Identification and Stress-Induced Expression Profiling of Stay-Green Genes CiSGR and CiSGRL in Chicory

doi:10.11733/j.issn.1007-0435.2026.06.008

Acta Agrestia Sinica ›› 2026, Vol. 34 ›› Issue (6): 2043-2054.DOI: 10.11733/j.issn.1007-0435.2026.06.008

Identification and Stress-Induced Expression Profiling of Stay-Green Genes CiSGR and CiSGRL in Chicory

XIE Ming-yu¹, LIU Xue^2,3, WANG Chen-chen⁴, FENG Qing-xiang⁴, LI Da-yong^2,3, ZHANG De-chun¹, ZHANG Bin^2,3

1. College of Biology and Pharmaceutical, China Three Gorges University, Yichang, Hubei Province 443000, China;
2. National Engineering Research Center for Vegetables, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China;
3. Beijing Key Laboratory of Crop Molecular Design and Intelligent Breeding, Beijing 100097, China;
4. College of Life Sciences, Shandong Normal University, Jinan, Shandong Province 250014, China

Received:2025-06-16 Revised:2025-10-28 Published:2026-06-02

菊苣持绿基因CiSGR和CiSGRL的鉴定及胁迫条件下的表达分析

谢明宇¹, 刘雪^2,3, 王晨晨⁴, 冯清香⁴, 李大勇^2,3, 张德春¹, 张彬^2,3

1. 三峡大学生物与制药学院, 湖北宜昌 443000;
2. 北京市农林科学院蔬菜研究所, 国家蔬菜工程技术研究中心, 北京 100097;
3. 北京市农林科学院蔬菜研究所, 作物分子设计与智慧育种北京市重点实验室, 北京 100097;
4. 山东师范大学生命科学学院, 山东济南 250014

通讯作者: 张彬,E-mail:zhangbin@nercv.org;张德春,E-mail:zhangdechun@ctgu.edu.cn
作者简介:谢明宇(2001-),男,满族,河北承德人,硕士研究生,主要从事植物基因组学研究,E-mail:921947629@qq.com
基金资助:
北京市农林科学院创新能力建设专项科技攻关项目（KJCX20251008）；北京市农林科学院蔬菜研究所改革与发展项目（KYCX202604）资助

Abstract

Abstract: Chicory （Cichorium intybus L.） is a perennial forage extensively utilized in livestock production. The magnesium-dechelatase involved in chlorophyll degradation plays a pivotal rolein regulating chlorophyll breakdown and is a key factor in the control of stay-green traits of plants. Enhancing the stay-green phenotype is critical for improving chicory’s resilience to environmental stresses. In this study， two putative SGR （Stay-Green） genes were preliminarily identified in the chicory genome， and designated as CiSGR and CiSGRL based on their respective phylogenetic affiliations to the SGR and SGRL subfamilies. The proteins encoded by these genes were composed of 274 and 232 amino acids， respectively. Structural domain analysis revealed that both CiSGR and CiSGRL possessed chloroplast transit peptide domains， highly conserved SGR domains， and variable regions at the C-terminus. Cis-regulatory element analysis indicated that the promoters of CiSGR and CiSGRL contained light-responsive， hormone-related， and stress-inducible transcriptional elements. Subcellular localization assays demonstrated that CiSGR predominantly localized to chloroplasts and nuclei， whereas CiSGRL primarily resided in nuclei and cytoplasm. Tissue-specific expression profiling showed the highest transcript levels of both genes in stems and significant induction under six different abiotic stress conditions. This systematic identification and analysis of chicory SGR and SGRL genes provides a theoretical foundation for strategies aimed at improving chicory forage yield and quality.

Key words: Cichorium intybus, SGR gene family, Forage grass, Stress response

摘要： 菊苣（Cichorium intybus L.）是一种被广泛用于畜牧业中的多年生牧草。持绿蛋白脱镁螯合酶参与调控叶绿素的分解，是植物调控持绿性状的关键因子，对提高菊苣的抗逆性至关重要。本研究在菊苣基因组中初步筛选到了2个菊苣SGR基因，这两个基因编码的蛋白质分别属于SGR亚家族和SGRL亚家族，分别将其命名为CiSGR和CiSGRL。二者编码的蛋白质分别由274和232个氨基酸组成。蛋白结构域分析表明，CiSGR和CiSGRL具有叶绿体转运肽结构域、高度保守的SGR结构域和C端可变区。顺式元件分析表明，CiSGR和CiSGRL的启动子含有光、激素和胁迫相关转录元件。亚细胞定位表明CiSGR主要定位于叶绿体和细胞核，CiSGRL主要定位于细胞核和细胞质。组织表达模式分析发现，CiSGR和CiSGRL均在茎中表达量最高，并受到6种不同胁迫信号的显著诱导。本研究对菊苣SGR和SGRL基因进行了系统鉴定和分析，为提升菊苣牧草的产量与品质提供理论依据。

关键词: 菊苣, SGR基因家族, 饲草, 胁迫反应

CLC Number:

Q943.2

XIE Ming-yu, LIU Xue, WANG Chen-chen, FENG Qing-xiang, LI Da-yong, ZHANG De-chun, ZHANG Bin. Identification and Stress-Induced Expression Profiling of Stay-Green Genes CiSGR and CiSGRL in Chicory[J]. Acta Agrestia Sinica, 2026, 34(6): 2043-2054.

谢明宇, 刘雪, 王晨晨, 冯清香, 李大勇, 张德春, 张彬. 菊苣持绿基因CiSGR和CiSGRL的鉴定及胁迫条件下的表达分析[J]. 草地学报, 2026, 34(6): 2043-2054.

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Identification and Stress-Induced Expression Profiling of Stay-Green Genes CiSGR and CiSGRL in Chicory

菊苣持绿基因CiSGR和CiSGRL的鉴定及胁迫条件下的表达分析

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