(XZ202101ZD003N)；西藏自治区科技厅重点项目(XZ202001ZY00057G)资助

Abstract

Abstract: Proving the effects of different land use patterns on soil fungal community could provide a theoretical basis for the rational utilization of typical steppe in southeastern Tibet. In this study,the Hordeum vulgare var. Coeleste Linnaeus cropland (HC),Elymus nutans Griseb. artificial grassland (EAG) and enclosure grassland (EG) were selected as the research objects along with the uncultivated natural grassland (NG) as the control,and the changes of fungal community characteristics tested by high-throughput sequencing technology were analyzed. The results showed that compared to the control,the contents of soil organic matter,total nitrogen,nitrate nitrogen and available phosphorus in HC,EAG and EG samples increased significantly,while the Shannon index of fungal community decreased significantly in HC and EAG samples. The order of the number of marker species in different samples of land use was HC(9) >EAG(6) =EG(6) >NG(4). Soil bulk density,pH,organic matter and total nitrogen content were closely related to changes in fungal community diversity. The values of organic matter,total nitrogen,ammonium nitrogen,available potassium and pH were the main factors affecting the fungal composition at phylum level;and at the genus level were the values of soil pH,ammonium nitrogen,soil bulk density,organic matter content and total nitrogen content. Therefore,the long-term use of grassland after reclamation into cropland and artificial grassland is beneficial to soil nutrient content enhancement,and the enclosure technology is suitable for ecological protection and utilization of alpine grassland.

Key words: Reclamation, Enclosure, Physical and chemical properties of soil, High-throughput sequencing, Southeastern Tibet

摘要： 探究不同土地利用方式对土壤真菌群落的影响可为藏东南典型草原合理利用提供理论依据。本试验选取青稞农田(HC)、垂穗披碱草人工草地(EAG)和围封草地(EG)为研究对象，以未开垦天然草地(NG)为对照，采用高通量测序技术分析真菌群落特征变化。结果表明：与对照相比，HC、EAG和EG样地土壤有机质、全氮、硝态氮和速效磷含量显著升高；HC和EAG样地真菌群落香农指数显著降低。各样地差异标志物种数量变化排序为HC(9个)>EAG(6个)=EG(6个)>NG(4个)。土壤容重、pH值、有机质和全氮含量与真菌群落多样性变化密切相关。有机质、全氮、铵态氮、速效钾和pH值是真菌门水平组成的主要影响因子；属水平为土壤pH值、铵态氮、容重、有机质和全氮含量。因此，草原开垦为农田和人工草地后长期利用有利于土壤养分含量提升，围封技术适合高寒草地生态保护与利用。

关键词: 开垦, 围封, 土壤理化性质, 高通量测序, 藏东南

CLC Number:

S154.3

WANG Ming-tao, ZHAO Yu-hong, MIAO Yan-jun, MA Su-jie, SAI Hen-na, XU Ya-mei, LEI Bian-xia. Effects of Different Land Use Patterns on Soil Fungal Community in Typical Steppe of Southeastern Tibet[J]. Acta Agrestia Sinica, 2023, 31(4): 992-1000.

王明涛, 赵玉红, 苗彦军, 马素洁, 包赛很那, 徐雅梅, 雷变霞. 不同土地利用方式对藏东南典型草原土壤真菌群落的影响[J]. 草地学报, 2023, 31(4): 992-1000.

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Effects of Different Land Use Patterns on Soil Fungal Community in Typical Steppe of Southeastern Tibet

不同土地利用方式对藏东南典型草原土壤真菌群落的影响

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