Physiological Responses to Low-Temperature Stress and Cold Resistance Evaluation of 20 Medicago falcata Germplasms

doi:10.11733/j.issn.1007-0435.2026.02.023

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (2): 611-623.DOI: 10.11733/j.issn.1007-0435.2026.02.023

Physiological Responses to Low-Temperature Stress and Cold Resistance Evaluation of 20 Medicago falcata Germplasms

DUAN Wei, MIJIT Munire, YIMIN Jamilaigul, WANG Yu-xiang, LI Qian

1. Key Laboratory of Grassland Resources and Ecology of Western Arid Region Ministry of Education, College of Grassland Science Xinjiang Agricultural University, Urumqi, Xinjiang 830052, China

Received:2025-04-10 Revised:2025-05-21 Published:2026-01-22

20份黄花苜蓿种质低温胁迫生理响应及抗寒性评价

段伟, 穆妮热·米吉提, 加米丽古丽·伊明, 王玉祥, 李倩

1. 西部干旱荒漠区草地资源与生态教育部重点实验室, 新疆农业大学草业学院, 新疆乌鲁木齐 830052

通讯作者: 李倩，E-mail:540794898@qq.com
作者简介:段伟（2001-），男，汉族，湖南衡阳人，硕士研究生，主要从事草种质资源育种研究，E-mail:1929674782@qq.com；
基金资助:
新疆维吾尔自治区重点研发项目（2023B02031）；自治区人才发展基金“天池英才”引进计划项目；作物学博士后流动站资助

Abstract

Abstract: To screen cold-tolerant Medicago falcata germplasms and to clarify their physiological response mechanisms to low-temperature stress， this study systematically evaluated the physiological and chlorophyll fluorescence characteristics of 20 accessions under gradient low-temperature stresses （25℃， 10℃， 5℃， 0℃）. By measuring seven key indicators， it was found that the contents of malondialdehyde （MDA）， soluble sugar （SS）， and proline （Pro）， as well as the level of initial fluorescence （F_o）， exhibited upward trends with decreasing temperature， all significantly increasing at 0℃ （P<0.05）； while the relative chlorophyll content （Soil and plant analyzer development， SPAD）， maximum fluorescence （F_m）， and maximum photochemical efficiency of PSⅡ （Maximal photochemical efficiency of photosystem II， F_v/F_m） showed downward trends. A comprehensive evaluation using the membership function method combined with the standard deviation coefficient weighting method identified M₄， M₁， and M₁₀ as accessions with stronger cold tolerance. Stepwise regression analysis further established a cold tolerance prediction model （R²=0.932）， confirming F_v/F_m and SS content as critical evaluation indices. These results provide excellent germplasm resources and a theoretical basis for cold-tolerance breeding of alfalfa， and it is recommended that future research integrate omics technologies to further elucidate the underlying molecular regulatory networks.

Key words: Medicago falcata, Low-temperature stress, Physiological response, Chlorophyll fluorescence, Cold resistance evaluation

摘要： 为筛选耐寒型黄花苜蓿（Medicago falcata）材料并阐明其低温生理响应机制，本研究系统评价了20份种质在梯度低温胁迫（25℃，10℃，5℃，0℃）下的生理及叶绿素荧光特性。通过测定7项关键指标发现：随温度降低，丙二醛（Malondialdehyde，MDA）、可溶性糖（Soluble sugar，SS）、脯氨酸（Proline，Pro）含量及初始荧光（Initial fluorescence，F_o）呈上升趋势，在0℃均显著增加（P<0.05）；而叶绿素相对含量（Soil and plant analyzer development，SPAD）、最大荧光（Maximum fluorescence，F_m）和PSⅡ最大光化学效率（Maximal photochemical efficiency of photosystem II，F_v/F_m）呈下降趋势。基于隶属函数法结合标准差系数赋权法的综合评价表明，M₄，M₁，M₁₀等种质表现出较强耐寒性。逐步回归分析进一步建立了耐寒性预测模型（R2=0.932），确定F_v/F_m和SS含量为关键评价指标。研究结果为苜蓿抗寒育种提供了优异种质和理论依据，建议后续结合组学等技术深入解析其分子调控网络。

关键词: 黄花苜蓿, 低温胁迫, 生理响应, 叶绿素荧光, 耐寒性评价

DUAN Wei, MIJIT Munire, YIMIN Jamilaigul, WANG Yu-xiang, LI Qian. Physiological Responses to Low-Temperature Stress and Cold Resistance Evaluation of 20 Medicago falcata Germplasms[J]. Acta Agrestia Sinica, 2026, 34(2): 611-623.

段伟, 穆妮热·米吉提, 加米丽古丽·伊明, 王玉祥, 李倩. 20份黄花苜蓿种质低温胁迫生理响应及抗寒性评价[J]. 草地学报, 2026, 34(2): 611-623.

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Physiological Responses to Low-Temperature Stress and Cold Resistance Evaluation of 20 Medicago falcata Germplasms

20份黄花苜蓿种质低温胁迫生理响应及抗寒性评价

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