Mycosystema

Figure/Table detail

Difference of nutritional components and metabolites in basidiomata of Auricularia cornea cultivated with three different substrates

LUO Yanglan, CHEN Lixin, WANG Canqin, WEI Jiaojun, LAN Taojü, WU Shengjin, WEI Shiyan

Mycosystema, 2024, 43(5): 230222. DOI: 10.13346/j.mycosystema.230222

试验组 Group	序号 Serial number	代谢通路 Pathway	代谢物数目 Number of metabolites	P值 P-value	参与差异代谢物 Metabolite
B&A	1	氨基糖和核苷酸糖代谢 Amino sugar and nucleotide sugar metabolism	5	<0.001	甘露糖-6-磷酸Mannose 6-phosphate；尿苷二磷酸-N-乙酰氨基葡萄糖 Uridine diphosphate-N-acetylglucosamine；尿苷二磷酸葡糖醛酸 Uridine diphosphate glucuronic acid；尿苷二磷酸木糖UDP-xylose；l-葡萄糖 l-glucose
	2	淀粉和蔗糖代谢 Starch and sucrose metabolism	3	<0.001	蔗糖Sucrose；海藻糖-6-磷酸Trehalose-6-phosphate； l-葡萄糖 l-glucose
	3	丙氨酸、天冬氨酸和谷氨酸代谢 Alanine, aspartate and glutamate metabolism	2	<0.001	γ氨基丁酸Gamma-aminobutyric acid； d-天冬氨酸 d-aspartic acid
	4	ABC转运蛋白 ABC transporters	3	0.012	甜菜碱Betaine；蔗糖Sucrose； l-葡萄糖 l-glucose
	5	半乳糖代谢 Galactose metabolism	2	0.013	蔗糖Sucrose；l-葡萄糖 l-glucose
C&A	1	丙氨酸、天冬氨酸和谷氨酸代谢 Alanine, aspartate and glutamate metabolism	5	<0.001	l-谷氨酸l-glutamic acid；l-谷氨酰胺l-glutamine； d-天冬氨酸d-aspartic acid；柠檬酸Citric acid； γ氨基丁酸Gamma-aminobutyric acid
	2	玉米素生物合成 Zeatin biosynthesis	3	0.001	尿苷-5ʹ-二磷酸钠盐Uridine 5ʹ-diphosphate；尿苷二磷酸木糖UDP-xylose；单磷酸腺苷Adenosine monophosphate
	3	氨基糖和核苷酸糖代谢 Amino sugar and nucleotide sugar metabolism	4	0.003	甘露糖-6-磷酸Mannose 6-phosphate；尿苷二磷酸葡糖醛酸 Uridine diphosphate glucuronic acid；尿苷二磷酸木糖UDP-xylose；尿苷二磷酸-N-乙酰氨基葡萄糖 Uridine diphosphate-N-acetylglucosamine
	4	ABC转运蛋白 ABC transporters	4	0.005	蔗糖Sucrose；甜菜碱Betaine； l-谷氨酸l-glutamic acid；l-谷氨酰胺l-glutamine
	5	乙醛酸和二羧酸代谢 Glyoxylate and dicarboxylate metabolism	3	0.005	柠檬酸Citric acid； l-谷氨酸l-glutamic acid； l-谷氨酰胺l-glutamine
	6	嘧啶代谢 Pyrimidine metabolism	3	0.005	5ʹ-单磷酸尿苷Uridine 5ʹ-monophosphate；尿苷-5′-二磷酸钠盐Uridine 5ʹ-diphosphate； l-谷氨酰胺l-glutamine
	7	氮代谢 Nitrogen metabolism	2	0.006	l-谷氨酸l-glutamic acid； l-谷氨酰胺l-glutamine
	8	精氨酸生物合成 Arginine biosynthesis	2	0.008	l-谷氨酸l-glutamic acid； l-谷氨酰胺l-glutamine
	9	嘌呤代谢 Purine metabolism	3	0.014	腺苷Adenosine；l-谷氨酰胺l-glutamine；单磷酸腺苷Adenosine monophosphate
	10	C5-支链二元酸代谢 C5-Branched dibasic acid metabolism	2	0.016	l-谷氨酸l-glutamic acid；衣康酸Itaconic acid
	11	谷胱甘肽代谢 Glutathione metabolism	2	0.019	l-谷氨酸l-glutamic acid；焦谷氨酸Pyroglutamic acid
	12	淀粉和蔗糖代谢 Starch and sucrose metabolism	2	0.019	蔗糖Sucrose；海藻糖-6-磷酸Trehalose-6-phosphate
	13	丁酸代谢 Butanoate metabolism	2	0.023	γ氨基丁酸gamma-Aminobutyric acid； l-谷氨酸l-glutamic acid
	14	氨基酸生物合成 Biosynthesis of amino acids	3	0.030	柠檬酸Citric acid； l-谷氨酸l-glutamic acid； l-谷氨酰胺l-glutamine
	15	氨酰基-tRNA生物合成 Aminoacyl-tRNA biosynthesis	2	0.032	l-谷氨酸l-glutamic acid； l-谷氨酰胺l-glutamine
B&C	1	FoxO信号通路 FoxO signaling pathway	2	<0.001	l-葡萄糖 l-glucose；单磷酸腺苷Adenosine monophosphate
	2	环腺苷酸信号通路 cAMP signaling pathway	3	<0.001	γ氨基丁酸Gamma-aminobutyric acid；腺苷Adenosine；单磷酸腺苷Adenosine monophosphate
	3	丙氨酸、天冬氨酸和谷氨酸代谢 Alanine, aspartate and glutamate metabolism	3	<0.001	柠檬酸Citric acid； l-谷氨酰胺l-glutamine； γ氨基丁酸Gamma-aminobutyric acid
	4	GABA能神经突触 GABA ergic synapse	2	0.001	γ氨基丁酸Gamma-aminobutyric acid； l-谷氨酰胺l-glutamine
	5	淀粉和蔗糖代谢 Starch and sucrose metabolism	3	0.001	海藻糖-6-磷酸Trehalose-6-phosphate；蔗糖Sucrose；l-葡萄糖 l-glucose
	6	cGMP-PKG信号通路 cGMP-PKG signaling pathway	2	0.003	腺苷Adenosine；单磷酸腺苷Adenosine monophosphate
	7	脂肪细胞脂解的调控 Regulation of lipolysis in adipocytes	2	0.003	腺苷Adenosine；单磷酸腺苷Adenosine monophosphate
	8	鞘脂信号通路 Sphingolipid signaling pathway	2	0.003	腺苷Adenosine；鞘氨醇Sphinganine
	9	腺苷酸激活蛋白激酶信号通路 AMPK signaling pathway	2	0.006	l-葡萄糖 l-glucose；单磷酸腺苷Adenosine monophosphate
	10	胰高血糖素信号通路 Glucagon signaling pathway	2	0.008	l-葡萄糖 l-glucose；柠檬酸Citric acid
	11	嘌呤代谢 Purine metabolism	3	0.014	腺苷Adenosine；单磷酸腺苷Adenosine monophosphate； l-谷氨酰胺l-glutamine
	12	ABC转运蛋白 ABC transporters	3	0.020	l-谷氨酰胺l-glutamine； l-葡萄糖 l-glucose；蔗糖Sucrose
	13	神经活性配体-受体相互作用 Neuroactive ligand-receptor interaction	3	0.022	γ氨基丁酸Gamma-aminobutyric acid；尿苷-5′-二磷酸钠盐Uridine 5ʹ-diphosphate；腺苷Adenosine
	14	mTOR信号通路 mTOR signaling pathway	1	0.024	单磷酸腺苷Adenosine monophosphate
	15	磷脂酰肌醇3-激酶-蛋白激酶通路 PI3K-Akt signaling pathway	1	0.024	单磷酸腺苷Adenosine monophosphate
	16	胰岛素信号通路 Insulin signaling pathway	1	0.024	l-葡萄糖 l-glucose
	17	氨基糖和核苷酸糖代谢 Amino sugar and nucleotide sugar metabolism	3	0.028	尿苷二磷酸-N-乙酰氨基葡萄糖 Uridine diphosphate-N-acetylglucosamine； l-葡萄糖 l-glucose；甘露糖-6-磷酸Mannose 6-phosphate
	18	PPAR信号通路 PPAR signaling pathway	1	0.029	9-羟基-10,12-十八碳二烯酸 9-Hydroxy-10,12-octadecadienoic acid
	19	谷氨酸能突触 Glutamatergic synapse	1	0.042	l-谷氨酰胺l-glutamine
	20	嗅觉传导通路 Olfactory transduction	1	0.042	单磷酸腺苷Adenosine monophosphate
	21	雌激素信号通路 Estrogen signaling pathway	1	0.047	γ氨基丁酸Gamma-aminobutyric acid

Table 3 KEGG pathway annotation

Other figure/table from this article

Fig. 1 The main nutritive components. Figures a-f show the nutritive components of Auricularia cornea cultivated with different substrates respectively. Different lowercase letters indicate the significant difference (P<0.05).
Fig. 2 PCA score plot. A: Miscellaneous sawdust, control; B: Eucalyptus sawdust; C: Eucalyptus bark. The same below.
Fig. 3 OPLS-DA score plot. Figures a, b and c show the OPLS-DA score plot of A&B, A&C and B&C respectively.
Table 1 Main evaluation indexes of OPLS-DA model
Fig. 4 OPLS-DA permutation test plots. Figures aʹ, bʹ and cʹ correspond to comparison groups A&B, A&C and B&C respectively. R² represents the cumulative variance value of the model; Q² represents the cumulative cross validation of the model; R²Y (cum) represents cumulative interpretation rate.
Table 2 Different metabolites among treatment groups
Fig. 5 Hierarchical clustering heat map. Figures a, b and c correspond to comparison groups A&B, A&C and B&C respectively. Each row represents a metabolite; Each column represents metabolites from different samples; Red indicates high metabolite content; Green indicates low metabolite content.
Fig. 6 KEGG enrichment pathway diagram. Figures a, b and c show the KEGG enrichment pathway of A&B, A&C and B&C respectively. Each bubble represents a pathway; Abscissa represents the impact value in the pathway; Ordinate represents the enrichment significance of metabolites in the pathway -log₁₀ (P-value); Bubble size represents the impact value; The larger the bubble, the greater the importance of the pathway. Bubble color from light to deep indicates that P-value decreases in turn.

模态框（Modal）标题

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