Identification of the Genetic Diversity and Relationship of Medicago Germplasms by Two Kinds of SSR Markers

doi:10.11733/j.issn.1007-0435.2008.06.004

›› 2008, Vol. 16 ›› Issue (6): 559-565.DOI: 10.11733/j.issn.1007-0435.2008.06.004

Previous Articles Next Articles

Identification of the Genetic Diversity and Relationship of Medicago Germplasms by Two Kinds of SSR Markers

YANG Zhan-hua¹, WEI Zhen-wu², LEI Yan-fang¹, ZHANG Li-fang¹

1. Pratacultural College, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China;
2. College of Animal Science & Technique, Yangzhou University, Yangzhou, Jiangsu Province 225009, China

Received:2008-12-27 Revised:2008-04-15 Online:2008-12-15 Published:2008-12-15

两类SSR对苜蓿属种质遗传多样性和亲缘关系的比较研究

杨占花¹, 魏臻武², 雷艳芳¹, 张丽芳¹

1. 甘肃农业大学草业学院, 兰州, 730070;
2. 扬州大学动物科学与技术学院, 扬州, 225009

通讯作者: 魏臻武,E-mail:zhen-wu_wei@yahoo.com.cn
作者简介:杨占花(1983- ),女,青海乐都人,硕士研究生,研究方向为牧草遗传育种.
基金资助:
国家自然科学基金(30671486);甘肃省教育厅科学技术研究项目;甘肃农业大学草业生态系统教育部省部共建重点实验室基金

Abstract

Abstract: The genetic diversity and kinship of 38 species of Medicago L.were analyzed using 33 nuSSR markers and 28 chloroplast microsatellites(cpSSR).In 38 species of Medicago L.,the average alleles of 28 chloroplast SSR markers was higher than 33 nucler SSR markers,while the genetic diversity index was opposite.The result of three clustering for 38 species of medicago L.using 33 nucleus SSR,28 chloroplast SSR,and 61 locus was as follows: three clustering separated all materials into two types,i.e.annual Medicago species and perennial Medicago species.Accessions of annual Medicago species were the more genetically diverse and contained larger number of regional special alleles,especially M.truncatula Gaertn.,than perennial Medicago species.

Key words: Perennial Medicago species, Annual Medicago species, nuSSR, cpSSR, Genetic diversity

摘要： 利用33对细胞核SSR标记和28对叶绿体SSR标记对38份苜蓿属种质进行了遗传多样性和亲缘关系分析。在38份苜蓿属种质中,叶绿体SSR标记的平均等位变异数高于细胞核SSR标记,而遗传多样性指数则相反;分别用细胞核SSR、叶绿体SSR和61对SSR标记对38份苜蓿属种质进行聚类,结果显示:3次聚类都把38份苜蓿属种质划分为一年生和多年生苜蓿两个大类群;以相似系数0.8为准,3次聚类结果的差异主要显示在一年生苜蓿内部尤其是蒺藜苜蓿种质内的品种(系)划分上。

关键词: 多年生苜蓿, 一年生苜蓿, nuSSR, cpSSR, 遗传多样性

CLC Number:

S812
S326

YANG Zhan-hua, WEI Zhen-wu, LEI Yan-fang, ZHANG Li-fang. Identification of the Genetic Diversity and Relationship of Medicago Germplasms by Two Kinds of SSR Markers[J]. , 2008, 16(6): 559-565.

杨占花, 魏臻武, 雷艳芳, 张丽芳. 两类SSR对苜蓿属种质遗传多样性和亲缘关系的比较研究[J]. , 2008, 16(6): 559-565.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2008.06.004

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2008/V16/I6/559

References

[1] 毕玉芬.论苜蓿属植物遗传资源多样性及其保护问题[J].国外畜牧学-草原与草坪,1997,(4):1-5
[2] 卢欣石,何琪.中国苜蓿品种资源遗传多样性研究[J].中国草地,1997,(6):1-6
[3] 于林清,王照兰,萨仁,等.黄花苜蓿野生种群遗传多样性的初步研究[J].中国草地,2001,23(1):23-25
[4] 毕玉芬.北疆苜蓿属植物遗传多样性分析[J].草业科学,1998,15(3):13-18
[5] 魏臻武,曹致中.苜蓿品种特征特性及其遗传分子标记[A].见:中国草学会牧草遗传资源专业委员会编.新世纪牧草遗传资源研究[C].呼和浩特:内蒙古人民出版社,2003,157-167
[6] 魏臻武,盖钧镒.豆科模式植物蒺藜苜蓿基因组研究进展[J].中国草地学报,2006,(6):83-90
[7] Brummer E C,Bouton J H,Kochert G.Development of an RFLP map in diploid alfalfa[J].Theor.Appl.,1993,86:329-332
[8] Cregan P B,Bhagwat A A,Akkaya M S,et al.Microsatellite fingerprinting and mapping of soybean[J].Method.Mol.Cell.Biol.,1994,5:49-61
[9] Joshi S P,Gupta V S,Aggarwal R K,et al.Genetic diversity and phylogenetic relationship as revealed by inter-simple sequence repeat (ISSR) polymorphism in the genus Oryza[J].Theor.Appl.Genet.,2000,100:1311-1320
[10] 李拥军.苜蓿地方品种遗传多样性的研究-RAPD标记[J].草地学报,1998,(2):105-114
[11] 李拥军,苏加楷.中国苜蓿地方品种亲缘关系的研究-RAPD标记[J].草业学报,1999,(3):46-53
[12] 魏臻武.利用SSR、ISSR和RAPD技术构建苜蓿基因组DNA指纹图谱[J].草业学报,2004,3:62-67
[13] Diwan N,Bhagwat A,Bauchan G,et al.Simple sequence repeat DNA markers in alfalfa and perennial and annual Medicago species[J].Genome,1997,40:887-895
[14] Ellwood S R,Souza N K,Kamphuis L G,et al.SSR analysis of the Medicago truncatula SARDI core collection reveals substantial diversity and unusual genotype dispersal throughout the Mediterranean basin[J].Theor.Appl.Genet.,2006,112:977-983
[15] 魏臻武,符昕,耿小丽,等.苜蓿遗传多样性和亲缘关系的SSR和ISSR分析[J].草地学报,2007,15(2):118-123
[16] 毕玉芬,车伟光,李季蓉.利用RAPD 技术研究弱秋眠性紫花苜蓿遗传多样性[J].作物学报,2005,32(5):647-652
[17] 蒿若超,张月学,唐凤兰.利用RAPD标记研究了53份苜蓿种质资源遗传多样性[J].草业科学,2007,24(8):69-73
[18] 刘志鹏,杨青川,呼天明,等.用SSR标记研究不同耐盐特性四倍体紫花苜蓿的遗传多样性[J].作物学报,2006,32(4):630-632
[19] Choi H K,Kim D,Uhm T,et al.A sequence-based genetic map of Medicago truncatula and comparison of marker colinearity with M.sativa[J].Genetics,2004,166(3):1463-1502
[20] Powell W,Morgante M,Doyle J J,et al.Genepool variation in genus Glycine subgenus Soja revealed by polymorphic nuclear and chloroplast microsatellites[J].Genetics,1996,144:793-803
[21] Mengoni A,Ruggini C,Vendramin G G,et al.Chloroplast microsatellite variations in tetraploid alfalfa[J].Plant Breed,2000,119(6):509-512
[22] Mengoni A,Gori A,Bazzicalupo M.Use of RAPD and microsatellite (SSR) variation to assess genetic relationships among populations of tetraploid alfalfa (Medicago sativa)[J].Plant Breed,2001,119:311-317
[23] 张丽芳,魏臻武,杨占花.蒺藜苜蓿SSR反应体系优化及在一年生苜蓿种质鉴定中的应用[J].草地学报,2007,15(5):429-436
[24] 海林,王克晶.半野生大豆种质资源SSR位点遗传多样性分析[J].西北植物学报,2002,22(4):751-757

Identification of the Genetic Diversity and Relationship of Medicago Germplasms by Two Kinds of SSR Markers

两类SSR对苜蓿属种质遗传多样性和亲缘关系的比较研究

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	MA Cong-yu, HAN Chong-yang, MA Sai-nan, LI Xu-xu, CAI Jia-bang, WANG Yang, ZHANG Xin-quan, NIE Gang. The Fingerprints of 13 White Clover (Trifolium repens L.) Cultivars Based on SSR Markers [J]. Acta Agrestia Sinica, 2021, 29(9): 1892-1899.
[2]	LUO Ming-xin, LIU Feng-min, CHEN Ji-yan, CUI Jun-tao, ZHANG Wei-li. Agronomic Traits and Genetic Diversity Analysis of M₃ Generation of Stylosanthes spp. Irradiated by ⁶⁰Coγ [J]. Acta Agrestia Sinica, 2021, 29(9): 1992-2000.
[3]	LIANG Xiao-yu, JI Yang, HU Yuan-bin, YI Jun, BAI Shi-qie, ZHANG Jing, ZHANG Xin-quan. Exploitation and Universal Analysis of EST-SSR Molecular Markers in Cichorium intybus [J]. Acta Agrestia Sinica, 2021, 29(9): 2081-2090.
[4]	LIU Ling-yun, FAN Xi-feng, TENG Ke, YUE Yue-sen, CHANG Zhi-hui, WU Ju-ying. Advances in Development of Genetic Diversity of Carex L. [J]. Acta Agrestia Sinica, 2021, 29(6): 1148-1157.
[5]	CHEN Zhi-xiang, LUO Xiao-yan, LI Shuan-lin, WU Ru-yue, WANG Wen-qiang, DING Xi-peng. Genetic Diversity and Population Structure Analysis of the Pigeonpea (Cajanus cajanL.) Germplasm Resources Based on SSR Markers [J]. Acta Agrestia Sinica, 2021, 29(5): 904-911.
[6]	LIANG Guo-ling, LIU Wen-hui, MA Xiang. Phenotypic Diversity of the Panicle Among 590 Covered Oats Germplasm Resources in Apline of Qinghai-Tibet Plateau [J]. Acta Agrestia Sinica, 2021, 29(3): 495-503.
[7]	ZHANG Qi, WEI Zhen-wu, YAN Tian-fang, GENG Xiao-li. Identification and Evaluation of Genetic Diversity of Agronomic Traits in Oat Germplasm Resources [J]. Acta Agrestia Sinica, 2021, 29(2): 309-316.
[8]	AN Ming-zhu, HAN Bo, JIANG Hua, DUAN Xin-hui, WEN Yi-fu, ZHOU Kai. Research Progress of Dactylis Species Origin and Phylogeny Analysis [J]. Acta Agrestia Sinica, 2021, 29(12): 2637-2644.
[9]	WANG Jian-li, LIU Jie-lin, ZHU Rui-fen, ZHONG Peng, YOU Jia, DI Gui-li, HAN Wei-bo, SHEN Zhong-bao. Genetic Diversity Analysis of Major Agronomic Traits in 28 Amaranthus hypochondriacus Germplasm Resources [J]. Acta Agrestia Sinica, 2020, 28(4): 1050-1059.
[10]	YANG Yan-ting, SHI Feng-ling, XU Bo, ZHANG Yu-tong, YAN Wei. Identification and Genetic Diversity of Alfalfa Varieties [J]. Acta Agrestia Sinica, 2020, 28(4): 1060-1067.
[11]	MA Dong-mei, ZHANG Xi-bin, HUANG Ting, WANG Weng-jing, ZHAO Li-juan, MA Qiao-li. Genetic Diversity Analysis of 10 Salt Tolerant Alfalfa [J]. Acta Agrestia Sinica, 2019, 27(6): 1477-1485.
[12]	LIU Ya-ling, GENG Ya-ping, XIE Xiao-dong, WANG Fang, ZHANG Peng-fei. Genetic Diversity and Genetic Structure Analysis of Astragalus Based on SSR Molecular Marker [J]. Acta Agrestia Sinica, 2019, 27(5): 1154-1162.
[13]	DENG Zhen-Shan, LI Mai-ping, HAO Lei, CHEN Kai-kai, LI Jing, LIU Yu-zhen, ZHANG Bao-bao, QI Xiang-ying. Diversity and Plant Growth Promoting Activities of the Culturable Endophytic Bacteria Associated with Pennisetum sp. [J]. Acta Agrestia Sinica, 2019, 27(5): 1213-1221.
[14]	XU Zhen-peng, WAN Tao, CAI Ping, ZHANG Xiao-ming, YI Wei-dong, WAN Yi-chao. Study on the Relevance between Genetic Diversity of Gymnocarpos przewalskii Populations and Geographic Climatic Factors [J]. Acta Agrestia Sinica, 2018, 26(1): 70-76.
[15]	NAN Ming, ZHAO Gui-Qin, CHAI Ji-Kuan. Phenotypic Diversity and Comprehensive Evaluations of Avena sativa L. Germplasm in Semi-arid Area of the Loess Plateau [J]. Acta Agrestia Sinica, 2017, 25(6): 1197-1205.