Effects of Brassinolide on Growth and Physiological Characteristics of Alfalfa under Low Temperature Stress

doi:10.11733/j.issn.1007-0435.2026.04.030

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (4): 1492-1501.DOI: 10.11733/j.issn.1007-0435.2026.04.030

Effects of Brassinolide on Growth and Physiological Characteristics of Alfalfa under Low Temperature Stress

MA Xiang-xiang, NAN Li-li, YANG Li-li, WANG Li-qun, TIAN Ge, GUO Jia-yu

College of Pratacultural Science, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Ministry of Education/Key Laboratory of Forage Gerplasm Innovation and New Variety Breeding of Ministry of Agriculture and Rural Affairs, Lanzhou, Gansu Province 730070, China

Received:2025-06-16 Revised:2025-08-04 Published:2026-04-15

油菜素内酯对低温胁迫下紫花苜蓿生长及生理特性的影响

马香香, 南丽丽, 杨丽丽, 王利群, 田戈, 郭佳雨

甘肃农业大学草业学院/草业生态系统教育部重点实验室/农业农村部饲草种质创新与新品种选育重点实验室, 甘肃兰州 730070

通讯作者: 南丽丽，E-mail：nanll@gsau.edu.cn
作者简介:马香香（1998-），女，汉族，安徽滁州人，硕士研究生，主要从事牧草栽培与育种研究，E-mail：1963962817@qq.com；
基金资助:
油菜秸秆与紫花苜蓿混贮效果研究及示范推广（ZC-STK-2024A-013）；国家现代农业产业技术体系（CARS-34）共同资助

Abstract

Abstract: In order to explore the effects of spraying brassinosteroid （BR） on the growth and physiological characteristics of alfalfa （Medicago sativa） seedlings under low temperature stress， the new cold-resistant alfalfa line （P4） and Longdong alfalfa （Medicago sativa ‘Longdong’） were used as the research objects in this study. After 13 days of low temperature stress （4℃）， different concentrations of exogenous BR （0.00， 0.25， 0.50， 0.75， and 1.00 mg·L^-1， representing T₀， T₁， T₂， T₃， T₄ treatment， respectively） were sprayed on the leaves. After 5 days， the morphological and physiological indexes of the seedlings were measured. The results showed that low temperature stress significantly inhibited the growth of alfalfa seedlings. However， after applying different concentrations of BR， the plant height， leaf length， leaf width， leaf area， aboveground and underground biomass， root shoot ratio， total root length， total root surface area， root diameter， root volume， root tip number， root activity， phenylalanine ammonia lyase， cinnamic acid-4-hydroxylase， 4-coumaric acid coenzyme A ligase and total flavonoid content of alfalfa seedlings were significantly higher than those of T₀ （P<0.05）. The content of malondialdehyde decreased significantly （P<0.05）. The comprehensive evaluation of membership function showed that different concentrations of BR treatments were in the order of T₂>T₁>T₃>T₄>T₀， indicating that spraying 0.25-1.00 mg·L^-1 BR could alleviate the effects of low temperature on the growth of alfalfa seedlings， while the 0.5 mg·L^-1 （T₂） BR treatment was the best. The results provided a theoretical basis for the rational use of BR to solve the problem of chilling injury in alfalfa cultivation.

Key words: Alfalfa, Low temperature stress, Brassinosteroid, Physiological Characteristics, Membership Function

摘要： 为探明低温胁迫下喷施油菜素内酯（Brassinosteroid，BR）对紫花苜蓿（Medicago sativa）幼苗生长和生理特性的影响，本研究以抗寒苜蓿新品系（P4）和‘陇东苜蓿’（Medicago sativa ‘Longdong’）为研究对象，低温胁迫（4℃）13 d后，对叶面喷施不同浓度的外源BR（0.00，0.25，0.50，0.75，1.00 mg·L^-1，分别代表T₀，T₁，T₂，T₃，T₄处理），5天后测定其幼苗形态和生理指标。结果表明：低温胁迫显著抑制了苜蓿幼苗的生长；然而，施加不同浓度BR后，供试苜蓿幼苗株高、叶长、叶宽、叶面积、地上与地下生物量、根冠比、根系总长度、根总表面积、根直径、根体积、根尖数、根系活力、苯丙氨酸解氨酶、肉桂酸-4-羟基化酶、4-香豆酸辅酶Ａ连接酶和总黄酮含量指标均显著高于T₀（P<0.05）；丙二醛含量显著降低（P<0.05）。隶属函数综合评价显示不同浓度BR处理依次表现为：T₂>T₁>T₃>T₄>T₀，表明喷施0.25~1.00 mg·L^-1的BR均能缓解低温对苜蓿幼苗生长的影响，但以0.5 mg·L^-1（T₂）的BR处理效果最优，其研究结果为合理利用BR解决苜蓿栽培中的冷害问题提供了理论依据。

关键词: 紫花苜蓿, 低温胁迫, 油菜素内酯, 生理特性, 隶属函数

CLC Number:

S541.9

MA Xiang-xiang, NAN Li-li, YANG Li-li, WANG Li-qun, TIAN Ge, GUO Jia-yu. Effects of Brassinolide on Growth and Physiological Characteristics of Alfalfa under Low Temperature Stress[J]. Acta Agrestia Sinica, 2026, 34(4): 1492-1501.

马香香, 南丽丽, 杨丽丽, 王利群, 田戈, 郭佳雨. 油菜素内酯对低温胁迫下紫花苜蓿生长及生理特性的影响[J]. 草地学报, 2026, 34(4): 1492-1501.

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Effects of Brassinolide on Growth and Physiological Characteristics of Alfalfa under Low Temperature Stress

油菜素内酯对低温胁迫下紫花苜蓿生长及生理特性的影响

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